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Molecular cloning and characterization of a phosphoglycerate mutase gene from Clonorchis sinensis

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Abstract

Phosphoglycerate mutase (PGM) is a widely distributed glycolytic enzyme. Two known distinct classes of PGM enzymes were identified, a cofactor-dependent one (dPGM) and a cofactor-independent one (iPGM). A complementary DNA (cDNA) encoding a PGM was cloned from a Clonorchis sinensis cDNA library by large-scale sequencing. This new cDNA contains 955 bp with a putative open reading frame of 256 amino acids, which has a high homology with dPGMs from a number of species. The putative peptide was produced in E. coli and was purified to electrophoretic homogeneity. Enzymatic assays showed that the product of this gene could catalyze the conversion of 3-phosphoglycerate to 2-phosphoglycerate when the cofactor was present and the enzyme activities could be inhibited by vanadate.

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Acknowledgment

This research was supported by grants from the Natural Science Foundation of Guangdong Province, China (No.2002B31005) and the Key Program of Science and Technology Department of Guangdong Province, China (No.200223-E4022). The experiments comply with the current laws of the country in which the experiments were performed.

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Authors and Affiliations

  1. College of Life Science, Shandong University of Technology, Zibo, 255049, People’s Republic of China

    Linxia Song & Zhenbiao Xu

  2. Department of Parasitology, Medical School, Sun Yat-Sen University, Guangzhou, 510089, People’s Republic of China

    Xinbing Yu

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  1. Linxia Song
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  2. Zhenbiao Xu
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  3. Xinbing Yu
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Correspondence to Xinbing Yu.

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Song, L., Xu, Z. & Yu, X. Molecular cloning and characterization of a phosphoglycerate mutase gene from Clonorchis sinensis . Parasitol Res 101, 709–714 (2007). https://doi.org/10.1007/s00436-007-0540-9

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  • DOI: https://doi.org/10.1007/s00436-007-0540-9

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