Abstract
Culture broth of an actinomycete isolate, Nocardia sp. CS682 showed specifically higher antibacterial activity against methicilin resistant Staphylococcus aureus (MRSA). Purified substance from the organism, CS-682, which is active against MRSA and Micrococcus leuteus, is a C28H37NO8 (M+H+, observed: 516.83) and identified as an unusual macrolide antibiotic, nargenicin. The chemical structure of CS-682 was identified by FT-IR, 1H-NMR, 13C-NMR, and (1H-1H and 1H-13H) COSY. The anti-MRSA activity of CS-682 was stronger than that of oxacillin, vancomycin, monensin, erythromycin, and spiramycin. Phylogenetic analysis showed that strain CS682 is closely related to Nocardia tenerifensis DSM 44704T (98.7% sequence similarity), followed by N. brasiliensis ATCC 19296T (98.4% sequence similarity). The ability of Nocardia sp. CS682 to produce nargenicin was unique.
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Sohng, J.K., Yamaguchi, T., Seong, C.N. et al. Production, isolation and biological activity of nargenicin from Nocardia sp. CS682. Arch. Pharm. Res. 31, 1339–1345 (2008). https://doi.org/10.1007/s12272-001-2115-0
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DOI: https://doi.org/10.1007/s12272-001-2115-0