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Actibacterium atlanticum sp. nov., isolated from surface seawater of the Atlantic Ocean

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Abstract

A taxonomic study was carried out on strain 22II-S11-z10T, which was isolated from the surface seawater of the Atlantic Ocean. The bacterium was found to be Gram-stain negative, oxidase and catalase positive, oval- to rod-shaped and non-motile. Growth was observed at salinities of 0.5–9 % and at temperatures of 10–41 °C. The isolate can reduce nitrate to nitrite, degrade gelatin and aesculin, but can not degrade Tween 80. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 22II-S11-z10T belongs to the genus Actibacterium, with the highest sequence similarity to the type strain Actibacterium mucosum CECT 7668T (97.3 %). The DNA–DNA hybridization estimate value between strain 22II-S11-z10T and A. mucosum CECT 7668T was 19.30 ± 2.29 %. The principal fatty acids were identified as Summed Feature 8 (C18:1 ω7c/ω6c as defined by the MIDI system, 75.2 %) and Summed Feature 3 (C16:1 ω7c/ω6c, 6.9 %). The G+C content of the chromosomal DNA was determined to be 59.0 mol%. The respiratory quinone was determined to be Q-10 (100 %). Phosphatidylglycerol, phosphatidylcholine, two phospholipids, two aminolipids and two lipids were identified in the polar lipids. The combined genotypic and phenotypic data show that strain 22II-S11-z10T represents a novel species within the genus Actibacterium, for which the name Actibacterium atlanticum sp. nov. is proposed, with the type strain 22II-S11-z10T (=MCCC 1A09298T = LMG 27158T).

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Abbreviations

MCCC:

Marine Culture Collection of China

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Acknowledgments

This work was financially supported by COMRA program (No. DY125-15-R-01), Public Welfare Project of SOA (201005032) and National Infrastructure of Natural Resources for Science and Technology Program of China (No. NIMR-2013-9).

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Authors and Affiliations

  1. State Key Laboratory Breeding Base of Marine Genetic Resources, Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA, Key Laboratory of Marine Genetic Resources of Fujian Province, Collaborative Innovation Center of Deep Sea Biology, Xiamen, 361005, China

    Guizhen Li, Qiliang Lai, Fengqin Sun, Yaping Du, Xiupian Liu, Guangyu Li, Yunbiao Xie & Zongze Shao

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  1. Guizhen Li
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  2. Qiliang Lai
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  3. Fengqin Sun
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  5. Xiupian Liu
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  6. Guangyu Li
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  7. Yunbiao Xie
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Corresponding author

Correspondence to Zongze Shao.

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Guizhen Li and Qiliang Lai contributed equally to this work.

Transmission electron micrographs of cells of strain 22II-S11-z10T, polar lipids of strain 22II-S11-z10T, Maximum-Likelihood tree and Minimum Evolution tree are available as supplementary figures in ANTO Online.

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Li, G., Lai, Q., Sun, F. et al. Actibacterium atlanticum sp. nov., isolated from surface seawater of the Atlantic Ocean. Antonie van Leeuwenhoek 106, 325–330 (2014). https://doi.org/10.1007/s10482-014-0203-7

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