Abstract
Protein phosphatase 3 (PPP3, formerly PP2B, Calcineurin), a serine/ threonine protein phosphatase, is a heterodimer composed of one catalytic subunit (PPP3C, Calcineurin A) and one regulatory subunit (PPP3R, Calcineurin B). PPP3R, an EF-hand Ca2+ binding protein, contains four high-affinity EF-hand calcium-binding sites, indicating that PPP3 plays critical roles in many calcium-mediated signal transduction pathways. PPP3R has two isoforms, PPP3R1 (also known as PP2Bβ1) and PPP3R2 (also known as PP2Bβ2). While PPP3Rl is ubiquitously expressed in different tissues, PPP3R2 is exclusively expressed in testis. PPP3R2 has only been identified in rat and mouse. Here we report a human homologue of PPP3R2, which is designated PPP3RL (PPP3R like protein). PPP3RL gene was predicated to encode 171 amino acid residues with four EF-hand calcium-binding domains and this putative protein shares 82.9% and 80.5% identity with PPP3R2 of rat and mouse, respectively. Our results show that PPP3RL gene localizes to human chromosome 9q22 and transcripts of PPP3RL gene are specifically expressed in the testis, moreover, this tissue-specific expression is due to demethylation of its promoter region in testis.
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GD Plowman S Sudarsanam J Bingham D Whyte T Hunter (1999) Proc Natl. Acad. Sci. USA 96 13603–13610 Occurrence Handle1:CAS:528:DyaK1MXns1OqsL4%3D Occurrence Handle10570119
T Hunter (2000) Cell 100 113–127 Occurrence Handle1:CAS:528:DC%2BD3cXks1Crtg%3D%3D Occurrence Handle10647936
T Hunter (1995) Cell 80 225–236 Occurrence Handle1:CAS:528:DyaK2MXjtlGnsrs%3D Occurrence Handle7834742
F Rusnak P Mertz (2000) Phys. Rev. 80 1483–1521 Occurrence Handle1:CAS:528:DC%2BD3cXnsVKisLk%3D
BL Stoddard KE Flick (1996) Curr. Opin. Struct. Biol. 6 770–775 Occurrence Handle1:CAS:528:DyaK2sXhtlGmuw%3D%3D Occurrence Handle8994877
S Baksh SJ Burakoff (2000) Semin Immunol. 12 405–415 Occurrence Handle1:CAS:528:DC%2BD3cXmsFKltr4%3D Occurrence Handle10995587
D Guerini (1997) Biochem. Biophys. Res. Commun. 235 271–275 Occurrence Handle1:CAS:528:DyaK2sXktFSiu7k%3D Occurrence Handle9199180
CB Klee H Ren X Wang (1998) J. Biol. Chem. 273 13367–13370 Occurrence Handle1:CAS:528:DyaK1cXjvVejsLs%3D Occurrence Handle9593662
B Feng PM Stemmer (1999) Biochemistry 38 12481–12489 Occurrence Handle1:CAS:528:DyaK1MXltlyqtL4%3D Occurrence Handle10493818
MG Wang H Yi D Guerini CB Klee OW McBride (1996) Cytogenet. Cell Genet. 72 236–241 Occurrence Handle1:CAS:528:DyaK28XitVyrtLc%3D Occurrence Handle8978785
T Muramatsu RL Kinacaid (1992) Biochem. Biophys. Res. Commun. 188 265–271 Occurrence Handle1:CAS:528:DyaK3sXkvVOltbc%3D Occurrence Handle1339277
D Guerini MH Krinks JM Sikela WE Hahn CB Klee (1989) DNA 8 675–682 Occurrence Handle1:CAS:528:DyaK3cXktVWgtr8%3D Occurrence Handle2558868
H Mukai CD Chang H Tanaka A Jto T Kuno C Tanaka (1991) Biochem. Biophys. Res. Commun. 179 1325–1330 Occurrence Handle1:CAS:528:DyaK38Xlt1Gltrg%3D Occurrence Handle1718268
K Miyamoto H Matsui K Tomizawa Y Kuwata T Itano M Tokuda O Hatase (1994) Biochem. Biophys. Res. Commun. 203 1275–1283 Occurrence Handle1:CAS:528:DyaK2cXmtVWmtL0%3D Occurrence Handle8093044
CD Chang H Mukai T Kuno C Tanaka (1994) Biochem. Biophys. Acta 1217 174–180 Occurrence Handle1:CAS:528:DyaK2cXisVSqur0%3D Occurrence Handle8110831
Q Su M Zhao E Weber H Eugster B Ryffel (1995) Eur. J. Biochem. 230 469–474 Occurrence Handle1:CAS:528:DyaK2MXmtlSqs7o%3D Occurrence Handle7607217
K Ueki T Muramatsu RL Kincaid (1992) Biochem. Biophys. Res. Commun. 187 537–543 Occurrence Handle1:CAS:528:DyaK3sXks1Kgu7w%3D Occurrence Handle1325794
J Zhang L Yu Q Fu J Gao Y Xie J Chen P Zhang Q Liu S Zhao (2001) Gene 264 273–279 Occurrence Handle1:CAS:528:DC%2BD3MXhsleks7k%3D Occurrence Handle11250083
M Ariel J Mccarreyt H Cedar (1991) Proc. Natl. Acad. Sci. USA 88 2317–2321 Occurrence Handle1:CAS:528:DyaK3MXhsFygu7s%3D Occurrence Handle2006171
C Grunau W Hindermann A Rosenthal (2000) Hum. Mol. Genet. 9 2651–2663 Occurrence Handle1:CAS:528:DC%2BD3cXosVKktLc%3D Occurrence Handle11063724
IC Yang SK Yang CW Park JH Chung (2000) Biochem. Biophys. Res. Commun. 274 722–726 Occurrence Handle1:CAS:528:DC%2BD3cXltlert7s%3D Occurrence Handle10924343
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Liu, L., Zhang, J., Yuan, J. et al. Characterization of a human regulatory subunit of protein phosphatase 3 gene (PPP3RL) expressed specifically in testis. Mol Biol Rep 32, 41–45 (2005). https://doi.org/10.1007/s11033-004-4250-4
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DOI: https://doi.org/10.1007/s11033-004-4250-4