Skip to main content
SpringerLink
Log in
Book cover

Stem Cell Renewal and Cell-Cell Communication pp 113–125Cite as

Generation, Expansion, and Differentiation of Cardiovascular Progenitor Cells from Human Pluripotent Stem Cells

  • Protocol
  • First Online:

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1212))

Abstract

Cardiovascular progenitor cells (CVPCs) derived from human embryonic stem cells and human induced pluripotent stem cells represent an invaluable potential source for the study of early embryonic cardiovascular development and stem cell-based therapies for congenital and acquired heart diseases. To fully realize their values, it is essential to establish an efficient and stable differentiation system for the induction of these pluripotent stem cells (PSCs) into the CVPCs and robustly expand them in culture, and then further differentiate these CVPCs into multiple cardiovascular cell types. Here we describe the protocols for efficient derivation, expansion, and differentiation of CVPCs from hPSCs in a chemically defined medium under feeder- and serum-free culture conditions.

This is a preview of subscription content, log in via an institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Thomson JA, Itskovitz-Eldor J, Shapiro SS et al (1998) Embryonic stem cell lines derived from human blastocysts. Science 282:1145–1147

    Article  CAS  PubMed  Google Scholar 

  2. Takahashi K, Tanabe K, Ohnuki M et al (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131:861–872

    Article  CAS  PubMed  Google Scholar 

  3. Park IH, Lerou PH, Zhao R et al (2008) Generation of human-induced pluripotent stem cells. Nat Protoc 3:1180–1186

    Article  CAS  PubMed  Google Scholar 

  4. Burridge PW, Keller G, Gold JD et al (2012) Production of de novo cardiomyocytes: human pluripotent stem cell differentiation and direct reprogramming. Cell Stem Cell 10:16–28

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  5. Murry CE, Keller G (2008) Differentiation of embryonic stem cells to clinically relevant populations: lessons from embryonic development. Cell 132:661–680

    Article  CAS  PubMed  Google Scholar 

  6. Blin G, Neri T, Stefanovic S et al (2010) Human embryonic and induced pluripotent stem cells in basic and clinical research in cardiology. Curr Stem Cell Res Ther 5:215–226

    Article  CAS  PubMed  Google Scholar 

  7. Noseda M, Peterkin T, Simoes FC et al (2011) Cardiopoietic factors: extracellular signals for cardiac lineage commitment. Circ Res 108:129–152

    Article  CAS  PubMed  Google Scholar 

  8. Mummery CL, Zhang J, Ng ES et al (2012) Differentiation of human embryonic stem cells and induced pluripotent stem cells to cardiomyocytes: a methods overview. Circ Res 111:344–358

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  9. Bu L, Jiang X, Martin-Puig S et al (2009) Human ISL1 heart progenitors generate diverse multipotent cardiovascular cell lineages. Nature 460:113–117

    Article  CAS  PubMed  Google Scholar 

  10. Yang L, Soonpaa MH, Adler ED et al (2008) Human cardiovascular progenitor cells develop from a KDR+ embryonic-stem-cell-derived population. Nature 453:524–528

    Article  CAS  PubMed  Google Scholar 

  11. Blin G, Nury D, Stefanovic S et al (2010) A purified population of multipotent cardiovascular progenitors derived from primate pluripotent stem cells engrafts in postmyocardial infarcted nonhuman primates. J Clin Invest 120:1125–1139

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  12. Cao N, Liang H, Huang J et al (2013) Highly efficient induction and long-term maintenance of multipotent cardiovascular progenitors from human pluripotent stem cells under defined conditions. Cell Res 23:1119–1132

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  13. Elliott DA, Braam SR, Koutsis K et al (2011) NKX2-5(eGFP/w) hESCs for isolation of human cardiac progenitors and cardiomyocytes. Nat Methods 8:1037–1040

    Article  CAS  PubMed  Google Scholar 

  14. Kattman SJ, Witty AD, Gagliardi M et al (2011) Stage-specific optimization of activin/nodal and BMP signaling promotes cardiac differentiation of mouse and human pluripotent stem cell lines. Cell Stem Cell 8:228–240

    Article  CAS  PubMed  Google Scholar 

  15. Li C, Zhou J, Shi G et al (2009) Pluripotency can be rapidly and efficiently induced in human amniotic fluid-derived cells. Hum Mol Genet 18:4340–4349

    Article  CAS  PubMed  Google Scholar 

  16. Leschik J, Stefanovic S, Brinon B et al (2008) Cardiac commitment of primate embryonic stem cells. Nat Protoc 3:1381–1387

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The work was supported by grants of the Strategic Priority Research Program of CAS (XDA01020204), National Natural Science Foundation of China (31030050), National Basic Research Program of China (2011CB965300), National Science and Technology Project of China (2012ZX09501-001-001), and CAS (GJHZ1225). We are grateful to WiCell Research Institute for providing us H1 and H9 hESC lines and to Dr. Ying Jin (Institute of Health Sciences, China) for kindly providing the hiPSC line hAFDC-iPS-36. We also thank the members from our laboratory for valuable discussions.

Author information

Authors and Affiliations

  1. Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (CAS) & Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China

    Nan Cao, He Liang & Huang-Tian Yang

Authors
  1. Nan Cao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. He Liang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Huang-Tian Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Huang-Tian Yang .

Editor information

Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, Ontario, Canada

    Kursad Turksen

Rights and permissions

Reprints and permissions

Copyright information

© 2014 Springer Science+Business Media New York

About this protocol

Cite this protocol

Cao, N., Liang, H., Yang, HT. (2014). Generation, Expansion, and Differentiation of Cardiovascular Progenitor Cells from Human Pluripotent Stem Cells. In: Turksen, K. (eds) Stem Cell Renewal and Cell-Cell Communication. Methods in Molecular Biology, vol 1212. Humana Press, New York, NY. https://doi.org/10.1007/7651_2014_119

Download citation

  • DOI: https://doi.org/10.1007/7651_2014_119

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2589-6

  • Online ISBN: 978-1-4939-2590-2

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation