4. Conclusion
These studies demonstrate that it is possible to alter the carbohydrate recognition and viral neutralizing activities of SP-D through replacing the SP-D CRD with that of either conglutinin or MBL. These proteins have been useful tools in confirming that, 1. the enhanced ability of conglutinin to inhibit IAV infectivity as compared to SP-D results from differences in the CRDs of these proteins, and 2. the substantial greater viral aggregating and opsonizing activities of SP-D as compared to MBL result from differences in the N-terminal and collagen domains of these molecules. The chimeric collectins had significantly greater viral aggregating and neutralizing activity than either parent collectin from which they were derived. Hence, such constructs could be of therapeutic value in treatment of IAV infection. Although the two chimerae had similar activity against IAV, substantial differences were noted between their ability to bind to or aggregate bacteria (Hartshorn et al, unpublished data). Hence, there are probably significant differences between the functional properties of the CRD of conglutinin and that of MBL which need to be examined in further studies.
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Hartshorn, K.L., White, M.R., Ezekowitz, R.A.B., Sastry, K., Crouch, E. (2002). Development of Chimeric Collectins with Enhanced Activity Against Influenza a Virus. In: Keisari, Y., Ofek, I. (eds) The Biology and Pathology of Innate Immunity Mechanisms. Advances in Experimental Medicine and Biology, vol 479. Springer, Boston, MA. https://doi.org/10.1007/0-306-46831-X_5
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