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Differentiation of Epidermal Keratinocytes from Human Embryonic Stem Cells

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Epidermal Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1195))

Abstract

For many years, cell therapies have been hampered by limited availability and inter-batch variability of primary cells. Human embryonic stem cell (hESC) can give rise to specialized cells like keratinocytes and recently emerged as a virtually unlimited source of potential therapeutic cells. However, xenogeneic components in differentiation cocktails have been limiting the clinical potential of hESC-derived keratinocytes (hESCs-Kert). Here, we demonstrated efficient differentiation of H9 human embryonic stem cells (H9-hESCs) into keratinocytes (H9-KertACC) in an autogenic co-culture system. We used activin as the main factor to induce keratinocyte differentiation. H9-KertACC expressed keratinocyte markers at mRNA and protein levels. Establishment of such animal-free microenvironment for keratinocyte differentiation will accelerate potential clinical application of hESCs.

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Author information

Authors and Affiliations

  1. Institute for Integrated Cell-Material Sciences, Kyoto University, Kyoto, Japan

    Kai Lu

  2. Oral Sciences Disciplines, Faculty of Dentistry, National University of Singapore, Singapore, Singapore

    Fahad K. Kidwai & Tong Cao

Authors
  1. Fahad K. Kidwai
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  2. Tong Cao
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  3. Kai Lu
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Corresponding author

Correspondence to Kai Lu .

Editor information

Editors and Affiliations

  1. Sprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, Ontario, Canada

    Kursad Turksen

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© 2013 Springer Science+Business Media New York

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Kidwai, F.K., Cao, T., Lu, K. (2013). Differentiation of Epidermal Keratinocytes from Human Embryonic Stem Cells. In: Turksen, K. (eds) Epidermal Cells. Methods in Molecular Biology, vol 1195. Springer, New York, NY. https://doi.org/10.1007/7651_2013_46

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  • DOI: https://doi.org/10.1007/7651_2013_46

  • Published:

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4939-1223-0

  • Online ISBN: 978-1-4939-1224-7

  • eBook Packages: Springer Protocols

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