Skip to main content
Log in

Development of Genus/Species-Specific PCR Analysis for Identification of Carnobacterium Strains

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

Heterofermentative lactic acid bacteria belonging to the genus Carnobacterium are currently divided into seven different species, C. piscicola, C. mobile, C. gallinarum, C. inhibens, C. divergens, C. funditum, and C. alterfunditum. 16S rDNA-targeted PCR assay was carried out for the identification of the genus Carnobacterium. In addition, type strains of all Carnobacterium species were analyzed by 16S–23S rDNA intergenic spacer analysis in comparison with type strains of phylogenetically related lactic acid bacteria. These methods enabled the identification and the discrimination among Carnobacterium species and the other phylogenetically related lactic acid bacteria. Likewise, analogous results were obtained by restriction analysis of amplified 16S rDNA performed with HaeIII and HinfI as restriction enzymes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Author information

Authors and Affiliations

Authors

Additional information

Received: 25 July 2001 / Accepted: 19 October 2001

Rights and permissions

Reprints and permissions

About this article

Cite this article

Scarpellini, M., Mora, D., Colombo, S. et al. Development of Genus/Species-Specific PCR Analysis for Identification of Carnobacterium Strains. Curr Microbiol 45, 24–29 (2002). https://doi.org/10.1007/s00284-001-0043-3

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00284-001-0043-3

Keywords

Navigation