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Roseovarius gaetbuli sp. nov., a novel alphaproteobacterium isolated from a tidal flat sediment

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Abstract

A Gram-negative, aerobic, non-motile and ovoid or rod-shaped bacterial strain, designated YM-20T, was isolated from a tidal flat of the Yellow Sea in South Korea. Strain YM-20T was found to grow optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain YM-20T clustered consistently with the type strains of ‘Roseovarius marisflavi’ and Roseovarius lutimaris, with which it exhibited 16S rRNA gene sequence similarities of 99.86 and 98.71 %, respectively. Strain YM-20T was found to contain Q-10 as the predominant ubiquinone and C18:1 ω7c as the predominant fatty acid. The major polar lipids of strain YM-20T were identified as phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain YM-20T was determined to be 60.9 mol% and its DNA–DNA relatedness values with the type strains of ‘R. marisflavi’, R. lutimaris and Pelagicola litorisediminis were 53 ± 7.1, 22 ± 5.5 and 13 ± 4.7  %, respectively. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain YM-20T is separated from ‘R. marisflavi’, R. lutimaris, the other Roseovarius species and P. litorisediminis. On the basis of the data presented, strain YM-20T is considered to represent a novel species of the genus Roseovarius, for which the name Roseovarius gaetbuli sp. nov. is proposed. The type strain is YM-20T (= KCTC 32428T = CECT 8370T).

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Acknowledgments

This work was supported by a Grant from the Program for Collection, Management and Utilization of Biological Resources (Grant NRF-Choi et al. 2013M3A9A5075953) from the Ministry of Science, ICT & Future Planning (MSIP) of the Republic of Korea. We are grateful to Prof. Che Ok Jeon for kindly providing Roseovarius lutimaris 112T.

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Authors and Affiliations

  1. Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea

    Sooyeon Park, Ja-Min Park, Chul-Hyung Kang & Jung-Hoon Yoon

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  1. Sooyeon Park
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  2. Ja-Min Park
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  3. Chul-Hyung Kang
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  4. Jung-Hoon Yoon
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Correspondence to Jung-Hoon Yoon.

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The TLC of the total polar lipids of strain YM-20T is available as a supplementary figure with the online version of this paper.

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Park, S., Park, JM., Kang, CH. et al. Roseovarius gaetbuli sp. nov., a novel alphaproteobacterium isolated from a tidal flat sediment. Antonie van Leeuwenhoek 105, 723–730 (2014). https://doi.org/10.1007/s10482-014-0127-2

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  • DOI: https://doi.org/10.1007/s10482-014-0127-2

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