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Cohnella candidum sp. nov., radiation-resistant bacterium from soil

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Abstract

A Gram-stain positive, endospore-forming, circular, convex, cream colored, designated strain 18JY8-7T, was isolated from soil collected in Jeju Island, South Korea. Phylogenetic analysis using 16S rRNA gene sequences showed that strain 18JY8-7T formed a distinct lineage within the family Paenibacillaceae (order Bacillales, class Bacilli), and is closely related to Cohnella rhizosphaerae (96.1%, sequence similarity) and Cohnella xylanilytica (96.0%). Optimal growth occurred at 30 °C, pH 6.5 and in the absence of NaCl. The predominant cellular fatty acids were anteiso-C15:0 and iso-C16:0. The major respiratory quinone was MK-7. The polar lipids profile comprised of diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. The DNA G + C content was 57.0 mol %. The genotypic and phenotypic analyses revealed the differentiation of strain 18JY8-7T from all recognized Cohenella species. The strain 18JY8-7T, therefore represents a novel bacterial species within the family Paenibacillaceae, for which the name Cohnella candidum sp. nov. is proposed. The type strain is 18JY8-7T (= KCTC 33969T = JCM 33199T).

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Acknowledgements

This work was supported by a research grant from Seoul Women’s University (2018) and by a Grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR201801106).

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Authors and Affiliations

  1. Department of Public Health Sciences, Graduate School, Korea University, Seoul, 02841, Republic of Korea

    SooHyun Maeng & Hana Yi

  2. Department of Bio and Environmental Technology, College of Natural Science, Seoul Women’s University, Seoul, 01797, Republic of Korea

    Myung Kyum Kim & Gayathri Subramani

  3. Department of Horticultural Science, School of Applied Biosciences, Institute of Plant Medicine, Kyungpook National University, Daegu, 41566, Republic of Korea

    Jun Hwee Jang

  4. School of Biosystem and Biomedical Science, Korea University, Seoul, 02841, Republic of Korea

    Hana Yi

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Maeng, S., Kim, M.K., Jang, J.H. et al. Cohnella candidum sp. nov., radiation-resistant bacterium from soil. Antonie van Leeuwenhoek 112, 1029–1037 (2019). https://doi.org/10.1007/s10482-019-01233-3

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