Skip to main content

Chemical- and Irradiation-Induced Mutants and TILLING

  • Chapter
Rice Functional Genomics

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Abe T, Hayashi Y, Saito H, Takehisa H, Miyazawa Y, Yamamoto YY, Ryuto H, Fukunishi N, Sato T, Yoshida S, Kameya T (2005) Chlorophyll-deficient mutants of rice induced by C-ion irradiation. RIKEN Accel Prog Rep 38:132

    Google Scholar 

  • Abe T, Yasuda M, Takehisa H, Hayashi Y, Saito H, Ichida H, Shirao T, Onuma R, Ryuto H, Fukunishi N, Miyazawa Y, Tokairin H, Nakashida H, Kudo T, Sato T (2006) Isolation of morphological mutants of rice induced by heavy-ion irradiation, RIKEN Accel Prog Rep 39:137

    Google Scholar 

  • Ashburner M (1990) Drosophila, A Laboratory Handbook. Cold Spring Harbor, NY, Cold Spring Harbor Press

    Google Scholar 

  • Ashikari M, Wu J, Yano M, Sasaki T, Yoshimura A (1999) Rice gibberellin-insensitive dwarf mutant geneDwarf 1 encodes the alpha-subunit of GTP-binding protein. Proc Natl Acad Sci USA 96:10284–10289

    Article  PubMed  CAS  Google Scholar 

  • Auerbach C, Robson JM (1946) Chemical production of mutations. Nature 157:302

    CAS  Google Scholar 

  • Bentley A, MacLennan B, Calvo J, Dearolf CR (2000) Targeted recovery of mutations in Drosophila. Genetics 156:1169–1173

    PubMed  CAS  Google Scholar 

  • Bhattramakki D, Dolan Hanafey M, Wineland R, Vaske D, Register J, Tingey S, Rafalski A (2002) Insertion-deletion polymorphisms in 3’ regions of maize genes occur frequently and can be used as highly informative markers. Plant Mol Biol 48:539–547

    Article  PubMed  CAS  Google Scholar 

  • Blakely EA, Kronenberg A (1998) Heavy-ion radiobiology: new approaches to delineate mechanisms underlying enhanced biological effectiveness. Radiat Res 150:S126–145

    Article  PubMed  CAS  Google Scholar 

  • Borevitz JO, Liang D, Plouffe D, Chang HS, Zhu T, Weigel D, Berry CC, Winzeler E, Chory J (2003) Large-scale identification of single-feature polymorphisms in complex genomes. Genome Res 13:513–523

    Article  PubMed  CAS  Google Scholar 

  • Bruggemann E, Handwerger K, Essex C, Storz G (1996) Analysis of fast neutron-generated mutants at the Arabidopsis thaliana HY4 locus. Plant J 10:755–760

    Article  PubMed  CAS  Google Scholar 

  • Cecchini E, Mulligan BJ, Covey SN, Milner JJ (1998) Characterization of gamma irradiation-induced deletion mutations at a selectable locus in Arabidopsis. Mutat Res 401:199–206

    PubMed  CAS  Google Scholar 

  • Chang HS, Wu C, Zeng L, Dunn M, Wang GL, Leung H, Goff S, Wang X, Zhu T, Leach JE (2003) Detection of deleted genes in rice mutants using the Rice GeneChip genome array. In: Abstracts of Plant and Animal Genome XI. 11-15 January 2003, San Diego, California, p 100

    Google Scholar 

  • Cheema AA, Atta BM (2003) Radioactivity studies in Basmati rice. Pak J Bot 35:197–207

    CAS  Google Scholar 

  • Comai L, Henikoff S (2006) TILLING: practical single-nucleotide mutation discovery. Plant J 45:684–694

    Article  PubMed  CAS  Google Scholar 

  • Comai L, Young K, Till B, Reynolds S, Greene E, Codomo C, Enns L, Johnson J, Burtner C, Odden A, Henikoff S (2004) Efficient discovery of DNA polymorphisms in natural populations by Ecotilling. Plant J 37:778–786

    Article  PubMed  CAS  Google Scholar 

  • Cooper J, Till B, Codomo C, Burtner C, Young K, Bowers E, Holm A, Laport R, Greene E, Zerr T, Kwong S, Comai L, Henikoff S (2005) TILLING and Ecotilling in Rice In: Plant Biology 2005 Symposium VI: New Directions of Rice Research in Post-Genome Sequencing Era, Seattle, Washington, USA

    Google Scholar 

  • Delatte T, Umhang M, Trevisan M, Eicke S, Thorneycroft D, Smith SM, Zeeman SC (2006) Evidence for distinct mechanisms of starch granule breakdown in plants. J Biol Chem 281:12050–12059

    Article  PubMed  CAS  Google Scholar 

  • Ehrenberg L, Hussain S (1981) Genetic toxicity of some important epoxides. Mutat Res 86:1–113

    PubMed  CAS  Google Scholar 

  • Giaever G, Chu AM, Ni L, Connelly C, Riles L, Veronneau S, Dow S, Lucau-Danila A, Anderson K, Andre B, Arkin AP, Astromoff A, El-Bakkoury M, Bangham R, Benito R, Brachat S, Campanaro S, Curtiss M, Davis K, Deutschbauer A, Entian KD, Flaherty P, Foury F, Garfinkel DJ, Gerstein M, Gotte D, Guldener U, Hegemann JH, Hempel S, Herman Z, Jaramillo DF, Kelly DE, Kelly SL, Kotter P, LaBonte D, Lamb DC, Lan N, Liang H, Liao H, Liu L, Luo C, Lussier M, Mao R, Menard P, Ooi SL, Revuelta JL, Roberts CJ, Rose M, Ross-Macdonald P, Scherens B, Schimmack G, Shafer B, Shoemaker DD, Sookhai-Mahadeo S, Storms RK, Strathern JN, Valle G, Voet M, Volckaert G, Wang CY, Ward TR, Wilhelmy J, Winzeler EA, Yang Y, Yen G, Youngman E, Yu K, Bussey H, Boeke JD, Snyder M, Philippsen P, Davis RW, Johnston M (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418:387–391

    Article  PubMed  CAS  Google Scholar 

  • Gifford ML, Robertson FC, Soares DC, Ingram GC (2005) ARABIDOPSIS CRINKLY4function, internalization, and turnover are dependent on the extracellular Crinkly repeat domain. Plant Cell 17:1154 –1166

    Article  PubMed  CAS  Google Scholar 

  • Gilchrist E, Haughn G (2005) TILLING without a plough: a new method with applications for reverse genetics. Curr Opin Plant Biol 8:211–215

    Article  PubMed  CAS  Google Scholar 

  • Gilchrist EJ, Haughn GW, Ying CE, Otto SP, Zhuang J, Cheung D, Hamberger B, Aboutorabi F, Kalynyak T, Johnson L, Bohlmann J, Ellis BC, Douglas CJ, Cronk QC (2006) Use of Ecotilling as an efficient SNP discovery tool to survey genetic variation in wild populations of Populus trichocarpa Molecular Ecology 15:1367–1378

    Article  PubMed  CAS  Google Scholar 

  • Goff S, Ricke D, Lan T, Presting G, Wang R, Dunn M, Glazebrook J, Sessions A, Oeller P, Varma H, Hadley D, Hutchison D, Martin C, Katagiri F, Lange B, Moughamer T, Xia Y, Budworth P, Zhong J, Miguel T, Paszkowski U, Zhang S, Colbert M, Sun W, Chen L, Cooper B, Park S, Wood T, Mao L, Quail P, Wing R, Dean R, Yu Y, Zharkikh A, Shen R, Sahasrabudhe S, Thomas A, Cannings R, Gutin A, Pruss D, Reid J, Tavtigian S, Mitchell J, Eldredge G, Scholl T, Miller R, Bhatnagar S, Adey N, Rubano T, Tusneem N, Robinson R, Feldhaus J, Macalma T, Oliphant A, Briggs S (2002) A draft sequence of the rice genome (Oryza sativaL. ssp.japonica). Science 296:92–100

    Article  PubMed  CAS  Google Scholar 

  • Goll MG, Bestor TH (2002) Histone modification and replacement in chromatin activation. Genes Dev 16:1739–1742

    Article  PubMed  CAS  Google Scholar 

  • Gong J-M, Waner D, Horie T, Li S, Horie R, Abid K, Schroeder J (2004) Microarray-based rapid cloning of an ion accumulation deletion mutant in Arabidopsis thaliana. Proc Natl Acad Sci USA 101:15404–15409

    Article  PubMed  CAS  Google Scholar 

  • Goodhead DT (1995) Molecular and cell models of biological effects of heavy ion radiation. Radiat Environ Biophys 34:67–72

    Article  PubMed  CAS  Google Scholar 

  • Graf U, Wurgler FE, Katz AJ, Frei H, Juon H, Hall CB, Kale PG (1984) Somatic mutation and recombination test in Drosophila melanogaster. Environ Mutagen 6:153–188

    Article  PubMed  CAS  Google Scholar 

  • Guenet J (2004) Chemical mutagenesis of the mouse genome: an overview. Genetica 122:9–24

    Article  PubMed  CAS  Google Scholar 

  • Hagen U (1994) Mechanisms of induction and repair of DNA double-strand breaks by ionizing radiation: some contradictions. Radiat Environ Biophys 33:45–61

    Article  PubMed  CAS  Google Scholar 

  • Hase Y, Tanaka A, Baba T, Watanabe H (2000) FRL1 is required for petal and sepal development in Arabidopsis. Plant J 24:21–32

    Article  PubMed  CAS  Google Scholar 

  • Henikoff S, Comai L (2003) Single-nucleotide mutations for plant functional genomics. Annu Rev Plant Biol 54:375–401

    Article  PubMed  CAS  Google Scholar 

  • Henikoff S, Till B, Comai L (2004) TILLING. Traditional mutagenesis meets functional genomics. Plant Physiol 135:630–636

    CAS  Google Scholar 

  • Hieter P, Boguski M (1997) Functional genomics: it’s all how you read it. Science 278:601–602

    Article  PubMed  CAS  Google Scholar 

  • Jansen G, Hazendonk E, Thijssen K, Plasterk R (1997) Reverse genetics by chemical mutagenesis in Caenorhabditis elegans. Nat Genet 17:119–121

    Article  PubMed  CAS  Google Scholar 

  • Kieber JJ, Rothenberg M, Roman G, Feldmann KA, Ecker JR (1993) CTR1, a negative regulator of the ethylene response pathway in Arabidopsis, encodes a member of the Raf family of protein kinases. Cell 72:427–441

    Article  PubMed  CAS  Google Scholar 

  • Kim J, DellaPenna D (2006) Defining the primary route for lutein synthesis in plants: The role of Arabidopsis carotenoid beta-ring hydroxylase CYP97A3. Proc Natl Acad Sci USA 103:3474 –3479

    Article  PubMed  CAS  Google Scholar 

  • Kitamura S, Shikazono N, Tanaka A (2004) TRANSPARENT TESTA 19 is involved in the accumulation of both anthocyanins and proanthocyanidins in Arabidopsis. Plant J 37:104–114

    Article  PubMed  CAS  Google Scholar 

  • Koornneef M, Dellaert LW, van der Veen JH (1982) EMS- and radiation-induced mutation frequencies at individual loci in Arabidopsis thaliana (L.) Heynh. Mutat Res 93:109–123

    PubMed  CAS  Google Scholar 

  • Kraft G, Kramer M, Scholz M (1992) LET, track structure and models. A review. Radiat Environ Biophys 31:161–180

    Article  CAS  Google Scholar 

  • Kurata N, Yamazaki Y (2006) Oryzabase. An integrated biological and genome information database for rice. Plant Physiol 140:12–17

    Article  PubMed  CAS  Google Scholar 

  • Leung H, Wu C, Baraoidan M, Bordeos A, Ramos M, Madamba S, Cabauatan P, Vera Cruz C, Portugal A, Reves G, Bruskiewich R, McLaren G, Gregorio G, Bennett J, Brar D, Khush G, Schnable P, Wang G, Leach J (2001) Deletion mutants for functional genomics: Progress in phenotyping, sequence assignment, and database development. In: Khush G, Brar D, Hardy B (eds) Rice Genetics IV. Science Publishers, New Delhi, pp 239–251

    Google Scholar 

  • Li B, Conway N, Navarro S, Comai L (2005) A conserved and species-specific functional interaction between the Werner syndrome-like exonuclease at WEX and the Ku heterodimer in Arabidopsis. Nucl Acids Res 33:6861– 6867

    Article  PubMed  CAS  Google Scholar 

  • Li Q, Liu Z, Monroe H, Culiat CT (2002) Integrated platform for detection of DNA sequence variants using capillary array electrophoresis. Electrophoresis 23:1499–1511

    Article  PubMed  CAS  Google Scholar 

  • Li X, Zhang Y (2002) Reverse genetics by fast neutron mutagenesis in higher plants. Funct Integr Genomics 2:254–258

    Article  PubMed  CAS  Google Scholar 

  • Li X, Song Y, Century K, Straight S, Ronald P, Dong X, Lassner M, Zhang Y (2001) A fast neutron deletion mutagenesis-based reverse genetics system for plants. Plant J 27:235–242

    Article  PubMed  CAS  Google Scholar 

  • Liu B, Zhang S, Zhu X, Yang Q, Wu S, Mei M, Mauleon R, Leach J, Mew T, Leung H (2004) Candidate defense genes as predictors of quantitative blast resistance in rice. Mol Plant-Microbe Interact 17:1146–1152

    PubMed  CAS  Google Scholar 

  • Liu LX, Spoerke JM, Mulligan EL, Chen J, Reardon B, Westlund B, Sun L, Abel K, Armstrong B, Hardiman G, King J, McCague L, Basson M, Clover R, Johnson CD (1999) High-throughput isolation ofCaenorhabditis elegans deletion mutants. Genome Res 9:859–867

    Article  PubMed  CAS  Google Scholar 

  • McCallum CM, Comai L, Greene EA, Henikoff S (2000a) Targeting induced local lesions in genomes (TILLING) for plant functional genomics. Plant Physiol 123:439–442

    Article  CAS  Google Scholar 

  • McCallum CM, Comai L, Greene EA, Henikoff S (2000b) Targeted screening for induced mutations. Nat Biotechnol 18:455–457

    Article  CAS  Google Scholar 

  • Monna L, Ohta R, Masuda H, Koike A, Minobe Y (2006) Genome-wide searching of single-nucleotide polymorphisms among eight distantly and closely related rice cultivars (Oryza sativa L.) and a wild accession (Oryza rufipogon Griff.). DNA Res 13:43–51

    Article  PubMed  CAS  Google Scholar 

  • Morris ER, Chevalier D, Walker JC (2006) DAWDLE, a Forkhead-associated domain gene, regulates multiple aspects of plant development. Plant Physiol 141:932–941

    Article  PubMed  CAS  Google Scholar 

  • Nadeau JH, Frankel WN (2000) The roads from phenotypic variation to gene discovery: mutagenesis versus QTLs. Nat Genet 25:381–384

    Article  PubMed  CAS  Google Scholar 

  • Nambara E, Keith K, McCourt P, Naito S (1994) Isolation of an internal deletion mutant of the Arabidopsis thaliana ABI3 gene. Plant Cell Physiol 35:509–513

    PubMed  CAS  Google Scholar 

  • Nikjoo H, Uehara S, Wilson WE, Hoshi M, Goodhead DT (1998) Track structure in radiation biology: theory and applications. Int J Radiat Biol 73:355–364

    Article  PubMed  CAS  Google Scholar 

  • Olsen OA, Green MM (1982) The mutagenic effects of diepoxybutane in wild-type and mutagen-sensitive mutants of Drosophila melanogaster. Mutat Res 92:107–115

    PubMed  CAS  Google Scholar 

  • Olsen O, Wang X, von Wettstein D (1993) Sodium azide mutagenesis: Preferential generation of A·T G·C transitions in the barley Ant18 Gene. Proc Natl Acad Sci USA, 90:8043–8047.

    Article  PubMed  CAS  Google Scholar 

  • Oppenheimer LW, Farine D, Ritchie JW, Lewinsky RM, Telford J, Fairbanks LA (1991) What is a low-lying placenta? Am J Obstet Gynecol 165:1036–1038

    PubMed  CAS  Google Scholar 

  • Perry J, Wang T, Welham T, Gardner S, Pike J, Yoshida S, Parniske M (2003) A TILLING reverse genetics tool and a web-accessible collection of mutants of the legume Lotus japonicus. Plant Physiol 131:866–871

    Article  PubMed  CAS  Google Scholar 

  • Peters JL, Cnudde F, Gerats T (2003) Forward genetics and map-based cloning approaches. Trends Plant Sci 8:484–491

    Article  PubMed  CAS  Google Scholar 

  • Raghavan C, Naredo E, Wang H, Atienza G, Liu B, Qiu F, McNally K, Leung H (2007) Rapid method for detecting SNPs on agarose gels and its application in candidate gene mapping. Molecular Breeding 19:87–101

    Article  CAS  Google Scholar 

  • Ramalingam J, Vera Cruz C, Kukreja K, Chittoor J, Wu J, Lee S, Baraoidan M, George M, Cohen M, Hulbert S, Leach J, Leung H (2003) Candidate resistance genes from rice, barley, and maize and their association with qualitative and quantitative resistance in rice. Mol Plant-Microbe Interact 16:14–24

    PubMed  CAS  Google Scholar 

  • Reardon JT, Liljestrand-Golden CA, Dusenbery RL, Smith PD (1987) Molecular analysis of diepoxybutane-induced mutations at the rosy locus of Drosophila melanogaster. Genetics 115:323–331

    PubMed  CAS  Google Scholar 

  • Resnick JS, Wen C-K, Shockey JA, Chang C (2006) REVERSION-TO-ETHYLENE SENSITIVITY1, a conserved gene that regulates ethylene receptor function in Arabidopsis. Proc Natl Acad Sci USA 103:7917–7922

    Article  PubMed  CAS  Google Scholar 

  • Sakamoto A, Lan VT, Hase Y, Shikazono N, Matsunaga T, Tanaka A (2003) Disruption of the AtREV3 gene causes hypersensitivity to ultraviolet B light and gamma-rays in Arabidopsis: implication of the presence of a translesion synthesis mechanism in plants. Plant Cell 15:2042–2057

    Article  PubMed  CAS  Google Scholar 

  • Salmeron JM, Oldroyd GE, Rommens CM, Scofield SR, Kim HS, Lavelle DT, Dahlbeck D, Staskawicz BJ (1996) TomatoPrf is a member of the leucine-rich repeat class of plant disease resistance genes and lies embedded within the Pto kinase gene cluster. Cell 86:123–133

    Article  PubMed  CAS  Google Scholar 

  • Sarma NP, Patnaik A, Jachuck PJ (1979) Azide mutagenesis in rice: Effect of concentration and soaking time on induced chlorophyll mutation frequency Environ Exp Bot 19:117–121

    Article  CAS  Google Scholar 

  • Sega G (1984) A review of the genetic effects of ethyl methanesulfonate. Mutat Res 134:113–142

    PubMed  CAS  Google Scholar 

  • Shen YJ, Jiang H, Jin JP, Zhang ZB, Xi B, He YY, Wang G, Wang C, Qian L, Li X, Yu QB, Liu HJ, Chen DH, Gao JH, Huang H, Shi TL, Yang ZN (2004) Development of genome-wide DNA polymorphism database for map-based cloning of rice genes. Plant Physiol 135:1198–1205

    Article  PubMed  CAS  Google Scholar 

  • Shikazono N, Yokota Y, Tanaka A, Watanabe H, Tano S (1998) Molecular analysis of carbon ion-induced mutations in Arabidopsis thaliana. Genes Genet Syst 73:173–179

    Article  PubMed  CAS  Google Scholar 

  • Shikazono N, Tanaka A, Watanabe H, Tano S (2001) Rearrangements of the DNA in carbon ion-induced mutants of Arabidopsis thaliana. Genetics 157:379–387

    PubMed  CAS  Google Scholar 

  • Shikazono N, Yokota Y, Kitamura S, Suzuki C, Watanabe H, Tano S, Tanaka A (2003) Mutation rate and novel tt mutants of Arabidopsis thaliana induced by carbon ions. Genetics 163:1449–1455

    PubMed  CAS  Google Scholar 

  • Shikazono N, Suzuki C, Kitamura S, Watanabe H, Tano S, Tanaka A (2005) Analysis of mutations induced by carbon ions inArabidopsis thaliana. J Exp Bot 56:587–596

    Article  PubMed  CAS  Google Scholar 

  • Shirley BW, Hanley S, Goodman HM (1992) Effects of ionizing radiation on a plant genome: analysis of two Arabidopsis transparent testa mutations. Plant Cell 4:333–347

    Article  PubMed  CAS  Google Scholar 

  • Shukla PT, Auerbach C (1980) Genetic tests for the detection of chemically induced small deletions in Drosophila chromosomes. Mutat Res 72:231–243

    PubMed  CAS  Google Scholar 

  • Slade A, Fuerstenberg S, Loeffler D, Steine M, Facciotti D (2005) A reverse genetic, nontransgenic approach to wheat crop improvement by TILLING. Nat Biotechnol 23:75–81

    Article  PubMed  CAS  Google Scholar 

  • Smith HH (1972) Comparative genetic effects of different physical mutagens in higher plants. In: Induced Mutations and Plant Improvement, International Atomic Energy Agency, Vienna, pp 75–93

    Google Scholar 

  • Sun T, Goodman HM, Ausubel FM (1992) Cloning the Arabidopsis GA1 Locus by genomic subtraction. Plant Cell 4:119–128

    Article  PubMed  CAS  Google Scholar 

  • Suzuki T, Eiguchi M, Satoh H, Kumamaru T, Kurata N (2005) A modified TILLING system for rice mutant screening. Rice Genet Newsl 22:89–91

    Google Scholar 

  • Tanaka A, Tano S, Chantes T, Yokota Y, Shikazono N, Watanabe H (1997) A new Arabidopsis mutant induced by ion beams affects flavonoid synthesis with spotted pigmentation in testa. Genes Genet Syst 72:141–148

    Article  PubMed  CAS  Google Scholar 

  • Till B, Colbert T, Tompa R, Enns L, Codomo C, Johnson J, Reynolds S, Henikoff J, Greene E, Steine M, Comai L, Henikoff S (2003a) High-throughput TILLING for functional genomics. Methods Mol Biol 236:205–220

    CAS  Google Scholar 

  • Till B, Reynolds S, Greene E, Codomo C, Enns L, Johnson J, Burtner C, Odden A, Young K, Taylor N, Henikoff J, Comai L, Henikoff S (2003b) Large-scale discovery of induced point mutations with high-throughput TILLING. Genome Res 13:524–530

    Article  CAS  Google Scholar 

  • Till B, Reynolds S, Weil C, Springer N, Burtner C, Young K, Bowers E, Codomo C, Enns L, Odden A, Greene E, Comai L, Henikoff S (2004) Discovery of induced point mutations in maize genes by TILLING. BMC Plant Biol 4:12

    Article  PubMed  Google Scholar 

  • Vogel E, Natarajan A (1995) DNA damage and repair in somatic and germ cells in vivo. Mutat Res 330:183–208

    PubMed  CAS  Google Scholar 

  • Wang GL, Wu C, Zeng L, He C, Baraoidan M, de Assis Goes da Silva F, Williams CE, Ronald PC, Leung H (2004) Isolation and characterization of rice mutants compromised in Xa21-mediated resistance toX. oryzae pv. oryzae. Theor Appl Genet 108:379–384

    Article  PubMed  CAS  Google Scholar 

  • Wang H (2005) Application of EcoTILLING to relate molecular variation in a rice ethylene response factor (ERF3) gene to drought stress response. M.S. Thesis, University of the Philippines, Los Banos, Philippines, p 86

    Google Scholar 

  • Watson WAF (1966) Further evidence of an essential difference between the genetical effects of mono- and bifunctional alkylating agents. Mutat Res 3:452–455

    Google Scholar 

  • Wienholds E, Schulte-Merker S, Walderich B, Plasterk RH (2002) Target-selected inactivation of the zebrafish rag1 gene. Science 297:99–102

    Article  PubMed  CAS  Google Scholar 

  • Wienholds E, van Eeden F, Kosters M, Mudde J, Plasterk R, Cuppen E (2003) Efficient target-selected mutagenesis in zebrafish. Genome Res 13:2700–2707

    Article  PubMed  CAS  Google Scholar 

  • Wilkinson JQ, Crawford NM (1991) Identification of the Arabidopsis CHL3 gene as the nitrate reductase structural gene NIA2. Plant Cell 3:461–471

    Article  PubMed  CAS  Google Scholar 

  • Winkler S, Schwabedissen A, Backasch D, Bokel C, Seidel C, Bonisch S, Furthauer M, Kuhrs A, Cobreros L, Brand M, Gonzalez-Gaitan M (2005) Target-selected mutant screen by TILLING in Drosophila. Genome Res 15:718–723

    Article  PubMed  CAS  Google Scholar 

  • Winzeler EA, Castillo-Davis CI, Oshiro G, Liang D, Richards DR, Zhou Y, Hartl DL (2003) Genetic diversity in yeast assessed with whole-genome oligonucleotide arrays. Genetics 163:79–89

    PubMed  CAS  Google Scholar 

  • Wu JL, Wu C, Lei C, Baraoidan M, Bordeos A, Madamba MR, Ramos-Pamplona M, Mauleon R, Portugal A, Ulat VJ, Bruskiewich R, Wang G, Leach J, Khush G, Leung H (2005) Chemical- and irradiation-induced mutants ofindicarice IR64 for forward and reverse genetics. Plant Mol Biol 59:85–97

    Article  PubMed  CAS  Google Scholar 

  • Yu J, Hu S, Wang J, Wong G, Li S, Liu B, Deng Y, Dai L, Zhou Y, Zhang X, Cao M, Liu J, Sun J, Tang J, Chen Y, Huang X, Lin W, Ye C, Tong W, Cong L, Geng J, Han Y, Li L, Li W, Hu G, Huang X, Li W, Li J, Liu Z, Li L, Liu J, Qi Q, Liu J, Li L, Li T, Wang X, Lu H, Wu T, Zhu M, Ni P, Han H, Dong W, Ren X, Feng X, Cui P, Li X, Wang H, Xu X, Zhai W, Xu Z, Zhang J, He S, Zhang J, Xu J, Zhang K, Zheng X, Dong J, Zeng W, Tao L, Ye J, Tan J, Ren X, Chen X, He J, Liu D, Tian W, Tian C, Xia H, Bao Q, Li G, Gao H, Cao T, Wang J, Zhao W, Li P, Chen W, Wang X, Zhang Y, Hu J, Wang J, Liu S, Yang J, Zhang G, Xiong Y, Li Z, Mao L, Zhou C, Zhu Z, Chen R, Hao B, Zheng W, Chen S, Guo W, Li G, Liu S, Tao M, Wang J, Zhu L, Yuan L, Yang H (2002) A draft sequence of the rice genome (Oryza sativa L. ssp. indica). Science 296:79–92

    PubMed  CAS  Google Scholar 

  • Zimmering S (1983) The mei-9a test for chromosome loss in Drosophila: a review of assays of 21 chemicals for chromosome breakage. Environ Mutagen 5:907–921

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

Copyright information

© 2007 Springer Science+Business Media, LLC

About this chapter

Cite this chapter

Bhat, R.S. et al. (2007). Chemical- and Irradiation-Induced Mutants and TILLING. In: Rice Functional Genomics. Springer, New York, NY. https://doi.org/10.1007/0-387-48914-2_8

Download citation

Publish with us

Policies and ethics