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Halobellus litoreus sp. nov., a Halophilic Archaeon Isolated from a Chinese Marine Solar Saltern

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Abstract

Halophilic archaeal strain GX31T was isolated from a marine solar saltern of China. The cells of the strain were rod-shaped and lysed in distilled water, stain Gram-negative and formed red-pigmented colonies. It was neutrophilic, and required at least 0.9 M NaCl and 0–1.0 M MgCl2 for growth under the optimum growth temperature of 37 °C. The major polar lipids of the strain were phosphatidylglycerol (PG), PG phosphate methyl ester, PG sulphate, and two major glycolipids chromatographically identical to sulphated mannosyl glucosyl diether (S-DGD-1) and mannosyl glucosyl diether (DGD-1), respectively. Trace amounts of two unidentified lipids were also detected. On the basis of 16S rRNA gene sequence analysis, strain GX31T was closely related to the members of Halobellus of the family Halobacteriaceae with similarities of 94.1–98.7 %. Strain GX31T showed 89.8–95.4 % of the rpoB′ gene similarity to the members of Halobellus. The DNA G+C content of strain GX31T was 66.8 mol%. Strain GX31T showed low DNA–DNA relatedness with two most related members of the genus Halobellus. The phenotypic, chemotaxonomic and phylogenetic properties suggest that strain GX31T represent a novel species of the genus Halobellus, for which the name Halobellus litoreus sp. nov. is proposed. The type strain is GX31T (=CGMCC 1.10387T = JCM 17118T).

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 31370054), the grant from China Ocean Mineral Resources R&D Association (COMRA) Special Foundation (DY125-15-R-03), the Qinglan Project of Jiangsu Province and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Authors and Affiliations

  1. School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People’s Republic of China

    Mei-Lin Zhao, Xing-Xing Qiu, Wen-Jiao Zhang, Dong Han & Heng-Lin Cui

  2. Department of Cell Biology, Nanjing Medical University, 140 Hanzhong Road, Gulou District, Nanjing, 210029, People’s Republic of China

    Zheng-Rong Li

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  1. Mei-Lin Zhao
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  2. Xing-Xing Qiu
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  4. Dong Han
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  5. Heng-Lin Cui
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Correspondence to Heng-Lin Cui or Zheng-Rong Li.

Additional information

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene and the rpoB′ gene sequences of strain GX31T are GU951426 and KF241979, respectively. Phase-contrast micrograph and thin-layer chromatogram of strain GX31T, maximum-parsimony and maximum-likelihood phylogenetic trees based on 16S rRNA gene and the rpoB′ gene sequences of strain GX31T and some related haloarchaea are available as supplementary materials.

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Zhao, ML., Qiu, XX., Zhang, WJ. et al. Halobellus litoreus sp. nov., a Halophilic Archaeon Isolated from a Chinese Marine Solar Saltern. Curr Microbiol 68, 156–160 (2014). https://doi.org/10.1007/s00284-013-0457-8

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  • DOI: https://doi.org/10.1007/s00284-013-0457-8

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