Abstract
In this study, we report a novel Gram-positive bacterium, designated as strain CS13T, isolated from deep-sea sediment collected in the cold seep area of the South China Sea. Growth of strain CS13T occurred at 16–37 °C (optimum 25–28 °C), pH 7.0–9.0 (optimum, 7.0), and 0–8% (w/v) NaCl (optimum, 2–3%). Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain CS13T belonged to the genus Bacillus. The closest phylogenetic neighbors of strain CS13T are Bacillus carboniphilus JCM 9731T (96.0%), Bacillus pakistanensis NCCP-168T (95.7%) and Bacillus acidicola 105-2T (95.6%). The genomic DNA G + C content of strain CS13T is 43.7 mol%. The principal respiratory quinone was menaquinone 7 (MK—7). The polar lipids of CS13T contained diphosphatidylglycerol, phosphatidylglycerol, phospholipid, and glycolipid. The major fatty acids of CS13T contained anteiso-C15:0, anteiso-C17:0, C16:0 and C18:0. Strain CS13T harboured meso-diaminopimelic acid as the diagnostic diamino acid. Phylogenetic, physiological, biochemical, and morphological analyses suggested that strain CS13T represents a novel species of genus Bacillus, and the name Bacillus fonticola sp. nov. is proposed for the type species CS13T (= CCTCC AB 2019194T = JCM 33663T).
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Data availability
The GenBank accession number of the 16S rRNA gene sequence of strain CS13T is MT359011. The GenBank accession number of complete genome sequence of strain CS13T is CP052135.
Abbreviations
- ANI:
-
Average nucleotide identity
- DDH:
-
DNA–DNA hybridization
- DPG:
-
Diphosphatidylglycerol
- DSMZ:
-
Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
- FAMEs:
-
Fatty acid methyl esters
- GL:
-
Glycolipid
- MA:
-
Marine agar
- ME:
-
Minimum evolution
- MK-7:
-
Menaquinone 7
- ML:
-
Maximum likelihood
- NJ:
-
Neighbor joining
- PL:
-
Phospholipid
- PG:
-
Phosphatidylglycerol
- pNPG:
-
P-nitrophenyl-β-d-galactopyranoside
- rRNA:
-
Ribosome RNA
- tRNA:
-
Transfer RNA
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Acknowledgements
The authors acknowledge the support of the Research Vessel KEXUE of the National Major Science and Technology Infrastructure from the Chinese Academy of Sciences. This work was funded by the grants of the National Key R&D Program of China (2018YFC0310801), Qingdao National Laboratory for Marine Science and Technology (QNLM2016ORP0309), The Senior User Project of RV KEXUE (KEXUE2018G20), and the Taishan Scholar Program of Shandong Province.
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QLS designed the work. YYS and HZZ conducted the laboratory experiments. YYS analyzed the data and drafted the manuscript. QLS supervised the study and revised the manuscript. All authors read and approved the final version of the manuscript.
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Communicated by Erko Stackebrandt.
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Sun, Yy., Zhou, Hz. & Sun, Ql. Bacillus fonticola sp. nov., isolated from deep sea cold seep sediment. Arch Microbiol 203, 4127–4132 (2021). https://doi.org/10.1007/s00203-021-02401-8
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DOI: https://doi.org/10.1007/s00203-021-02401-8