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Tenacibaculum pelagium sp. nov., isolated from marine sediment

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Abstract

A novel bright yellow pigmented, Gram-stain-negative, gliding, aerobic and rod-shaped marine bacterium, designated strain S7007T, was isolated from a marine sediment sample taken from Jingzi Wharf, Weihai, China. The bacterium was able to grow at 4–33 °C (optimum 28 °C), at pH 6.5–9.0 (optimum 7.0) and with 2.0–4.0% (w/v) NaCl (optimum 3.0%). According to the phylogenetic analysis based on the 16S rRNA gene sequences, strain S7007T was associated with the genus Tenacibaculum and showed highest similarity to Tenacibaculum adriaticum JCM 14633T (98.0%). The average nucleotide identity (ANI) scores of strain S7007T with T. adriaticum JCM 14633T and T. maritimum NBRC 110778T were 78.3% and 77.1%, respectively and the Genome-to-Genome Distance Calculator (dDDH) scores were 20.5% and 19.9%, respectively. The sole isoprenoid quinone was MK-6 and the major cellular fatty acids (> 10.0%) were iso-C15:0, iso-C15:0 3-OH, iso-C15: 1 G and summed feature 3 (comprising C16:1 ω7c and/or C16:1 ω6c). The major polar lipids of strain S7007T were phosphatidylethanolamine, phosphatidyldimethylethanolamine, one unidentified lipid and two unidentified aminolipids. The genomic DNA G + C content was 30.9 mol %. The combined phenotypic data and phylogenetic inference that strain S7007T should be classified as a novel species in the genus Tenacibaculum, for which the name Tenacibaculum pelagium sp. nov. is proposed. The type strain is S7007T (= MCCC 1H00428T = KCTC 72941T).

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Data availability

The GenBank accession number for the 16S rRNA gene sequence and whole-genome shotgun project of strain S7007T are MT822280 and JACGLS000000000, respectively.

Abbreviations

JCM:

Japan Collection of Microorganisms

MCCC:

Marine Culture Collection of China

NBRC:

Biological Resource Center

KCTC:

The Korean Collection for Type Cultures

ANI:

Average nucleotide identity

GGDC:

Genome-to-Genome Distance Calculator

dDDH:

Digital DNA–DNA hybridization

MEGA:

Molecular Evolutionary Genetics Analysis

MIDI:

Microbial Identification System

HPLC:

High Performance Liquid Chromatography

TLC:

Thin layer chromatography

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Acknowledgements

This work of scanning electron microscope was supported by the Physical-Chemical Materials Analytical & Testing Center of Shandong University at Weihai.

Funding

This work was supported by the National Natural Science Foundation of China (32070002, 31770002) and National Science and Technology Fundamental Resources Investigation Program of China (2019FY100700).

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Authors and Affiliations

  1. Marine College, Shandong University, Weihai, 264209, Shandong, People’s Republic of China

    Dan-Dan Shang, He-Yuan Lun, Ke-Lei Zhu, Guan-Jun Chen & Zong-Jun Du

  2. State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, Shandong, People’s Republic of China

    Guan-Jun Chen & Zong-Jun Du

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  1. Dan-Dan Shang
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  2. He-Yuan Lun
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  3. Ke-Lei Zhu
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Contributions

Strain S7007T was isolated by Dan-Dan Shang. Material preparation, experimental operation, data collection and analysis were performed by Dan-Dan Shang, He-Yuan Lun and Ke-Lei Zhu. The manuscript was written by Dan-Dan Shang. Project guidance and critical revision of manuscripts was performed by Guan-Jun Chen and Zong-Jun Du. All authors read and approved the final manuscript.

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Correspondence to Zong-Jun Du.

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This article does not contain any studies with animals performed by any of the authors. Informed consent was obtained from all individual participants included in the study.

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Communicated by Erko Stackebrandt.

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Shang, DD., Lun, HY., Zhu, KL. et al. Tenacibaculum pelagium sp. nov., isolated from marine sediment. Arch Microbiol 203, 2229–2236 (2021). https://doi.org/10.1007/s00203-021-02208-7

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