Abstract
A Gram-negative, rod-shaped, non-motile, non-spore forming, aerobic, orange-pigmented bacterium, designated strain 6PT, was isolated from a soil sample collected from the Hoh Xil basin, China. Strain 6PT grew optimally at 25 °C, pH 7.0–7.5 and NaCl concentration of 0–1 % (w/v). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 6PT belongs to the genus Sphingomonas, with high sequence similarity (97.1 %) to Sphingomonas fennica. The DNA–DNA hybridization homology with S. fennica DSM 13665T was 45.3 %. The DNA G+C content of the novel strain is 65.3 mol%. The isolate contained Q-10 as the only respiratory quinone. The major polar lipids are diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylcholine (PC) and sphingoglycolipid (SGL). C18:1 ω7c and C16:1 ω7c are the major fatty acids. On the basis of the polyphasic evidence presented, strain 6PT represents a novel species of the genus Sphingomonas, for which the name Sphingomonas arantia sp. nov. is proposed. The type strain is 6PT (=CGMCC 1.12702T = JCM 19855T).
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Acknowledgments
This research is financed by the National Special Research Fund (Gas Hydrate Resource Exploration and Production Testing Project, Grant No.: GZHL20110308, GZHL20110323). We thank supporters of our lab and also the identification service of the China General Microbiological Culture Collection Center (CGMCC) for their help. Specially, we thank Prof. Bernhard Schink for the help of the guidance about naming the new strain.
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Jia, L., Zheng, Z., Feng, X. et al. Sphingomonas arantia sp. nov., isolated from Hoh Xil basin, China. Antonie van Leeuwenhoek 108, 1341–1347 (2015). https://doi.org/10.1007/s10482-015-0586-0
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DOI: https://doi.org/10.1007/s10482-015-0586-0