Abstract
A novel sulfate-reducing bacterium, strain ASN36T, was isolated from sediment of a brackish lake in Japan. Cells of strain ASN36T were not motile and rod-shaped, with length of 2.0–4.9 μm and width of 0.6–0.9 μm. Growth was observed at 5–35 °C with an optimum growth temperature of 25–30 °C. The pH range for growth was 6.6–8.8 with an optimum pH of 7.3. Major fatty acids were C16:1 ω7c and C16:0. Under sulfate-reducing conditions, strain ASN36T utilized lactate, malate, pyruvate, butyrate, ethanol, butanol, glycerol, yeast extract and H2/CO2 as growth substrate. Fermentative growth occurred on malate and pyruvate. The novel isolate used sulfate, sulfite and thiosulfate as electron acceptors. The genome of strain ASN36T is composed of a chromosome with length of 6.3 Mbp and G + C content of 55.1 mol%. Analyses of the 16S rRNA gene indicated that strain ASN36T is related to Desulfoluna species. Overall genome relatedness indices indicated that strain ASN36T does not belong to any existing species. In contrast to the closest relatives, strain ASN36T lacks genes for reductive dehalogenase required for organohalide respiration and does not use halogenated aromatics as electron acceptors. On the basis of its genomic and phenotypic properties, strain ASN36T (= DSM 111985 T = JCM 39257 T) is proposed as the type strain of a new species, Desulfoluna limicola sp. nov.
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Acknowledgements
This research was supported by the Institute of Low Temperature Science, Hokkaido University. We are grateful to the crew of the Umiaisa, Akkeshi Marine Station, Hokkaido University for their technical expertise. We wish to thank T. Nakagawa (Nihon University) for his support with field work. We thank A. Shinohara (Hokkaido University) for technical assistance in strain maintenance.
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MF and AT collected the sediment sample. AT isolated the strain. AT and MW performed experiments for phenotypic characterization, and conducted genome analysis. MW and HK wrote the main manuscript text and prepared figure 1. All authors reviewed the manuscript.
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Watanabe, M., Takahashi, A., Kojima, H. et al. Desulfoluna limicola sp. nov., a sulfate-reducing bacterium isolated from sediment of a brackish lake. Arch Microbiol 204, 640 (2022). https://doi.org/10.1007/s00203-022-03259-0
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DOI: https://doi.org/10.1007/s00203-022-03259-0