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Origin, Development and Structure of Somatic Embryosin Selected Bulbous Ornamentals: BAP as Inducer

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Somatic Embryogenesis

Part of the book series: Plant Cell Monographs ((CELLMONO,volume 2))

Abstract

Somatic embryogenesis in three important ornamentals is discussed in this chapter. Direct somatic embryo development on the explant tissues (bulb-scale) was noticed in Hippeastrum hybridum and Eucharis grandiflora, both of which are members of Amaryllidaceae. At the time of initiation the embryos were small, water droplet-like, opaque structures, and such development was entirely restricted to the outer rows of scales only. In Crinum asiaticum, callus-mediated (indirect) embryo formation was observed on the flower-bud callus, whereas the bulb-scale callus was largely non-embryogenic. Plant growth regulator, such as 6-benzylaminopurine (BAP), frequently induced somatic embryos in Hippeastrum and Eucharis, and the addition of naphthaleneacetic acid (NAA) further increased the frequency of somatic embryo production. Unlike in many other plant systems, 2,4-D had little or no role in inducing somatic embryogenesis in Hippeastrum and Eucharis. The somatic embryos eventually gave rise to individual plantlets, though occasionally exhibiting dormancy. Histological and scanning electron microscopic observations during the stages of embryo development are presented. The embryo-derived plants had normal chromosome numbers. Besides the importance of true-to-type propagation, somatic embryogenesis offers a system of studying the various facets of non-zygotic embryo development.

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Authors and Affiliations

  1. Department of Botany, Hamdard University, 110062, New Delhi, India

    A. Mujib

  2. CSIRO Publishing, 3066, Melbourne, VIC, Australia

    S. Banerjee

  3. Department of Botany, Kalyani University, 741 235, Kalyani, West Bengal, India

    P. D. Ghosh

Authors
  1. A. Mujib
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  2. S. Banerjee
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  3. P. D. Ghosh
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Corresponding author

Correspondence to A. Mujib .

Editor information

A. Mujib Jozef Šamaj

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Mujib, A., Banerjee, S., Ghosh, P.D. Origin, Development and Structure of Somatic Embryosin Selected Bulbous Ornamentals: BAP as Inducer. In: Mujib, A., Šamaj, J. (eds) Somatic Embryogenesis. Plant Cell Monographs, vol 2. Springer, Berlin, Heidelberg. https://doi.org/10.1007/7089_028

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