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The Influence of Plant Growth Regulators and Light Quality on Somatic Embryogenesis in China Rose (Rosa chinensis Jacq.)

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Abstract

We investigated the effect of red light and plant growth regulators on somatic embryogenesis in China Rose (Rosa chinensis Jacq.). Embryogenic calli that had been induced by combinations of 2,4-dichlorophenoxyacetic acid and thidiazuron in darkness were exposed to dark, red, and white light treatments. Cultures subjected to red light treatment generated the greatest number of embryos, with one (SE1 embryos) or two (SE2 embryos) expanded cotyledons. The largest numbers of shoot-like embryos without cotyledons (SE0 embryos) were produced in cultures subjected to dark treatment. The effects of different concentrations of abscisic acid (ABA) on the proliferation and germination of different types of somatic embryos were also evaluated. A concentration of 9.45 μM was found to be the most effective in promoting the proliferation and germination of SE2 embryos. The higher the concentration of ABA (from 0 to 18.90 μM), the higher the percentage of abnormal polycotyledonary embryos produced. The highest percentage of regenerated plants was obtained from SE2 embryos.

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References

  • Aldwinckle HS, Malnoy M (2009) Plant regeneration and transformation in the Rosaceae. Transgenic Plant J 3(Special Issue 1):1–39

    Google Scholar 

  • Ammirato PV (1974) The effects of abscisic acid on the development of somatic embryos from cells of caraway (Carum caryi L.). Bot Gaz 135:328–337

    Article  CAS  Google Scholar 

  • Burger DW, Liu L, Zary KW, Lee CI (1990) Organogenesis and plant regeneration from immature embryos of Rosa hybrida L. Plant Cell Tiss Org Cult 21:147–152

    Article  Google Scholar 

  • Castillon J, Kamo K (2002) Maturation and conversion of somatic embryos of three genetically diverse rose cultivars. HortScience 37:973–977

    Google Scholar 

  • Chen JR, Liu R, Wang HF (2006) Plant regeneration of transgenic China Rose (Rosa chinesis Jacq.) from organogenic callus. For Stud China 8:92–97

    Article  Google Scholar 

  • Chen JR, Lü JJ, Liu R, Xiong XY, Guo LB, Wang HF (2010) DREB1C from Medicago truncatula enhances freezing tolerance in transgenic M. truncatula and China Rose (Rosa chinensis Jacq.). Plant Growth Regul 60:199–211

    Article  CAS  Google Scholar 

  • D’Onofrio C, Morini S, Bellocchi G (1998) Effect of light quality on somatic embryogenesis of quince leaves. Plant Cell Tiss Org Cult 53:91–98

    Article  Google Scholar 

  • De Wit JC, Esendam HF, Honkanen JJ, Tuominen U (1990) Somatic embryogenesis and regeneration of flowering plants in rose. Plant Cell Rep 9:456–458

    Article  PubMed  Google Scholar 

  • Dubois LAM, de Vries DP (1995) Preliminary report on the direct regeneration of adventitious buds on leaf explants of in vitro grown glass house rose cultivars. Gartenbauwissenschaft 60:249–253

    CAS  Google Scholar 

  • Firoozabady E, Moy Y, Courtney-Gutterson N, Robinson K (1994) Regeneration of transgenic rose (Rosa hybrida) plants from embryogenic tissue. Biotechnology 12:609–613

    Article  CAS  Google Scholar 

  • Hill GP (1967) Morphogenesis of shoot primordia in cultured stem tissue of a garden rose. Nature 216:596–597

    Article  Google Scholar 

  • Hsia CN, Korban SS (1996) Organogenesis and somatic embryogenesis in callus cultures of Rosa hybrida and Rosa chinensis Minima. Plant Cell Tiss Org Cult 44:1–6

    Article  CAS  Google Scholar 

  • Huang SW (2000) Rose Breeding. In: Cheng JS (ed) The Genetics and Breeding of Garden Plants. China Forestry Publishing House, Beijing, pp 385–402

    Google Scholar 

  • Ibrahim R, Debergh PC (2001) Factors controlling high efficiency adventitious bud formation and plant regeneration from in vitro leaf explant of roses (Rosa hybrid L.). Sci Hortic 88:41–57

    Article  Google Scholar 

  • Kim SW, Oh SC, Liu JR (2003) Control of direct and indirect somatic embryogenesis by exogenous growth regulators in immature zygotic embryo cultures of rose. Plant Cell Tiss Org Cult 74:61–66

    Article  CAS  Google Scholar 

  • Kim CK, Oh JY, Chung JD, Burrell AM, Byrne DH (2004) Somatic embryogenesis and plant regeneration from in vitro-grown leaf explants of rose. HortScience 39(6):1378–1380

    CAS  Google Scholar 

  • Kim SW, Oh MJ, Liu JR (2009) Somatic embryogenesis and plant regeneration in zygotic embryo explant cultures of rugosa rose. Plant Biotechnol Rep 3:199–203

    Article  Google Scholar 

  • Kintzios S, Manos C, Makri O (1999) Somatic embryogenesis from mature leaves of rose (Rosa sp.). Plant Cell Rep 18:467–472

    Article  CAS  Google Scholar 

  • Kunitake H, Imamizo H, Mii H (1993) Somatic embryogenesis and plant regeneration from immature seed-derived calli of rugosa rose (Rosa rugosa Thurb.). Plant Sci 90:187–194

    Article  CAS  Google Scholar 

  • Li XQ, Krasnyanski SF, Korban SS (2002) Somatic embryogenesis, secondary somatic embryogenesis, and shoot organogenesis in Rosa. J Plant Physiol 159:313–319

    Article  CAS  Google Scholar 

  • Marchant R, Davey MR, Lucas JA, Power JB (1996) Somatic embryogenesis and plant regeneration in Floribunda rose (Rosa hybrida L.) cvs. Trumpeter and glad tidings. Plant Sci 120:95–105

    Article  Google Scholar 

  • Merkle SA, Montello PM, Xia X, Upchurch BL, Smith DR (2005) Light quality treatments enhance somatic seedling production in three southern pine species. Tree Physiol 26:187–194

    Article  Google Scholar 

  • Michler CH, Lineberger RD (1987) Effects of light on somatic embryo development and abscisic levels in carrot suspension cultures. Plant Cell Tiss Org Cult 11:189–207

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol 15:473–497

    Article  CAS  Google Scholar 

  • Noriega C, Söndahl MR (1991) Somatic embryogenesis in hybrid tea roses. Biotechnology 9:991–993

    Article  Google Scholar 

  • Rai MK, Shekhawat NS, Harish, Gupta AK, Phulwaria M, Ram K, Jaiswal U (2011) The role of abscisic acid in plant tissue culture—a review on the recent progress. Plant Cell Tissue Organ Cult 106:179–190

    Article  CAS  Google Scholar 

  • Rosu A, Skirvin RM, Bein A, Norton MA, Kushad M, Otterbacher AG (1995) The development of putative adventitious shoots from a chimeral thornless rose (Rosa multiflora Thurb. ex J Murr.) in vitro. J Hort Sci 70:901–907

    Google Scholar 

  • Rout GR, Debata BK, Das P (1991) Somatic embryogenesis in callus cultures of Rosa hybrida L. cv Landora. Plant Cell Tiss Org Cult 27:65–69

    Article  CAS  Google Scholar 

  • Rout GR, Samantaray S, Das P (1992) Chloropromazine induced in vitro bud break in Rosa hybrida cv Landora. Orissa J Horticult 20:8–16

    Google Scholar 

  • Sarasan V, Roberts AV, Rout GR (2001) Methyl laurate and 6-benzyl-adenine promote the germination of somatic embryos of a hybrid rose. Plant Cell Rep 20:183–186

    Article  CAS  Google Scholar 

  • Schenk RU, Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 50:199–204

    Article  CAS  Google Scholar 

  • Umbeck PF (1981) Does ABA play a role in seed germination? Israel J Bot 29:168–180

    Google Scholar 

  • van der Salm TPM, van der Toorn CJG, Hanischten CH, Dons HJM (1996) Somatic embryogenesis and shoot regeneration from excised adventitious roots of the root stock Rosa hybrida cv Money Way. Plant Cell Rep 15:522–526

    Article  PubMed  Google Scholar 

  • Vergne P, Maene M, Gabant G, Chauvet A, Debener T, Bendahmane M (2010) Somatic embryogenesis and transformation of the diploid Rosa chinensis cv Old Blush. Plant Cell Tiss Organ Cult 100:73–81

    Article  Google Scholar 

  • Visessuwan R, Kawai T, Mii M (1997) Plant regeneration systems from leaf segment culture through embryogenic callus formation of Rosa hybrida and R. canina. Breed Sci 47:217–222

    CAS  Google Scholar 

  • Yang XY, Zhang XL (2010) Regulation of somatic embryogenesis in higher plants. Crit Rev Plant Sci 29:36–57

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant Nos. 31071826 and 31272208), the Special Research Fund for Doctor’s Degree Dissertation in Chinese Universities (Grant No. 20104320120008), the China Postdoctoral Science Foundation (Grant Nos. 20100471215 and 201104473), and Hunan graduates’ innovation fund (CX2012B298). English-language editing was supplied by International Science Editing and Edanz Editing.

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Authors and Affiliations

  1. College of Horticulture and Gardening, Hunan Agricultural University, Changsha, 410128, People’s Republic of China

    Ji-Ren Chen, Lian Wu, Bo-Wen Hu, Xing Yi, Zi-Niu Deng & Xing-Yao Xiong

  2. Hunan Provincial Key Laboratory for Germplasm Innovation and Utilization of Crops, Changsha, 410128, People’s Republic of China

    Zi-Niu Deng & Xing-Yao Xiong

  3. The Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, People’s Republic of China

    Xing-Yao Xiong

  4. Hunan Biological and Electromechanical Polytechnic, Changsha, 410127, People’s Republic of China

    Rong Liu

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  1. Ji-Ren Chen
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  2. Lian Wu
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  3. Bo-Wen Hu
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  4. Xing Yi
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  5. Rong Liu
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  6. Zi-Niu Deng
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  7. Xing-Yao Xiong
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Correspondence to Xing-Yao Xiong.

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Ji-Ren Chen, Lian Wu and Bo-Wen Hu have contributed equally to this study.

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Chen, JR., Wu, L., Hu, BW. et al. The Influence of Plant Growth Regulators and Light Quality on Somatic Embryogenesis in China Rose (Rosa chinensis Jacq.). J Plant Growth Regul 33, 295–304 (2014). https://doi.org/10.1007/s00344-013-9371-3

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  • DOI: https://doi.org/10.1007/s00344-013-9371-3

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