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The reduction effect and mechanism of Deinococcus radiodurans transformed dsrA gene to uranyl ions

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Abstract

Deinococcus radiodurans (DR) is highly resistant to ionizing radiation. This study aims to convert dsrA gene into DR to construct radiation-resistant genetically engineered bacteria (Deino-dsrA) with high reducibility, so as to enhance the reducing and enrichment ability of DR. Methods: the recombinant vector pRADK-dsrA was extracted and transformed into DR. 1. Radiation resistant gene engineering bacteria containing dsrA gene were constructed successfully. 2. In the most favorable conditions,contributing to approximately 92.45% U (VI) was removed. 3. Autioxidant enzyme activities in Deino-dsrA is higher than in Deino-pRADK, excepting the content of malondialdehyde. The uranium enrichment ability of the Deino-dsrA is better than the wild DR, and dsrA gene can increase its antioxidation capability by increasing the activity of oxidase.

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Acknowledgements

This work was financially supported by the Nature Science Foundation of Hunan Province in 2020(2020JJ6050、2020JJ4077).

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  1. Conghui Cheng and Jingxi Xie have contributed equally to this work and should be considered co-first authors

Authors and Affiliations

  1. School of Public Health, University of South China, Hengyang, 421001, Hunan, People’s Republic of China

    Conghui Cheng, Qiqi Zhu, Luyao Chen, Kexin Guo, Shanshan Li, Shuya He & Fangzhu Xiao

  2. School of Chemistry and Chemical Engineering, University of South China, Hengyang, 421001, Hunan, People’s Republic of China

    Jingxi Xie

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  1. Conghui Cheng
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Cheng, C., Xie, J., Zhu, Q. et al. The reduction effect and mechanism of Deinococcus radiodurans transformed dsrA gene to uranyl ions. J Radioanal Nucl Chem 330, 1075–1090 (2021). https://doi.org/10.1007/s10967-021-08038-7

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  • DOI: https://doi.org/10.1007/s10967-021-08038-7

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