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Hymenobacter baengnokdamensis sp. nov., Isolated from the Soil of a Crater Lake in Korea

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Abstract

An aerobic, Gram-stain-negative, non-motile, non-spore-forming, rod-shaped and pink-colored bacterial strain, designated BRD72T, was isolated from a crater lake (Baengnokdam) at the top of Mt. Hallasan in the Republic of Korea. Cells were catalase-positive and oxidase-negative. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that the isolate was a member of the genus Hymenobacter and most closely related to Hymenobacter marinus KJ035T (96.2% similarity). The isolate was found to produce carotenoid pigment, but not flexirubin-type pigment. The predominant fatty acids of strain BRD72T were summed feature 3 (C16:1 ω7c and/or C16:1 ω6c, 21.6%), iso-C15:0 (17.9%), anteiso-C15:0 (13.3%) and summed feature 4 (iso-C17:1 I and/or anteiso-C17:1 B, 11.3%). The major polar lipids were phosphatidylethanolamine, an unidentified amino lipid, and two unidentified aminophospholipids. The main respiratory quinone was menaquinone-7 (MK-7), and the main polyamine was homospermidine. The DNA G+C content was 59.8 mol%. Based on the phylogenetic, physiological, and chemotaxonomic characteristics, strain BRD72T represents a novel species, for which the name Hymenobacter baengnokdamensis sp. nov. is proposed. The type strain is BRD72T (= KCTC 72649T = JCM 33837T).

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Acknowledgements

This research was supported by the Nuclear R&D program of the Ministry of Science and ICT (MSIT), Republic of Korea.

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Authors and Affiliations

  1. Radiation Research Division, Korea Atomic Energy Research Institute, Jeongeup, 56212, Republic of Korea

    Ji Hee Lee, Min-Kyu Kim, Jong-Hyun Jung, Ho Seong Seo, Jing Zhang & Sangyong Lim

  2. Department of Radiation Science and Technology, University of Science and Technology, Daejeon, 34113, Republic of Korea

    Jong-Hyun Jung, Ho Seong Seo & Sangyong Lim

  3. Department of Biotechnology and Bioengineering, Interdisciplinary Program for Bioenergy and Biomaterials, Chonnam National University, Gwangju, 61186, Republic of Korea

    Jing Zhang

  4. Biological Resource Center, Korea Research Institute of Bioscience & Biotechnology, Jeongeup, 56212, Republic of Korea

    Han Na Choe

  5. Department of Biology, College of Life Science and Natural Resources, Sunchon National University, Suncheon, 57922, Republic of Korea

    Seon Choi & Chi Nam Seong

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  1. Ji Hee Lee
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Contributions

JHL performed the experiments and wrote the manuscript. JHJ analyzed the genome sequence. HSS and JZ isolated and identified the strain. HNC and SC tested the chemotaxonomic characterizations. MKK and CNS guided the experiments and data analysis. SL conceived the study and was in charge of overall direction and planning. All authors approved the manuscript and its submission.

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Correspondence to Sangyong Lim.

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Accession number

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain BRD72T is MK752551. The GenBank/EMBL/DDBJ accession number for the whole genome sequence of strain BRD72T is CP044285.

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Lee, J.H., Kim, MK., Jung, JH. et al. Hymenobacter baengnokdamensis sp. nov., Isolated from the Soil of a Crater Lake in Korea. Curr Microbiol 77, 4167–4173 (2020). https://doi.org/10.1007/s00284-020-02225-7

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