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Characterization of Halanaerobaculum tunisiense gen. nov., sp. nov., a new halophilic fermentative, strictly anaerobic bacterium isolated from a hypersaline lake in Tunisia

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Abstract

A new halophilic anaerobe was isolated from the hypersaline surface sediments of El-Djerid Chott, Tunisia. The isolate, designated as strain 6SANG, grew at NaCl concentrations ranging from 14 to 30%, with an optimum at 20–22%. Strain 6SANG was a non-spore-forming, non-motile, rod-shaped bacterium, appearing singly, in pairs, or occasionally as long chains (0.7–1 × 4–13 μm) and showed a Gram-negative-like cell wall pattern. It grew optimally at pH values between 7.2 and 7.4, but had a very broad pH range for growth (5.9–8.4). Optimum temperature for growth was 42°C (range 30–50°C). Strain 6SANG required yeast extract for growth on sugars. Glucose, sucrose, galactose, mannose, maltose, cellobiose, pyruvate, and starch were fermented. The end products from glucose fermentation were acetate, butyrate, lactate, H2, and CO2. The G + C ratio of the DNA was 34.3 mol%. Strain 6SANG exhibited 16S rRNA gene sequence similarity values of 91–92% with members of the genus Halobacteroides, H. halobius being its closest phylogenetic relative. Based on phenotypic and phylogenetic characteristics, we propose that this bacterium be classified as a novel species of a novel genus, Halanaerobaculum tunisiense gen. nov., sp. nov. The type strain is 6SANGT (=DSM 19997T = JCM 15060T).

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Acknowledgments

We thank French university cooperation and the Tunisian Ministry for Scientific Technology and Development for their financial assistance (CMCU) and Pierre Thomas for help with electron microscopy. We thank also Mhamed Ben Abid for help in the physico-chemical analysis.

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Authors and Affiliations

  1. Laboratoire Microorganismes et Biomolécules Actives, Faculté des Sciences de Tunis, Université de Tunis El Manar, 2092, Tunis, Tunisia

    Abdeljabbar Hedi, Najla Sadfi & Abdellatif Boudabous

  2. Laboratoire de Microbiologie et de Biotechnologie des Environnements Chauds, UMR 180, IRD, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL, case 925, 13288, Marseille Cedex 9, France

    Abdeljabbar Hedi, Marie-Laure Fardeau, Bernard Ollivier & Jean-Luc Cayol

Authors
  1. Abdeljabbar Hedi
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  2. Marie-Laure Fardeau
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  3. Najla Sadfi
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  4. Abdellatif Boudabous
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  5. Bernard Ollivier
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  6. Jean-Luc Cayol
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Correspondence to Jean-Luc Cayol.

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Communicated by H. Santos.

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Hedi, A., Fardeau, ML., Sadfi, N. et al. Characterization of Halanaerobaculum tunisiense gen. nov., sp. nov., a new halophilic fermentative, strictly anaerobic bacterium isolated from a hypersaline lake in Tunisia. Extremophiles 13, 313–319 (2009). https://doi.org/10.1007/s00792-008-0218-y

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  • DOI: https://doi.org/10.1007/s00792-008-0218-y

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