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Molecular phylogeny of Shortia sensu lato (Diapensiaceae) based on multiple nuclear sequences

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Abstract

Molecular phylogenetics is commonly used to perform accurate taxonomic classification. Furthermore, nuclear multilocus sequences have been applied to resolve ambiguous phylogenetic relationships based on conventional markers such as chloroplast and nuclear ribosomal DNA. Here, we reconstructed the phylogenetic tree of Diapensiaceae to revise the traditional classification of the genus Shortia sensu lato (s.l.), which includes Shortia sensu stricto (s.s.) and Schizocodon; whether the latter taxon is treated as an independent genus or includes Shortia s.l. has been disputed since its first description. The phylogeny based on eight nuclear markers developed for this study indicated that Shortia s.l. is not a monophyletic group and that Schizocodon and Shortia s.s. are reciprocally monophyletic. In addition, Schizocodon would be sister to other genus Diapensia despite their morphological differentiation. Thus, our data suggest that Schizocodon represents an independent genus. Our study demonstrated that multiple nuclear loci can resolve obscure phylogenetic relationships determined using conventional markers.

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Acknowledgments

We thank T. Ohtsuki, N. Ishibashi, H. Oh, N. Ohki, S. Yamada, H. Yagi, T. Wakabayashi, H. Imogawa, S. Nozoe, Q. Han, D. Takahashi and Y. Umetsu (Kyoto University) for helpful advice regarding the experiments. This study was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology, Japan (21370032 and 22405013 to HS) and a Grant-in-Aid for JSPS Fellows (HH).

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Authors and Affiliations

  1. Graduate School of Human and Environmental Studies, Kyoto University, Yoshida-nihonmatsu-cho, Sakyo-ku, Kyoto, 606-8501, Japan

    Hiroyuki Higashi & Hiroaki Setoguchi

  2. Institute of Plant Science and Resources, Okayama University, Chuo 2-20-1, Kurashiki, 710-0046, Okayama, Japan

    Hajime Ikeda

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  1. Hiroyuki Higashi
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  2. Hajime Ikeda
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  3. Hiroaki Setoguchi
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Correspondence to Hiroyuki Higashi.

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Higashi, H., Ikeda, H. & Setoguchi, H. Molecular phylogeny of Shortia sensu lato (Diapensiaceae) based on multiple nuclear sequences. Plant Syst Evol 301, 523–529 (2015). https://doi.org/10.1007/s00606-014-1088-7

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  • DOI: https://doi.org/10.1007/s00606-014-1088-7

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