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Acidovorax lacteus sp. nov., isolated from a culture of a bloom-forming cyanobacterium (Microcystis sp.)

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Abstract

A novel Gram-negative, rod-shaped and motile bacterial strain, designated strain M36T, was isolated from a culture of a bloom-forming cyanobacterium, Microcystis sp., collected from a eutrophic lake in Korea. Its taxonomic position was investigated by using a polyphasic taxonomic approach. The isolate was found to grow aerobically at 15–42 °C (optimum 25 °C), pH 7.0–11.0 (optimum pH 8.0) and in the presence of 0–1.0% (w/v) NaCl (optimum 0% NaCl) on R2A medium. The phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain M36T is closely related to Acidovorax anthurii DSM 16745T (98.1%), Acidovorax konjaci DSM 7481T (97.7%) and Acidovorax avenae DSM 7227T (97.0%) and also formed a clear phylogenetic lineage with other Acidovorax species. DNA–DNA relatedness between strain M36T and the closely related species of the genus Acidovorax was <30%. The major fatty acid components identified included summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), C16:0 and summed feature 8 (C18:0 ω7c and/or C18:0 ω6c). The DNA G+C content of strain M36T was determined to be 66.8 mol%. Based on above polyphasic evidence, strain M36T is concluded to represent a new species of genus Acidovorax, for which the name Acidovorax lacteus sp. nov. is proposed. The type strain is M36T (=KCTC 52220T = JCM 31890T).

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Acknowledgements

This research was supported by the Basic Core Technology Development Program for the Oceans and the Polar Regions of the National Research Foundation (NRF) funded by the Ministry of Science, ICT and Future Planning (2016M1A5A1027453). This work was supported by the KRIBB Research Initiative Program.

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Authors and Affiliations

  1. Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea

    Seong-Jun Chun, Yingshun Cui, So-Ra Ko, Hyung-Gwan Lee, Ankita Srivastava, Hee-Mock Oh & Chi-Yong Ahn

  2. Department of Environmental Biotechnology, KRIBB School of Biotechnology, Korea University of Science and Technology(UST), 217 Gajeong-ro, Yuseong-gu, Daejeon, 34113, Republic of Korea

    Seong-Jun Chun, Hee-Mock Oh & Chi-Yong Ahn

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  1. Seong-Jun Chun
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Correspondence to Chi-Yong Ahn.

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Chun, SJ., Cui, Y., Ko, SR. et al. Acidovorax lacteus sp. nov., isolated from a culture of a bloom-forming cyanobacterium (Microcystis sp.). Antonie van Leeuwenhoek 110, 1199–1205 (2017). https://doi.org/10.1007/s10482-017-0892-9

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