Skip to main content

Advertisement

SpringerLink
Log in

Stimulation of type II collagen biosynthesis and secretion in bovine chondrocytes cultured with degraded collagen

  • Regular Article
  • Published:
Cell and Tissue Research Aims and scope Submit manuscript

Abstract

The functional integrity of articular cartilage is dependent on the maintenance of the extracellular matrix (ECM), a process which is controlled by chondrocytes. The regulation of ECM biosynthesis is complex and a variety of substances have been found to influence chondrocyte metabolism. In the present study we have investigated the effect of degraded collagen on the formation of type II collagen by mature bovine chondrocytes in a cell culture model. The culture medium was supplemented with collagen hydrolysate (CH) and biosynthesis of type II collagen by chondrocytes was compared to control cells treated with native type I and type II collagen and a collagen-free protein hydrolysate. The quantification of type II collagen by means of an ELISA technique was confirmed by immunocytochemical detection as well as by the incorporation of 14C-proline in the ECM after a 48 h incubation. Chondrocytes in the control group were maintained in the basal medium for 11 days. The presence of extracellular CH led to a dose-dependent increase in type II collagen secretion. However, native collagens as well as a collagen-free hydrolysate of wheat proteins failed to stimulate the production of type II collagen in chondrocytes. These results clearly indicate a stimulatory effect of degraded collagen on the type II collagen biosynthesis of chondrocytes and suggest a possible feedback mechanism for the regulation of collagen turnover in cartilage tissue.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4a, b.
Fig. 5.
Fig. 6.

Similar content being viewed by others

References

  • Adyelotte MB, Schmid TR, Greenhill RR, Luchene L, Schumacher BL, Kuettner KE (1991) Synthesis of collagen by cultured bovine chondrocytes derived from different depth of articular cartilage. Trans Orthop Res Soc 16:26

    Google Scholar 

  • Aigner T, Glückert K, von der Mark K (1997) Activation of fibrillar collagen synthesis and phenotypic modulation of chondrocytes in early human osteoarthritic lesions. Osteoarthritis Cartilage 5:183–189

    Google Scholar 

  • Aycock RS, Raghow R, Stricklin GP, Seyer JM, Kang AH (1986) Post-transcriptional inhibition of collagen and fibronectin synthesis by a synthetic homolog of a portion of the carboxyl-terminal propeotide of human type I collagen. J Biol Chem 261:14355–14360

    Google Scholar 

  • Bateman JF, Lamandé SR, Ramshaw JAM (1996) Collagen superfamily. In: Comper WD (ed) Extracellular matrix, vol 2. Harwood Academic, Amsterdam, pp 22–67

  • Benya PD, Padilla SR, Nimni ME (1987) Independent regulation of collagen types by chondrocytes during the loss of differentiated function in culture. Cell 15:1313–1321

    Google Scholar 

  • Benz K, Breit S, Lukoschek M, Mau H, Richter W (2002) Molecular analysis of expansion, differentiation, and growth factor treatment of human chondrocytes identifies differentiation markers and growth-related genes. Biochem Biophys Res Commun 293:284–292

    Google Scholar 

  • Buckwalter JA, Mankin HJ (1998) Articular cartilage: degeneration and osteoarthritis, repair, regeneration, and transplantation. Instr Course Lect 47:487–504

    Google Scholar 

  • Burstein D, Bashir A, Gray ML (2000) MRI techniques in early stages of cartilage disease. Invest Radiol 35:602–621

    Google Scholar 

  • Elima K, Vuorio E (1989) Expression of mRNAs for collagen and other matrix components in dedifferentiating and redifferentiating human chondrocytes in culture. FEBS Lett 258:195–198

    Google Scholar 

  • Eyre DR, Wu JJ, Woods PE (1991) The cartilage collagens: structural and metabolic studies. J Rheumatol 18:49–51

    Google Scholar 

  • Hardingham TE, Bayliss M (1990) Proteoglycans of articular cartilage: changes in aging and in joint disease. Semin Arthritis Rheum 3:12–33

    Google Scholar 

  • Huber M, Tratting S, Lintner F (2000) Anatomy, biochemistry, and physiology of articular cartilage. Invest Radiol 35:573–580

    Google Scholar 

  • Katayama K, Seyer JM, Raghow R, Kang AH (1991) Regulation of extracellular matrix production by chemically synthesized subfragments of type I collagen carboxy propeptide. Biochemistry 30:7097–7104

    Google Scholar 

  • Lotz M, Blanco FJ, von Kempis J, et al. (1995) Cytokine regulation of chondrocyte functions. J Rheumatol 43:104–108

    Google Scholar 

  • Mayne R (1989) Collagens: what is their function, are they involved in articular disease. Arthritis Rheum 32:241–246

    Google Scholar 

  • Mlynarik V, Tratting S (2000) Physicochemical properties of normal articular cartilage and its MR appearance. Invest Radiol 35:589–594

    Google Scholar 

  • Moskowitz RW (2000) Role of collagen hydrolysate in bone and joint disease. Semin Arthritis Rheum 30:87–99

    Google Scholar 

  • Muir H (1995) The chondrocyte, architect of cartilage: biomechanics, structure, function and molecular biology of cartilage matrix macromolecules. Bioessays 17:1039–1048

    Google Scholar 

  • Oesser S, Adam M, Babel W, Seifert J (1999) Oral administration of 14C labeled gelatin hydrolysate leads to an accumulation of radioactivity in cartilage of mice (C57/BL). J Nutr 129:1891–1895

    Google Scholar 

  • Osborne KD, Trppel SB, Mankin HJ (1989) Growth factor stimulation of adult articular cartilage. J Orthop Res 7:35–42

    Google Scholar 

  • Pasternak RD, Hubbs SJ, Caccese RG, Marks RL, Conaty JM, DiPasquale G (1986) Interleukin-1 stimulates the secretion of proteoglycan- and collagen-degrading proteases by rabbit articular chondrocytes. Clin Immunol 41:351–367

    Google Scholar 

  • Phadke K, Nanda S (1983) Secretion of chondrocyte stimulating factor by macrophages as a result of activation with collagen and proteoglycans. Clin Exp Immunol 51:494–500

    Google Scholar 

  • Qi WN, Scully SP (1997) Extracellular collagen modulates the regulation of chondrocytes by transforming growth factor-β1. J Orthop Res 15:483–490

    Google Scholar 

  • Rosier RN, O'Keefe RJ (1998) Autocrine regulation of articular cartilage. Instr Course Lect 47:469–475

    Google Scholar 

  • Roughley PJ, Lee ER (1994) Cartilage proteoglycans: structure and potential function. Microsc Res Technol 28:385–397

    Google Scholar 

  • Roughley PJ, Nguyen Q, Mort SM (1991) Mechanisms of proteoglycan degradation in human articular cartilage. J Rheum 18:52–54

    Google Scholar 

  • Sandy PJ, Adams ME, Billingham MEJ, Plaas A, Muir H (1984) In vivo and in vitro stimulation of chondrocyte biosynthetic activity in early experimental osteoarthritis. Arthritis Rheum 27:388–397

    Google Scholar 

  • Sklatvala J (1986) Tumor necrosis factor-a stimulates resorption and inhibits synthesis of proteoglycan in cartilage. Nature 322:547–549

    Google Scholar 

  • Trippel SB (1995) Growth factor actions on articular cartilage. J Rheumatol 43:129–132

    Google Scholar 

  • von der Mark K, Gauss V, von der Mark H, Müller P (1977) Relationship between cell shape and type of collagen synthesised as chondrocytes lose their cartilage phenotype in culture. Nature 267:531–532

    Google Scholar 

  • Zaucke F, Dinser R, Maurer P, Paulsen M (2001) Cartilage oligomeric matrix protein (COMP) and collagen IX are sensitive markers for the differentiation state of articular primary chondrocytes. Biochem J 258:17–24

    Google Scholar 

Download references

Acknowledgements

We wish to thank Anja Raabe for her excellent technical assistance.

Author information

Authors and Affiliations

  1. Surgical Research of the Department of General Surgery and Thoracic Surgery of the University of Kiel, Michaelisstrasse 5, 24105, Kiel, Germany

    Steffen Oesser & Jürgen Seifert

Authors
  1. Steffen Oesser
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jürgen Seifert
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Steffen Oesser.

Additional information

We wish to thank the DGF Stoess AG for supporting this study.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Oesser, S., Seifert, J. Stimulation of type II collagen biosynthesis and secretion in bovine chondrocytes cultured with degraded collagen. Cell Tissue Res 311, 393–399 (2003). https://doi.org/10.1007/s00441-003-0702-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00441-003-0702-8

Keywords

Search

Navigation