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Low-level laser therapy improves the inflammatory profile of rats with heart failure

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Abstract

Following heart failure (HF), immune activation leads to an imbalance between pro-inflammatory and anti-inflammatory cytokines. Low-level laser therapy (LLLT) has been used as an anti-inflammatory treatment in several disease conditions. However, the effect of LLLT on the skeletal muscle of rats with HF remains unclear. The present report aimed to evaluate the influence of LLLT on the inflammatory profile of rats with HF. The left coronary artery was ligated to induce HF and a sham operation was performed in the control groups. Male Wistar rats (n = 49) were assigned to one of six groups: placebo sham rats (P-Sham; n = 8), LLLT at a dose of 3 J/cm2 sham rats (3 J/cm2-Sham; n = 8), LLLT at a dose of 21 J/cm2 sham rats (21 J/cm2-Sham; n = 8), placebo HF rats (P-HF; n = 9), LLLT at a dose of 3 J/cm2 HF rats (3 J/cm2-HF; n = 8), and LLLT at a dose of 21 J/cm2 HF rats (21 J/cm2-HF; n = 8). Four weeks after myocardial infarction or sham surgery, rats were subjected to LLLT (InGaAlP 660 nm, spot size 0.035 cm2, output power 20 mW, power density 0.571 W/cm2, energy density 3 or 21 J/cm2, exposure time 5.25 s and 36.75 s) on the right gastrocnemius for 10 consecutive days. LLLT reduced plasma IL-6 levels (61.3 %; P < 0.01), TNF-α/IL-10 (61.0 %; P < 0.01) and IL-6/IL-10 ratios (77.3 %; P < 0.001) and increased IL-10 levels (103 %; P < 0.05) in the 21 J/cm2-HF group. Moreover, LLLT reduced the TNF-α (20.1 % and 21.3 %; both P < 0.05) and IL-6 levels (54.3 % and 37.8 %; P < 0.01 and P < 0.05, respectively) and the IL-6/IL-10 ratio (59.7 % and 42.2 %; P < 0.001 and P < 0.05, respectively) and increased IL-10 levels (81.0 % and 85.1 %; both P < 0.05) and the IL-10/TNF-α ratio (171.5 % and 119.8 %; P < 0.001 and P < 0.05, respectively) in the gastrocnemius in the 3 J/cm2-HF and 21 J/cm2-HF groups. LLLT showed systemic and skeletal muscle anti-inflammatory effects in rats with HF.

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Acknowledgments

We are thankful to Prof. Emerson Soldadeli Boschi, Tiago Heck MSc., Dr. Eurico Camargo Neto and André Costa for their support during the development of this study. This work was supported by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brasília, Brazil. PROAP/UFCSPA.

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Authors and Affiliations

  1. Laboratório de Fisiologia, Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Porto Alegre, RS, Brazil

    Vítor S. Hentschke, Rodrigo B. Jaenisch, Letiane A. Schmeing, Paulo R. Cavinato & Pedro Dal Lago

  2. Laboratório de Biologia Celular e Tecidual, Departamento de Ciências Morfofisiológicas, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil

    Leder L. Xavier

  3. Programa de Pós-Graduação em Ciências da Reabilitação, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Porto Alegre, RS, Brazil

    Pedro Dal Lago

  4. Physical Therapy Department, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Rua Sarmento Leite, 245, 90050-170, Porto Alegre, RS, Brazil

    Pedro Dal Lago

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  1. Vítor S. Hentschke
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Hentschke, V.S., Jaenisch, R.B., Schmeing, L.A. et al. Low-level laser therapy improves the inflammatory profile of rats with heart failure. Lasers Med Sci 28, 1007–1016 (2013). https://doi.org/10.1007/s10103-012-1190-4

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