Abstract
A new myxosporean species, Kudoa septempunctata n. sp. (Myxosporea: Multivalvulida), is described from the trunk muscles of an aquacultured olive flounder (Paralichthys olivaceus) imported from Korea. This species formed pseudocysts in the myofiber without inflammatory reactions, and the infection was not evident macroscopically. Spores of the new species were irregularly stellate in apical view, with the majority having seven unequal valves, each with a polar capsule of variable size (the remaining spores had six valves and polar capsules). The spores had dimensions of: width 11.8 (11.1–13.1); thickness 9.4 (8.9–10.0); length 8.5 (7.9–8.9); polar capsule length 4.6 (3.7–5.3); and polar capsule width 2.4 (2.2–2.8; mean with range in parentheses; n = 10; all measurements in micrometers). Scanning electron microscopy of the spores revealed unequal positioning of the seven valves without a definite center, rounded posterior ends of valves, and tiny projections at the apex of each valve. The small subunit ribosomal RNA gene (SSU rDNA) sequence of the new species was closely related to Kudoa spp. with five or more valves, particularly Kudoa thalassomi (97.6% identity) recorded from the trunk muscles of a moon wrasse (Thalassoma lunare) around the Australian continent. However, the latter species has six valves with a pointed edge and six polar capsules of a uniform size. The new species was also distinct from all presently known Kudoa spp. with seven valves and polar capsules, i.e. Kudoa yasunagai and Kudoa lethrini, regarding tissue tropism (trunk muscles versus brain), spore shape or external appendages, and SSU rDNA sequence.
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References
Adlard RD, Bryant MS, Whipps CM, Kent ML (2005) Multivalvulid myxozoans from Eastern Australia: three new species of Kudoa from scombrid and labrid fishes of the Great Barrier Reef, Queensland, Australia. J Parasitol 91:1138–1142
Anisimova M, Gascuel O (2006) Approximate likelihood-ratio test for branches: a fast, accurate, and powerful alternative. Syst Biol 55:539–552
Arai Y, Matsumoto K (1953) On a new sporozoa, Hexacapsula neothunni gen. et sp. nov., from the muscle of yellowfin tuna, Neothunnus macropterus. Bull Jpn Soc Sci Fish 18:293–298
Blaylock RB, Bullard SA, Whipps CM (2004) Kudoa hypoepicardialis n. sp. (Myxozoa: Kudoidae) and associated lesions from the heart of seven perciform fishes in the northern Gulf of Mexico. J Parasitol 90:584–593
Burger MAA, Cribb TH, Adlard RD (2007) Patterns of relatedness in the Kudoidae with descriptions of Kudoa chaetodoni n. sp. and K. lethrini n. sp. (Myxosporea: Multivalvulida). Parasitology 134:669–681
Burger MAA, Barnes AC, Adlard RD (2008) Wildlife as reservoirs for parasites infecting commercial species: host specificity and a redescription of Kudoa amamiensis from teleost fish in Australia. J Fish Dis 31:835–844
Dereeper A, Guignon V, Blanc G, Audic S, Buffet S, Chevenet F, Dufayard JF, Guindon S, Lefort V, Lescot M, Claverie JM, Gascuel O (2008) Phylogeny.fr: robust phylogenetic analysis for the non-specialist. Nucleic Acids Res 36:465–469
Diamant A, Ucko M, Paperna I, Colorni A, Lipshitz A (2005) Kudoa iwatai (Myxosporea: Multivalvulida) in wild and cultured fish in the red sea: redescription and molecular phylogeny. J Parasitol 91:1175–1189
Egusa S (1986) The order Multivalvulida Shulman, 1959 (Myxozoa: Myxosporea): a review. Fish Pathol 21:261–274 (in Japanese)
Egusa S, Shiomitsu T (1983) Two new species of the genus Kudoa (Myxosporea: Multivalvulida) from marine cultured fishes in Japan. Fish Pathol 18:163–171 (in Japanese with English summary)
Fiala I (2006) The phylogeny of Myxosporea (Myxozoa) based on small subunit ribosomal RNA gene analysis. Int J Parasitol 26:1521–1534
Grossel GW, Dykova I, Handlinger J, Munday BL (2003) Pentacapsula neurophila sp.n. (Multivalvulida) from the central nervous system of striped trumpeter, Latris lineata (Forster). J Fish Dis 26:315–320
Guindon S, Gascuel O (2003) A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 52:696–704
Hervio DML, Kent ML, Khattra J, Sakanari J, Yokoyama H, Devlin RH (1997) Taxonomy of Kudoa species (Myxosporea), using a small-subunit ribosomal DNA sequence. Can J Zool 75:2112–2119
Hsieh S, Chen C (1984) Septemcapsula yasunagai gen. et sp. nov., representative of a new family of the class Myxosporea. Acta Zootaxon Sin 9:225–227
Iversen ES, Van Meter NN (1967) A new myxosporidian (Sporozoa) infecting the Spanish mackerel. Bull Mar Sci 17:268–273
Kent ML, Andree KB, Bartholomew JL, El-Matbouli M, Desser SS, Devlin RH, Feist SW, Hedrick RP, Hoffmann RW, Khattra J, Hallett SL, Lester RJG, Longshaw M, Palenzuela O, Siddall ME, Xiao C (2001) Recent advances in our knowledge of the Myxozoa. J Eukaryot Microbiol 48:395–413
Kudo G, Barnett HJ, Nelson RW (1987) Factors affecting cooked texture quality of Pacific whiting, Merluccius productus, fillets with particular emphasis on the effects of infection by the myxosporeans Kudoa paniformis and K. thyrsitis. Fish Bull 85:745–756
Lom J, Arthur JR (1989) A guideline for the preparation of species descriptions in Myxosporea. J Fish Dis 12:151–156
Lom J, Dyková I (2006) Myxozoan genera: definition and notes on taxonomy, life-cycle terminology and pathogenic species. Folia Parasitol 53:1–36
Maeno Y, Nagasawa K, Sorimachi M (1993) Kudoa intestinalis n. sp. (Myxosporea: Multivalvulida) from the intestinal musculature of the striped mullet, Mugil cephalus, from Japan. J Parasitol 79:190–192
Martínez de Velasco G, Rodero M, Cuéllar C, Chivato T, Mateos JM, Laguna R (2008) Skin prick test of Kudoa sp. antigens in patients with gastrointestinal and/or allergic symptoms related to fish ingestion. Parasitol Res 103:713–715
Moran JDW, Whitaker DJ, Kent ML (1999) A review of the myxosporean genus Kudoa Meglitsch, 1947, and its impact on the international aquaculture industry and commercial fisheries. Aquaculture 172:163–196
Nakajima K, Egusa S (1978) Kudoa pericardialis n.sp. (Myxosporidea: Chloromyxidae) from cultured yellowtail. Seriola quinqueradiata Temminck et Schlegel. Bull Jpn Soc Sci Fish 44:117–120
Sitjà-Bobadilla A (2009) Can Myxosporean parasites compromise fish and amphibian reproduction? Proc R Soc B 276:2861–2870
Smythe AB, Sanderson MJ, Nadler SA (2006) Nematode small subunit phylogeny correlates with alignment parameters. Syst Biol 55:972–992
Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
Whipps CM, Kent M (2006) Phylogeography of the cosmopolitan marine parasite Kudoa thyrsites (Myxozoa: Myxosporea). J Eukaryot Microbiol 53:364–373
Whipps CM, Adlard RD, Bryant MS, Kent ML (2003) Two unusual myxozoans, Kudoa quadricornis n. sp. (Multivalvulida) from the muscle of goldspotted trevally (Carangoides fulvoguttatus) and Kudoa permulticapsula n. sp. (Multivalvulida) from the muscle of Spanish mackerel (Scomberomorus commerson) from the great barrier reef, Australia. J Parasitol 89:168–173
Whipps CM, Grossel G, Adlard RD, Yokoyama H, Bryant MS, Munday BL, Kent ML (2004) Phylogeny of the Multivalvulidae (Myxozoa: Myxosporea) based on comparative ribosomal DNA sequence analysis. J Parasitol 90:618–622
Yokoyama H (2003) A review: gaps in our knowledge on myxozoan parasites of fishes. Fish Pathol 38:125–136
Yokoyama H, Whipps CM, Kent ML, Mizuno K, Kawakami H (2004) Kudoa thyrsites from Japanese flounder and Kudoa lateolabracis n. sp. from Chinese sea bass: causative myxozoans of post-mortem myoliquefaction. Fish Pathol 39:79–85
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Matsukane, Y., Sato, H., Tanaka, S. et al. Kudoa septempunctata n. sp. (Myxosporea: Multivalvulida) from an aquacultured olive flounder (Paralichthys olivaceus) imported from Korea. Parasitol Res 107, 865–872 (2010). https://doi.org/10.1007/s00436-010-1941-8
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DOI: https://doi.org/10.1007/s00436-010-1941-8