Abstract
A photobioreactor was constructed using anchored polyurethane foam strips (1 x 1 x 40 cm) fixed onto a stainless-steel ring to prevent flotation, as a biomass support material (BSM). This type of reactor was named a seaweed-type bioreactor. A filamentous cyanobacterium, Scytonema sp. TISTR 8208, which produces a novel cyclic dodecapeptide antibiotic, was immobilized in seaweed-type photobioreactor and cultivated with air containing 5% CO2 sparged at a gas flow rate of 250 mL/min under illumination at a light intensity of 200 μmol photon m-2s-1. The antibiotic produced in the seaweed-type photobioreactor was purified by HPLC and examined regarding its spectrum and mode of action. The antibiotic effectively inhibited the growth of Gram-positive bacteria, pathogenic yeasts, and filamentous fungi, but it had only a weak effect on Gram-negative bacteria. Scanning electron micrograph analysis showed that the most characteristic change was swelling of the cells after exposure to the antibiotic. The antibiotic seems to alter the conformation of the microbial cell membrane, thereby changing its permeability, leading to osmotic shock.
Similar content being viewed by others
References
Glombitza, K.-W. and Koch, M. (1989), inAlgal and Cyanobacterial Biotechnology, Cresswell, R. C. and Shah, N. eds., Longman, Essex, p. 161–238.
Mason, C. P., Edwards, K. R., Carlson, R. E., Pignatello, J., Gleason, F. K., and Wood, J. M. (1982),Science 215, 400–402.
Pedersen, M. and DaSilva, E. J. (1973),Planta 115, 83–86.
Cardellina, J. H., II, Moore, R. E., Arnold, E. V., and Clardy, J. (1979),j. Org. Chem. 44, 4039–4042.
Kashiwagi, M., Mynderse, J. S., Moore, R. E., and Norton, T. R. (1980),j. Pharm. Sci. 69, 735–738.
Moore, R. E., Cheuk, C, and Patterson, G. M. L. (1984),j. Am. Chem. Soc. 106, 6456–6457.
Chetsumon, A., Fujieda, K., Hirata, K., Yagi, K., and Miura, Y. (1993),j. Appl. Phycol. 5, 615–622.
Chetsumon, A., Maeda, I, Umeda, U., Yagi, K., Miura, Y., and Mizoguchi, T. (1994),J. Appl. Phycol. 6, 539–543.
Chetsumon, A., Maeda, I., Umeda, F., Yagi, K., Miura, Y., and Mizoguchi, T. (1995),j. Appl. Phycol. 7, 135–139.
Keneman, E. W., Allen, S. D., Dowell, V. R., and Sommess, H. M. (1979), inColar Atlas and Textbook of Diagnostic Microbiology J. B. Lippincott, Philadelphia, p. 319–346.
Herman, C. and Donald, B. L. (1980), inAntibiotic in Laboratory Medicine, Lorian, V., eds, J. B. Lippincott, Philadelphia, p. 170–192.
Mallie, M., Butty, P., Montes, B., Jouvert, S., and Bastide, J.-M. (1991),Can. j. Microbiol. 37, 964–970.
Hamilton-Miller, J. M. T. (1973),Bacteriol. Rev. 37, 166–196.
Yamaguchi, H. (1978),Antimicrob. Agents Chemother. 13, 423–426.
Helms, G. L., Moore, R. E., Niemczura, W. P., Patterson, G. M. L., Tomer, K. B., and Gross, M. L. (1988),j. Org. Chem. 53, 1298–1307.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Chetsumon, A., Umeda, F., Maeda, I. et al. Broad spectrum and mode of action of an antibiotic produced byScytonema sp. TISTR 8208 in a seaweed-type bioreactor. Appl Biochem Biotechnol 70, 249–256 (1998). https://doi.org/10.1007/BF02920141
Issue Date:
DOI: https://doi.org/10.1007/BF02920141