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Sandaracinobacteroides hominis gen. nov., sp. nov., isolated from human skin

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Abstract

Strain SZY PN-1 T, representing a novel Gram-negative, aerobic, non-motile, rod-shaped and yellow-pigmented bacterium, was isolated from a skin sample of a healthy Chinese male. Growth occurred at pH 6.0–8.0 (optimum, pH 7.0) and 10–37 ℃ (optimum, 30 ℃) with 0–1.0% (w/v) NaCl in R2A agar. Comparative analysis of the 16S rRNA gene sequences revealed that strain SZY PN-1 T shared high similarities with two invalid-published species, “Sandaracinobacter sibiricus” RB16-17 (97.1%) and “Sandaracinobacter neustonicus” JCM 30734 (96.6%), respectively. Phylogenetic analysis of 16S rRNA gene sequences together with protein-concatemer tree showed that SZY PN-1 T formed a separate branch within the family Sphingosinicellaceae. The DNA G + C content of the strain SZY PN-1 T was 65.0% (genome). The polar lipid profile included phosphatidylethanolamine, phosphatidylglycerol, two sphingoglycolipids, diphosphatidylglycerol, five unidentified glycolipids, and seven unidentified lipids. The predominant fatty acids (> 10.0%) were identified as C18:1 ω7c and/or C18:1 ω6c, C17:1 ω6c, C16:1 ω7c and/or C16:1 ω6c. The major respiratory quinone was Q-10. Based on the phenotypic and genotypic features, a novel genus and species, Sandaracinobacteroides hominis gen. nov., sp. nov. is proposed, with type strain SZY PN-1 T (= KCTC 82150 T = NBRC 114675 T).

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Acknowledgements

This research was supported by Guangdong Provincial Key Laboratory of Chinese Medicine for Prevention and Treatment of Refractory Chronic Diseases (2018B030322012) and National Natural Science Foundation of China (31972856).

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  1. Ping-Hua Qu and Hai-Min Luo have contributed equally to this work.

Authors and Affiliations

  1. Department of Clinical Laboratory, The Second Clinical College of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou, 510006, People’s Republic of China

    Ping-Hua Qu, Hai-Min Luo, Jun-Hui Feng, Song Li & Cha Chen

  2. State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

    Lei Dong, Yu-Zhen Ming & Wen-Jun Li

  3. Department of Dermatology, Guangdong Provincial Key Laboratory of Chinese Medicine for Prevention and Treatment of Refractory Chronic Diseases, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou, 510006, People’s Republic of China

    Ying Lin

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Contributions

YL and CC designed the research and project outline. PHQ, HML, JHF, SL, LD and YZM performed isolation, deposition, and identification. PHQ, HML and JHF analyzed the data. PHQ, HML and WJL drafted the manuscript. All authors read and approved the final manuscript.

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Correspondence to Cha Chen or Ying Lin.

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All the authors have declared that there are no conflicts of interest.

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All experiments involving human subjects were carried out according to the institutional review board protocols approved by the Medical Ethics Committee of Guangdong Provincial Hospital of Chinese Medicine in China (BE2019-165). Informed consent was obtained from all subjects.

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Communicated by Erko Stackebrandt.

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Qu, PH., Luo, HM., Feng, JH. et al. Sandaracinobacteroides hominis gen. nov., sp. nov., isolated from human skin. Arch Microbiol 203, 5067–5074 (2021). https://doi.org/10.1007/s00203-021-02454-9

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