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Pseudomonas aeruginosa

  • SECTION 3.3 Gamma Subclass
  • Reference work entry
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The Prokaryotes

The Gram-negative bacillus Pseudomonas aeruginosa is a remarkably adaptable organism. Endowed with a large repertoire of metabolic pathways, P. aeruginosa is able to adapt, survive and persist under a broad range of environmental conditions. Equipped with an equally large repertoire of pathogenic mechanisms, P. aeruginosa is capable of infecting eukaryotic organisms ranging from social amoeba to humans. The discussion in this chapter will highlight the metabolic and pathogenic diversity of P. aeruginosa and the relationship to human infection.

Cellular Biology and Basic Physiology

Pseudomonas aeruginosa was first isolated from a variety of environmental sources by Schroeter in 1872 (Palleroni, 1984). Taxonomy based on 16S rRNA sequences positions P. aeruginosa within the class of Gammaproteobacteria and the family Pseudomonadaceae. Like all members of this family, P. aeruginosa is metabolically versatile and utilizes over 80 organic compounds as energy and carbon sources (Palleroni,...

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Literature Cited

  • Albus, A. M., E. C. Pesci, L. J. Runyen-Janecky, S. E. West, and B. H. Iglewski. 1997 Vfr controls quorum sensing in Pseudomonas aeruginosa J. Bacteriol. 179 3928–3935

    CAS  PubMed  PubMed Central  Google Scholar 

  • Allured, V. S., R. J. Collier, S. F. Carroll, and D. B. McKay. 1986 Structure of exotoxin A of Pseudomonas aeruginosa at 3.0-Angstrom resolution Proc. Natl. Acad. Sci. USA 83 1320–1324

    CAS  PubMed  Google Scholar 

  • Barbieri, J. T. 2000 Pseudomonas aeruginosa exoenzyme S, a bifunctional type-III secreted cytotoxin Int. J. Med. Microbiol. 290 381–387

    CAS  PubMed  Google Scholar 

  • Boles, B. R., M. Thoendel, and P. K. Singh. 2004 Self-generated diversity produces “insurance effects” in biofilm communities Proc. Natl. Acad. Sci. USA 101(47) 16630–16635

    CAS  PubMed  Google Scholar 

  • Bradley, D. E. 1980 A function of Pseudomonas aeruginosa PAO polar pili: Twitching motility Can. J. Microbiol. 26 146–154

    CAS  PubMed  Google Scholar 

  • Cabrol, S., A. Olliver, G. B. Pier, A. Andremont, and R. Ruimy. 2003 Transcription of quorum-sensing system genes in clinical and environmental isolates of Pseudomonas aeruginosa J. Bacteriol 185 7222–7230

    CAS  PubMed  PubMed Central  Google Scholar 

  • Cao, H., G. Krishnan, B. Goumnerov, J. Tsongalis, R. Tompkins, and L. G. Rahme. 2001 A quorum sensing-associated virulence gene of Pseudomonas aeruginosa encodes a LysR-like transcription regulator with a unique self-regulatory mechanism Proc. Natl. Acad. Sci. USA 98 14613–14618

    CAS  PubMed  Google Scholar 

  • Chugani, S. A., M. Whiteley, K. M. Lee, D. D’Argenio, C. Manoil, and E. P. Greenberg. 2001 QscR, a modulator of quorum-sensing signal synthesis and virulence in Pseudomonas aeruginosa Proc. Natl. Acad. Sci. USA 98 2752–2757

    CAS  PubMed  Google Scholar 

  • Coburn, J., A. V. Kane, L. Feig, and D. M. Gill. 1991 Pseudomonas aeruginosa exoenzyme S requires a eukaryotic protein for ADP-ribosyltransferase activity J. Biol. Chem. 266 6438–6446

    CAS  PubMed  Google Scholar 

  • Costerton, J. W., P. S. Stewart, and E. P. Greenberg. 1998 Bacterial biofilms: A common cause of persistent infections Science 284 1318–1322

    Google Scholar 

  • Crouch Brewer, S., R. G. Wunderink, C. B. Jones, and K. V. Leeper Jr. 1996 Ventilator-associated pneumonia due to Pseudomonas aeruginosa Chest 109 1019–1029

    CAS  PubMed  Google Scholar 

  • Dacheux, D., I. Attree, and B. Toussaint. 2001a Expression of ExsA in trans confers type III secretion system-dependent cytotoxicity on noncytotoxic Pseudomonas aeruginosa cystic fibrosis isolates Infect. Immun. 69 538–542

    CAS  PubMed  PubMed Central  Google Scholar 

  • Dacheux, D., J. Goure, J. Chabert, Y. Usson, and I. Attree. 2001b Pore-forming activity of type III system-secreted proteins leads to oncosis of Pseudomonas aeruginosa-infected macrophages Molec. Microbiol. 40 76–85

    CAS  Google Scholar 

  • Dasgupta, N., G. L. Lykken, M. C. Wolfgang, and T. L. Yahr. 2004 A novel anti-anti-activator mechanism regulates expression of the Pseudomonas aeruginosa type III secretion system Molec. Microbiol. 53 297–308

    CAS  Google Scholar 

  • Davey, M. E., N. C. Caiazza, and G. A. O’Toole. 2003 Rhamnolipid surfactant production affects biofilm architecture in Pseudomonas aeruginosa PAO1 J. Bacteriol 185 1027–1036

    CAS  PubMed  PubMed Central  Google Scholar 

  • Davies, D. G., M. R. Parsek, J. P. Pearson, B. H. Iglewski, J. W. Costerton, and E. P. Greenberg. 1998 The involvement of cell-to-cell signals in the development of a bacterial biofilm Science 280 295–298

    CAS  PubMed  Google Scholar 

  • Deziel, E., Y. Comeau, and R. Villemur. 2001 Initiation of biofilm formation by Pseudomonas aeruginosa 57RP correlates with emergence of hyperpiliated and highly adherent phenotypic variants deficient in swimming, swarming, and twitching motilities J. Bacteriol. 183 1195–1204

    CAS  PubMed  PubMed Central  Google Scholar 

  • Drenkard, E., and F. M. Ausubel. 2002 Pseudomonas biofilm formation and antibiotic resistance are linked to phenotypic variation Nature 416 740–743

    CAS  PubMed  Google Scholar 

  • Engel, J. E. 2003 Molecular pathogenesis of acute Pseudomonas aeruginosa infection In: A. R. Hauser and J. Rello (Eds.) Severe Infections Caused by Pseudomonas aeruginosa Kluwer Academic Publishers Boston MA 201–229

    Google Scholar 

  • Eschbach, M., K. Schreiber, K. Trunk, J. Buer, D. Jahn, and M. Schobert. 2004 Long-term anaerobic survival of the opportunistic pathogen Pseudomonas aeruginosa via pyruvate fermentation J. Bacteriol 186 4596–4604

    CAS  PubMed  PubMed Central  Google Scholar 

  • Evans, L. R., and A. Linker. 1973 Production and characterization of the slime polysaccharide of Pseudomonas aeruginosa J. Bacteriol 116 915–924

    CAS  PubMed  PubMed Central  Google Scholar 

  • Feltman, H., G. Schulert, S. Khan, M. Jain, L. Peterson, and A. R. Hauser. 2001 Prevalence of type III secretion genes in clinical and environmental isolates of Pseudomonas aeruginosa Microbiology 147 2659–2669

    CAS  PubMed  Google Scholar 

  • Finck-Barbancon, V., J. Goranson, L. Zhu, T. Sawa, J. P. Wiener-Kronish, S. M. J. Fleiszig, C. Wu, L. Mende-Mueller, and D. W. Frank. 1997 ExoU expression by Pseudomonas aeruginosa coorelates with acute cytotoxicity and epithelial injury Molec. Microbiol. 25 547–557

    CAS  Google Scholar 

  • Frank, D. W., G. Nair, and H. P. Schweizer. 1994 Construction and characterization of chromosomal insertional mutations of the Pseudomonas aeruginosa exoenzyme S trans-regulatory locus Infect. Immun. 62 554–563

    CAS  PubMed  PubMed Central  Google Scholar 

  • Friedman, L., and R. Kolter. 2004 Genes involved in matrix formation in Pseudomonas aeruginosa PA14 biofilms Molec. Microbiol. 51 675–690

    CAS  Google Scholar 

  • Gambello, M. J., S. Kaye, and B. H. Iglewski. 1993 LasR of Pseudomonas aeruginosa is a transcriptional activator of the alkaline protease gene (apr) and an enhancer of exotoxin A expression Infect. Immun. 61 1180–1184

    CAS  PubMed  PubMed Central  Google Scholar 

  • Geiser, T. K., B. I. Kazmierczak, L. K. Garrity-Ryan, M. A. Matthay, and J. N. Engel. 2001 Pseudomonas aeruginosa ExoT inhibits in vitro lung epithelial wound repair Cell Microbiol. 3 223–236

    CAS  PubMed  Google Scholar 

  • Gessard, C. 1984 Classics in infectious diseases: On the blue and green coloration that appears on bandages Rev. Infect. Dis. Suppl. 3 S775–S776

    Google Scholar 

  • Goerhing, U. M., G. Schmidt, K. J. Pederson, K. Aktories, and J. T. Barbieri. 1999 The N-terminal domain of Pseudomonas aeruginosa exoenzyme S is a GTPase-activating protein for Rho GTPases J. Biol. Chem. 274 36369–36372

    Google Scholar 

  • Goure, J., A. Pastor, E. Faudry, J. Chabert, A. Dessen, and I. Attree. 2004 The V antigen of Pseudomonas aeruginosa is required for assembly of the functional PopB/PopD translocation pore in host cell membranes Infect. Immun. 72 4741–4750

    CAS  PubMed  PubMed Central  Google Scholar 

  • Guzzo, J., J. M. Pages, F. Duong, A. Lazdunski, and M. Murgier. 1991 Pseudomonas aeruginosa alkaline protease: Evidence for secretion genes and study of secretion mechanism J. Bacteriol 173 5290–5297

    CAS  PubMed  PubMed Central  Google Scholar 

  • Hahn, H. P. 1997 The type-4 pilus is the major virulence-associated adhesin of Pseudomonas aeruginosa—a review Gene 192 99–108

    CAS  PubMed  Google Scholar 

  • Hamood, A. N., J. A. Colmer-Hamood, and N. L. Carty. 2004 Regulation of Pseudomonas aeruginosa exotoxin A synthesis In: J.-L. Ramos (Ed.) Pseudomonas, Volume 1: Genomics, Lifestyle and Molecular Architecture Kluwer Academic/Plenum Publishers New York NY 389–423

    Google Scholar 

  • Haussler, S., B. Tummler, H. Weissbrodt, M. Rohde, and I. Steinmetz. 1999 Small-colony variants of Pseudomonas aeruginosa in cystic fibrosis Clin. Infect. Dis. 29 621–625

    CAS  PubMed  Google Scholar 

  • Haussler, S., I. Ziegler, A. Lottel, F. von Gotz, M. Rohde, D. Wehmhohner, S. Saravanamuthu, B. Tummler, and I. Steinmetz. 2003 Highly adherent small-colony variants of Pseudomonas aeruginosa in cystic fibrosis lung infection J. Med. Microbiol. 52 295–301

    PubMed  Google Scholar 

  • Hentzer, M., G. M. Teitzel, G. J. Balzer, A. Heydorn, S. Molin, M. Givskov, and M. R. Parsek. 2001 Alginate overproduction affects Pseudomonas aeruginosa biofilm structure and function J. Bacteriol 183 5395–5401

    CAS  PubMed  PubMed Central  Google Scholar 

  • Hoiby, N., H. Krogh Johansen, C. Moser, Z. Song, O. Ciofu, and A. Kharazmi. 2001 Pseudomonas aeruginosa and the in vitro and in vivo biofilm mode of growth Microb. Infect. 3 23–35

    CAS  Google Scholar 

  • Holder, I. A., A. N. Neely, and D. W. Frank. 2001 Type III secretion/intoxication system important in virulence of Pseudomonas aeruginosa infections in burns Burns 27 129–130

    CAS  PubMed  Google Scholar 

  • Iglewski, B. H., and D. Kabat. 1975 NAD-dependent inhibition of protein synthesis by Pseudomonas aeruginosa toxin Proc. Natl. Acad. Sci. USA 72 2284–2288

    CAS  PubMed  Google Scholar 

  • Iglewski, B. H., J. Sadoff, M. J. Bjorn, and E. S. Maxwell. 1978 Pseudomonas aeruginosa exoenzyme S: An adenosine diphosphate ribosyltransferase distinct from toxin A Proc. Natl. Acad. Sci. USA 75 3211–3215

    CAS  PubMed  Google Scholar 

  • Jackson, K. D., M. Starkey, S. Kremer, M. R. Parsek, and D. J. Wozniak. 2004 Identification of psl, a locus encoding a potential exopolysaccharide that is essential for Pseudomonas aeruginosa PAO1 biofilm formation J. Bacteriol 186 4466–4475

    CAS  PubMed  PubMed Central  Google Scholar 

  • Klausen, M., A. Heydorn, P. Ragas, L. Lambertsen, A. Aaes-Jorgensen, S. Molin, and T. Tolker-Nielsen. 2003 Biofilm formation by Pseudomonas aeruginosa wild type, flagella and type IV pili mutants Molec. Microbiol. 48 1511–1524

    CAS  Google Scholar 

  • Kohler, T., L. K. Curty, F. Barja, C. van Delden, and J. C. Pechere. 2000 Swarming of Pseudomonas aeruginosa is dependent on cell-to-cell signaling and requires flagella and pili J. Bacteriol 182 5990–5996

    CAS  PubMed  PubMed Central  Google Scholar 

  • Kounnas, M. Z., R. E. Morris, M. R. Thompson, D. J. FitzGerald, D. K. Strickland, and C. B. Saelinger. 1992 The alpha 2-macroglobulin receptor/low density lipoprotein receptor-related protein binds and internalizes Pseudomonas exotoxin A J. Biol. Chem. 267 12420–12423

    CAS  PubMed  Google Scholar 

  • Kulich, S. M., T. L. Yahr, L. M. Mende-Mueller, J. T. Barbieri, and D. W. Frank. 1994 Cloning the structural gene for the 49-kDa form of exoenzyme S (exoS) from Pseudomonas aeruginosa strain 388 J. Biol. Chem. 269 10431–10437

    CAS  PubMed  Google Scholar 

  • Lau, G. W., H. Ran, F. Kong, D. J. Hassett, and D. Mavrodi. 2004 Pseudomonas aeruginosa pyocyanin is critical for lung infection in mice Infect. Immun. 72 4275–4278

    CAS  PubMed  PubMed Central  Google Scholar 

  • Lazdunski, A., J. Guzzo, A. Filloux, M. Bally, and M. Murgier. 1990 Secretion of extracellular proteins by Pseudomonas aeruginosa Biochimie 72 147–156

    CAS  PubMed  Google Scholar 

  • Liu, P. V. 1966 The roles of various fractions of Pseudomonas aeruginosa in its pathogenesis. 3: Identity of the lethal toxins produced in vitro and in vivo J. Infect. Dis. 116 481–489

    CAS  PubMed  Google Scholar 

  • Mah, T. F., B. Pitts, B. Pellock, G. C. Walker, P. S. Stewart, and G. A. O’Toole. 2003 A genetic basis for Pseudomonas aeruginosa biofilm antibiotic resistance Nature 426 306–310

    CAS  Google Scholar 

  • Mahenthiralingam, E., M. E. Campbell, and D. P. Speert. 1994 Nonmotility and phagocytic resistance of Pseudomonas aeruginosa isolates from chronically colonized patients with cystic fibrosis Infect. Immun. 62 596–605

    CAS  PubMed  PubMed Central  Google Scholar 

  • Matsukawa, M., and E. P. Greenberg. 2004 Putative exopolysaccharide synthesis genes influence Pseudomonas aeruginosa biofilm development J. Bacteriol 186 4449–4456

    CAS  PubMed  PubMed Central  Google Scholar 

  • May, T. B., D. Shinabarger, R. Maharaj, J. Kato, L. Chu, J. D. DeVault, S. Roychoudhury, N. A. Zielinski, A. Berry, and R. K. Rothmel. 1991 Alginate synthesis by Pseudomonas aeruginosa: A key pathogenic factor in chronic pulmonary infections of cystic fibrosis patients Clin. Microbiol. Rev. 4 191–206

    CAS  PubMed  PubMed Central  Google Scholar 

  • McCaw, M. L., G. L. Lykken, P. K. Singh, and T. L. Yahr. 2002 ExsD is a negative regulator of the Pseudomonas aeruginosa type III secretion regulon Molec. Microbiol. 46 1123–1133

    CAS  Google Scholar 

  • Merz, A. J., M. So, and M. P. Sheetz. 2000 Pilus retraction powers bacterial twitching motility Nature 407 98–102

    CAS  PubMed  Google Scholar 

  • Morihara, K., and J. Y. Homma. 1985 Pseudomonas proteases In: A. Holder (Ed.) Bacterial Enzymes and Virulence CRC Press Boca Raton FL 41–79

    Google Scholar 

  • Nicas, T. I., and B. H. Iglewski. 1984 Isolation and characterization of transposon-induced mutants of Pseudomonas aeruginosa deficient in production of exoenzyme S Infect. Immun. 45 470–474

    CAS  PubMed  PubMed Central  Google Scholar 

  • Nicas, T. I., and B. H. Iglewski. 1985 The contribution of exoproducts to virulence of Pseudomonas aeruginosa Can. J. Microbiol. 31 387–392

    CAS  PubMed  Google Scholar 

  • Ogata, M., V. K. Chaudhary, I. Pastan, and D. J. FitzGerald. 1990 Processing of Pseudomonas exotoxin by a cellular protease results in the generation of a 37,000-Da toxin fragment that is translocated to the cytosol J. Biol. Chem. 265 20678–20685

    CAS  PubMed  Google Scholar 

  • Ostroff, R. M., B. Wretlind, and M. L. Vasil. 1989 Mutations in the hemolytic-phospholipase C operon result in decreased virulence of Pseudomonas aeruginosa PAO1 grown under phosphate-limiting conditions Infect. Immun. 57 1369–1373

    CAS  PubMed  PubMed Central  Google Scholar 

  • Ostroff, R. M., A. I. Vasil, and M. L. Vasil. 1990 Molecular comparison of a nonhemolytic and a hemolytic phospholipase C from Pseudomonas aeruginosa J. Bacteriol 172 5915–5923

    CAS  PubMed  PubMed Central  Google Scholar 

  • O’Toole, G. A., and R. Kolter. 1998 Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development Molec. Microbiol. 30 295–304

    Google Scholar 

  • Palleroni, N. J. 1984 Genus I. Pseudomonas Migula 1894 In: N. R. Kreig and J. G. Holt (Eds.) Bergey’s Manual of Systematic Bacteriology Williams and Wilkins Baltimore MA 141–199

    Google Scholar 

  • Parales, R. E., A. Ferrandez, and C. S. Harwood. 2004 Chemotaxis in Pseudomonas In: J.-L. Ramos (Ed.) Pseudomonas, Volume 1: Genomics, Lifestyle and Molecular Architecture Kluwer Academic/Plenum Publishers New York NY 793–815

    Google Scholar 

  • Parrillo, J. E., M. M. Parker, C. Natanson, A. F. Suffredini, R. L. Danner, R. E. Cunnion, and F. P. Ognibene. 1990 Septic shock in humans. Advances in the understanding of pathogenesis, cardiovascular dysfunction, and therapy Ann. Int. Med. 113 227–242

    CAS  PubMed  Google Scholar 

  • Parsek, M. R., D. L. Val, B. L. Hanzelka, J. E. Cronan Jr., and E. P. Greenberg. 1999 Acyl homoserine-lactone quorum-sensing signal generation Proc. Natl. Acad. Sci. USA 96 4360–4365

    CAS  PubMed  Google Scholar 

  • Parsek, M. R., and P. K. Singh. 2003 Bacterial biofilms: An emerging link to disease pathogenesis Ann. Rev. Microbiol. 57 677–701

    CAS  Google Scholar 

  • Passador, L., J. M. Cook, M. J. Gambello, L. Rust, and B. H. Iglewski. 1993 Expression of Pseudomonas aeruginosa virulence genes requires cell-to-cell communication Science 260 1127–1130

    CAS  PubMed  Google Scholar 

  • Pearson, J. P., K. M. Gray, L. Passador, K. D. Tucker, A. Eberhard, B. H. Iglewski, and E. P. Greenberg. 1994 Structure of the autoinducer required for expression of Pseudomonas aeruginosa virulence genes Proc. Natl. Acad. Sci. USA 91 197–201

    CAS  PubMed  Google Scholar 

  • Pearson, J. P., L. Passador, B. H. Iglewski, and E. P. Greenberg. 1995 A second N-acylhomoserine lactone signal produced by Pseudomonas aeruginosa Proc. Natl. Acad. Sci. USA 92 1490–1494

    CAS  PubMed  Google Scholar 

  • Pesci, E. C., J. P. Pearson, P. C. Seed, and B. H. Iglewski. 1997 Regulation of las and rhl quorum sensing in Pseudomonas aeruginosa J. Bacteriol 179 3127–3132

    CAS  PubMed  PubMed Central  Google Scholar 

  • Pesci, E. C., J. B. Milbank, J. P. Pearson, S. McKnight, A. S. Kende, E. P. Greenberg, and B. H. Iglewski. 1999 Quinolone signaling in the cell-to-cell communication system of Pseudomonas aeruginosa Proc. Natl. Acad. Sci. USA 96 11229–11234

    CAS  PubMed  Google Scholar 

  • Phillips, R. M., D. A. Six, E. A. Dennis, and P. Ghosh. 2003 In vivo phospholipase activity of the Pseudomonas aeruginosa cytotoxin ExoU and protection of mammalian cells with phospholipase A2 inhibitors J. Biol. Chem. 278 41326–41332

    CAS  PubMed  Google Scholar 

  • Pukatzki, S., R. H. Kessin, and J. J. Mekalanos. 2002 The human pathogen Pseudomonas aeruginosa utilizes conserved virulence pathways to infect the social amoeba Dictyostelium discoideum Proc. Natl. Acad. Sci. USA 99 3159–3164

    CAS  PubMed  Google Scholar 

  • Rahme, L. G., E. J. Stevens, S. F. Wolfort, J. Shao, R. G. Tompkins, and F. M. Ausubel. 1995 Common virulence factors for bacterial pathogenicity in plants and animals Science 268 1899–1902

    CAS  PubMed  Google Scholar 

  • Rahme, L. G., F. M. Ausubel, H. Cao, E. Drenkard, B. C. Goumnerov, G. W. Lau, S. Mahajan-Miklos, J. Plotnikova, M. W. Tan, J. Tsongalis, C. L. Walendziewicz, and R. G. Tompkins. 2000 Plants and animals share functionally common bacterial virulence factors Proc. Natl. Acad. Sci. USA 97 8815–8821

    CAS  PubMed  Google Scholar 

  • Rajan, S., and L. Saiman. 2002 Pulmonary infections in patients with cystic fibrosis Semin. Respir. Infect. 17 47–56

    PubMed  Google Scholar 

  • Reimmann, C., M. Beyeler, A. Latifi, H. Winteler, M. Foglino, A. Lazdunski, and D. Haas. 1997 The global activator GacA of Pseudomonas aeruginosa PAO positively controls the production of the autoinducer N-butyryl-homoserine lactone and the formation of the virulence factors pyocyanin, cyanide, and lipase Molec. Microbiol. 24 309–319

    CAS  Google Scholar 

  • Richards, M. J., J. R. Edwards, D. H. Culver, and R. P. Gaynes. 1999 Nosocomial infections in medical intensive care units in the United States: National Infections Surveillance System Crit. Care Med. 27 887–892

    CAS  PubMed  Google Scholar 

  • Roy-Burman, A., R. H. Savel, S. Racine, B. L. Swanson, N. S. Revadigar, J. Fujimoto, T. Sawa, D. W. Frank, and J. P. Wiener-Kronish. 2001 Type III protein secretion is associated with death in lower respiratory and systemic Pseudomonas aeruginosa infections J. Infect. Dis. 183 1767–1774

    CAS  PubMed  Google Scholar 

  • Saiman, L., and A. Prince. 1993 Pseudomonas aeruginosa pili bind to asialoGM1 which is increased on the surface of cystic fibrosis epithelial cells J. Clin. Invest. 92 1875–1880

    CAS  PubMed  PubMed Central  Google Scholar 

  • Sato, H., D. W. Frank, C. J. Hillard, J. B. Feix, R. R. Pankhaniya, K. Moriyama, V. Finck-Barbancon, A. Buchaklian, M. Lei, R. M. Long, J. Wiener-Kronish, and T. Sawa. 2003 The mechanism of action of the Pseudomonas aeruginosa-encoded type III cytotoxin, ExoU EMBO J. 22 2959–2969

    CAS  PubMed  PubMed Central  Google Scholar 

  • Sato, H., and D. W. Frank. 2004 ExoU is a potent intracellular phospholipase Molec. Microbiol. 53 1279–1290

    CAS  Google Scholar 

  • Sawa, T., T. L. Yahr, M. Ohara, K. Kurahashi, M. A. Gropper, J. P. Wiener-Kronish, and D. W. Frank. 1999 Active and passive immunization with the Pseudomonas V antigen protects against type III intoxication and lung injury Nature Med. 5 392–398

    CAS  PubMed  Google Scholar 

  • Schoehn, G., A. M. Di Guilmi, D. Lemaire, I. Attree, W. Weissenhorn, and A. Dessen. 2003 Oligomerization of type III secretion proteins PopB and PopD precedes pore formation in Pseudomonas EMBO J. 22 4957–4967

    CAS  PubMed  PubMed Central  Google Scholar 

  • Schuster, M., C. P. Lostroh, T. Ogi, and E. P. Greenberg. 2003 Identification, timing, and signal specificity of Pseudomonas aeruginosa quorum-controlled genes: A transcriptome analysis J. Bacteriol. 185 2066–2079

    CAS  PubMed  PubMed Central  Google Scholar 

  • Schuster, M., A. C. Hawkins, C. S. Harwood, and E. P. Greenberg. 2004 The Pseudomonas aeruginosa RpoS regulon and its relationship to quorum sensing Molec. Microbiol. 51 973–985

    CAS  Google Scholar 

  • Sheehan, D. J., J. M. Janda, and E. J. Bottone. 1982 Pseudomonas aeruginosa: Changes in antibiotic susceptibility, enzymatic activity, and antigenicity among colonial morphotypes J. Clin. Microbiol. 15 926–930

    CAS  PubMed  PubMed Central  Google Scholar 

  • Shibuya, Y., T. Yamamoto, T. Morimoto, N. Nishino, T. Kambara, and H. Okabe. 1991 Pseudomonas aeruginosa alkaline proteinase might share a biological function with plasmin Biochim. Biophys. Acta 1077 316–324

    CAS  PubMed  Google Scholar 

  • Shortridge, V. D., A. Lazdunski, and M. L. Vasil. 1992 Osmoprotectants and phosphate regulate expression of phospholipase C in Pseudomonas aeruginosa Molec. Microbiol. 6 863–871

    CAS  Google Scholar 

  • Stover, C. K., X. Q. Pham, A. L. Erwin, S. D. Mizoguchi, P. Warrener, M. J. Hickey, F. S. Brinkman, W. O. Hufnagle, D. J. Kowalik, M. Lagrou, R. L. Garber, L. Goltry, E. Tolentino, S. Westbrock-Wadman, Y. Yuan, L. L. Brody, S. N. Coulter, K. R. Folger, A. Kas, K. Larbig, R. Lim, K. Smith, D. Spencer, G. K. Wong, Z. Wu, I. T. Paulsen, J. Reizer, M. H. Saier, R. E. Hancock, S. Lory, and M. V. Olson. 2000 Complete genome sequence of Pseudomonas aeruginosa PA01, an opportunistic pathogen Nature 406 959–964

    CAS  PubMed  Google Scholar 

  • Sun, J., and J. T. Barbieri. 2003 Pseudomonas aeruginosa ExoT ADP-ribosylates CT10 regulator of kinase (Crk) proteins J. Biol. Chem. 278 32794–32800

    CAS  PubMed  Google Scholar 

  • Terada, L. S., K. A. Johansen, S. Nowbar, A. I. Vasil, and M. L. Vasil. 1999 Pseudomonas aeruginosa hemolytic phospholipase C suppresses neutrophil respiratory burst activity Infect. Immun. 67 2371–2376

    CAS  PubMed  PubMed Central  Google Scholar 

  • Thanassi, D. G., and S. J. Hultgren. 2000 Multiple pathways allow protein secretion across the bacterial outer membrane Curr. Opin. Cell Biol. 12 420–430

    CAS  PubMed  Google Scholar 

  • Torres, A., R. Aznar, J. M. Gatell, P. Jimenez, J. Gonzalez, A. Ferrer, R. Celis, and R. Rodriguez-Roisin. 1990 Incidence, risk, and prognosis factors of nosocomial pneumonia in mechanically ventilated patients Am. Rev. Respir. Dis. 142 523–528

    CAS  PubMed  Google Scholar 

  • Vallet, I., J. W. Olson, S. Lory, A. Lazdunski, and A. Filloux. 2001 The chaperone/usher pathways of Pseudomonas aeruginosa: Identification of fimbrial gene clusters (cup) and their involvement in biofilm formation Proc. Natl. Acad. Sci. USA 98 6911–6916

    CAS  PubMed  Google Scholar 

  • Vallet, I., S. P. Diggle, R. E. Stacey, M. Camara, I. Ventre, S. Lory, A. Lazdunski, P. Williams, and A. Filloux. 2004 Biofilm formation in Pseudomonas aeruginosa: Fimbrial cup gene clusters are controlled by the transcriptional regulator MvaT J. Bacteriol 186 2880–2890

    CAS  PubMed  PubMed Central  Google Scholar 

  • Vallis, A. J., T. L. Yahr, J. T. Barbieri, and D. W. Frank. 1999 Regulation of ExoS production and secretion by Pseudomonas aeruginosa in response to tissue culture conditions Infect. Immun. 67 914–920

    CAS  PubMed  PubMed Central  Google Scholar 

  • Vander Wauven, C., A. Pierard, M. Kley-Raymann, and D. Haas. 1984 Pseudomonas aeruginosa mutants affected in anaerobic growth on arginine: Evidence for a four-gene cluster encoding the arginine deiminase pathway J. Bacteriol 160 928–934

    CAS  PubMed  PubMed Central  Google Scholar 

  • Von Gotz, F., S. Haussler, D. Jordan, S. S. Saravanamuthu, D. Wehmhoner, A. Strussmann, J. Lauber, I. Attree, J. Buer, B. Tummler, and I. Steinmetz. 2004 Expression analysis of a highly adherent and cytotoxic small colony variant of Pseudomonas aeruginosa isolated from a lung of a patient with cystic fibrosis J. Bacteriol 186 3837–3847

    Google Scholar 

  • Voulhoux, R., G. Ball, B. Ize, M. L. Vasil, A. Lazdunski, L. F. Wu, and A. Filloux. 2001 Involvement of the twin-arginine translocation system in protein secretion via the type II pathway EMBO J. 20 6735–6741

    CAS  PubMed  PubMed Central  Google Scholar 

  • Wagner, V. E., D. Bushnell, L. Passador, A. I. Brooks, and B. H. Iglewski. 2003 Microarray analysis of Pseudomonas aeruginosa quorum-sensing regulons: Effects of growth phase and environment J. Bacteriol 185 2080–2095

    CAS  PubMed  PubMed Central  Google Scholar 

  • Wedekind, J. E., C. B. Trame, M. Dorywalska, P. Koehl, T. M. Raschke, M. McKee, D. FitzGerald, R. J. Collier, and D. B. McKay. 2001 Refined crystallographic structure of Pseudomonas aeruginosa exotoxin A and its implications for the molecular mechanism of toxicity J. Molec. Biol. 314 823–837

    CAS  PubMed  Google Scholar 

  • Wick, M. J., D. W. Frank, D. G. Storey, and B. H. Iglewski. 1990 Structure, function, and regulation of Pseudomonas aeruginosa exotoxin A Ann. Rev. Microbiol. 44 335–363

    CAS  Google Scholar 

  • Wiener-Kronish, J. P., T. Sakuma, I. Kudoh, J. F. Pittet, D. Frank, L. Dobbs, M. L. Vasil, and M. A. Matthay. 1993 Alveolar epithelial injury and pleural empyema in acute P. aeruginosa pneumonia in anesthetized rabbits J. Appl. Physiol. 75 1661–1669

    CAS  PubMed  Google Scholar 

  • Wolfgang, M. C., V. T. Lee, M. E. Gilmore, and S. Lory. 2003 Coordinate regulation of bacterial virulence genes by a novel adenylate cyclase-dependent signaling pathway Dev. Cell. 4 253–263

    CAS  PubMed  Google Scholar 

  • Yahr, T. L., J. T. Barbieri, and D. W. Frank. 1996a Genetic relationship between the 53-and 49-kilodalton forms of exoenzyme S from Pseudomonas aeruginosa J. Bacteriol 178 1412–1419

    CAS  PubMed  PubMed Central  Google Scholar 

  • Yahr, T. L., J. Goranson, and D. W. Frank. 1996b Exoenzyme S of Pseudomonas aeruginosa is secreted by a type III pathway Molec. Microbiol. 22 991–1003

    CAS  Google Scholar 

  • Yahr, T. L., A. J. Vallis, M. K. Hancock, J. T. Barbieri, and D. W. Frank. 1998 ExoY, an adenylate cyclase secreted by the Pseudomonas aeruginosa type III system Proc. Natl. Acad. Sci. USA 95 13899–13904

    CAS  PubMed  Google Scholar 

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Yahr, T.L., Parsek, M.R. (2006). Pseudomonas aeruginosa . In: Dworkin, M., Falkow, S., Rosenberg, E., Schleifer, KH., Stackebrandt, E. (eds) The Prokaryotes. Springer, New York, NY. https://doi.org/10.1007/0-387-30746-X_22

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