Abstract
In this study, strain CAU 1523T, a novel Gram-positive-positive bacterium isolated from marine sediment collected from the coast of Busan, Republic of Korea, was characterized using a polyphasic taxonomic approach. This strain showed growth at a temperature range of 20–37 °C (optimum, 30 °C), a pH range of 6.5–9.5 (optimum, 7.5), and in the presence of 0–3% (w/v) NaCl (optimum, 1%). Phylogenetic analysis based on 16S rRNA gene sequencing and 92 concatenated core genes indicated that CAU 1523T belonged to the genus Paenibacillus, sharing the highest sequence similarity with P. assamensis JCM 13186T (98.0%). CAU 1523T was differentiated from other Paenibacillus species by average nucleotide identity, average amino acid identity, and digital DNA–DNA hybridization values, using cut-off values of 95–96%, 90%, and 70%, respectively, for closely related strains. The genome of CAU 1523T possessed various biosynthetic gene clusters, one of which encoded a putative siderophore-interacting protein. Siderophore production by the isolate was confirmed using the qualitative chrome azurol sulfonate (CAS) agar assay. Based on its phylogenetic and physiological characteristics, strain CAU 1523T represents a novel, siderophore-producing species within the genus Paenibacillus, for which the name Paenibacillus arenosi sp. nov. is proposed, with the type strain CAU 1523T (= KCTC 43108T = MCCC 1K04063T).
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Data availability
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain CAU 1523T is MN097941 and the whole genome shotgun project accession number is JACYTN000000000.
Code availability
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Abbreviations
- AAI:
-
Amino acid identity
- ANI:
-
Average nucleotide identity
- BGCs:
-
Biosynthetic gene clusters
- BHI:
-
Brain–heart infusion agar
- CAS:
-
Chrome azurol sulfonate
- dDDH:
-
DNA–DNA hybridization
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This work was supported by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR201902203) and the Chung-Ang University Research Grant in 2020.
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Conceived and designed the experiments: WK and J-HY. Conducted the experiments: JB, VW, and J-SL. Analyzed the data: J-HK, J-HY, and AS. Contributed reagents, materials, and analysis tools: WK. Wrote the paper: JB, VW, and WK.
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Communicated by Erko Stackebrandt.
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Baek, J., Weerawongwiwat, V., Kim, JH. et al. Paenibacillus arenosi sp. nov., a siderophore-producing bacterium isolated from coastal sediment. Arch Microbiol 204, 113 (2022). https://doi.org/10.1007/s00203-021-02735-3
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DOI: https://doi.org/10.1007/s00203-021-02735-3