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Glycerol metabolism in a freeze-tolerant arctic insect: an in vivo13C NMR study

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Summary

Freeze-tolerance in larvae ofGynaephora groenlandica is enhanced by the accumulation of glycerol in the winter. Since summer larvae remain freeze-tolerant despite the lack of glycerol, we investigated glycerol metabolism as a function of acclimation and body temperature using non-invasive13C NMR spectroscopy. Major constituents of hemolymph isolated from cold- and warm-acclimated larvae were identified with the aid of standard NMR spectra and confirmed by TLC and GLC. Spectra obtained on live, warm-acclimated larvae showed the presence of lipids, glycogen, glucose, trehalose and amino acids. Similar spectra of cold-acclimated or previously frozen larvae showed the additional presence of glycerol. In vitro time-lapse13C spectra ofd-[1-13C]glucose added separately to hemolymph or extracted fat body tissue showed that glycerol is synthesized from glucose in the fat body tissue and distributed to the peripheral tissue via hemolymph. In vivo time-lapse13C spectra of cold- and warm-acclimated larvae were obtained after injection withd-[1-13C]glucose to monitor the production of labeled metabolic intermediates and end-products. [13C]Glycerol was produced between −30°C and 30°C but accumulated only below 5°C. Above 5°C glycerol was degraded and the13C label incorporated mainly into glycogen. The mechanism underlying temperature control of glycerol biosynthesis and degradation may provide a clue to the role of glycerol in enhancing freeze-tolerance in these insects.

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Authors and Affiliations

  1. Department of Biological Sciences, University of Notre Dame, 46556, Notre Dame, Indiana, USA

    Olga Kukal & John G. Duman

  2. Department of Chemistry, University of Notre Dame, 46556, Notre Dame, Indiana, USA

    Anthony S. Serianni

Authors
  1. Olga Kukal
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  2. Anthony S. Serianni
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  3. John G. Duman
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Kukal, O., Serianni, A.S. & Duman, J.G. Glycerol metabolism in a freeze-tolerant arctic insect: an in vivo13C NMR study. J Comp Physiol B 158, 175–183 (1988). https://doi.org/10.1007/BF01075831

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