Abstract
Bone marrow-derived human mesenchymal stromal cells (hMSCs) constitute a promising therapeutic approach. However, the extremely low frequency of hMSCs in bone marrow makes the translation of these regulatory cells to clinical therapies difficult for large patient populations. Here, we describe a good manufacturing practices-compliant procedure for the expansion of hMSCs using the Quantum Cell Expansion System. This closed and automated system allows the large-scale expansion of hMSCs while maintaining their multipotency, immunophenotype, morphology, and karyotype.
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Acknowledgments
This work was supported by a Board of Visitors grant from Children’s National awarded to Catherine M. Bollard, M.D., David Jacobsohn, M.D., and Patrick J. Hanley, Ph.D. The authors would like to thank our collaborators at Baylor College of Medicine, Dr. Adrian Gee, Dr. Zhuyong Mei, and Dr. Helen Heslop. We would also like to thank the Program for Cell Enhancement and Technologies for Immunotherapy (CETI) director, Dr. Catherine Bollard, for her support and guidance.
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© 2014 Springer Science+Business Media New York
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Martin-Manso, G., Hanley, P.J. (2014). Using the Quantum Cell Expansion System for the Automated Expansion of Clinical-Grade Bone Marrow-Derived Human Mesenchymal Stromal Cells. In: Turksen, K. (eds) Stem Cells and Good Manufacturing Practices. Methods in Molecular Biology, vol 1283. Humana Press, New York, NY. https://doi.org/10.1007/7651_2014_164
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DOI: https://doi.org/10.1007/7651_2014_164
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2434-9
Online ISBN: 978-1-4939-2435-6
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