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Yeast diversity and species recovery rates from beech forest soils

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Abstract

Soil yeasts are globally diverse. They are found in almost all soil types, and the structure of soil yeast communities reflects aboveground vegetation properties. Cultivation techniques have often been successfully employed to study yeasts in forest soils. However, few studies have addressed the variation of soil yeast communities in space and time; especially, structural dynamics at a forest site between different seasons is unknown. Here, we analyse the results from our field experiments performed in 2008 and 2009. We reassess species inventory data and identify potential new species. Using improved species lists, we estimate the rate of species recovery from beech forest soils with a particular focus on repeated sampling. Our analyses showed that the number of observed yeast species was steadily increasing after one, two and three samplings. The observed diversity was likely approaching saturation after four samplings. Additionally, we provide formal descriptions of new yeast species isolated from forest soils in Germany during these studies, as 30 % of the observed species represented undescribed taxa. The following taxonomic novelties are proposed: Colacogloea demeterae Yurkov, Schäfer & Begerow sp. nov. (MB 816166), Slooffia velesii Federici, Röhl & Begerow sp. nov. (MB 816165), Hamamotoa cerberi Yurkov, Schäfer & Begerow sp. nov. (MB 816164), Hamamotoa telluris Yurkov, Schäfer & Begerow sp. nov. (MB 816163), Piskurozyma yama Richter, Mittelbach & Begerow, sp. nov. (MB 816162), Piskurozyma tuonelana Lotze-Engelhard, Richter & Begerow sp. nov. (MB 816161), Dioszegia dumuzii Ebinghaus, Prior & Begerow sp. nov. (MB 816160), and Chernovia houtui Federici, Yurkov & Begerow gen. nov. et sp. nov. (MB 816158, MB 816159).

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Acknowledgments

Ilse Weßel (Ruhr-University Bochum) is acknowledged for her assistance in the laboratory during cultivation experiments. Authors are grateful to the participants and collaborators of the DFG Biodiversity Exploratories for exchanging and sharing soil samples. We thank Hai D. T. Nguyen for improving the use of English in the manuscript. This work was funded by the DFG (Deutsche Forschungsgemeinschaft) Priority Program 1374 “Infrastructure-Biodiversity-Exploratories”, and the research grants from DFG (BE 2201/9-1; BE2201/16-1; YU152/1-1) and DAAD (A/07/94549). Field work permits were issued by the responsible state environmental offices of Baden-Württemberg and Thuringia (according to § 72 BbgNatSchG).

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Authors and Affiliations

  1. Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124, Braunschweig, Germany

    Andrey M. Yurkov

  2. Geobotany, Faculty of Biology and Biotechnology, Ruhr-University Bochum, 44801, Bochum, Germany

    Thorsten Wehde, Julian Federici, Angela M. Schäfer, Malte Ebinghaus, Sascha Lotze-Engelhard, Moritz Mittelbach, René Prior, Christian Richter, Oliver Röhl & Dominik Begerow

  3. Microbial Drugs, Helmholtz Centre for Infection Research, Inhoffenstraße 7, 38124, Braunschweig, Germany

    Christian Richter

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  1. Andrey M. Yurkov
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  2. Thorsten Wehde
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  3. Julian Federici
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  4. Angela M. Schäfer
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  5. Malte Ebinghaus
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  6. Sascha Lotze-Engelhard
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  7. Moritz Mittelbach
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  8. René Prior
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  9. Christian Richter
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  10. Oliver Röhl
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  11. Dominik Begerow
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Correspondence to Andrey M. Yurkov.

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Section Editor: Franz Oberwinkler

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Figure S1

Maximum likelihood analysis of an alignment of the ITS region for the genus Mrakia. The numbers provided on branches are frequencies (>50 %) with which a given branch appeared in 100 bootstrap replications. The scale bars indicate the numbers of expected substitutions accumulated per site. Type strains are designated with the symbol T. (GIF 101 kb)

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Yurkov, A.M., Wehde, T., Federici, J. et al. Yeast diversity and species recovery rates from beech forest soils. Mycol Progress 15, 845–859 (2016). https://doi.org/10.1007/s11557-016-1206-8

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  • DOI: https://doi.org/10.1007/s11557-016-1206-8

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