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Coumarin in vanilla extracts: Its detection and significance

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Abstract

Coumarin was a component of some commercial vanilla preparations until banned in the United States by the Food and Drug Administration in 1954 because of evidence of hepatotoxicity in animals. However, in the present investigation coumarin was detected by capillary column gas chromatography/mass spectrometry in Mexican vanilla extracts commercially available or introduced in the United States by tourists or travelers. The significance of the presence of coumarin in Mexican vanilla extracts is discussed in light of recent evidence suggesting a lack of significant coumarin toxicity in humans.

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Literature Cited

  • Anonymous. 1906. Tinctura vanillini composite. The national formulary of unofficial preparations. 3rd ed, p. 187. American Pharmaceutical Assoc., Baltimore, MD.

    Google Scholar 

  • — 1985. Current prices of chemicals and related materials. Chem. Marketing Reporter 227: 50–58.

    Google Scholar 

  • Cohen, A. 1979. Critical review of the toxicology of coumarin with special reference to interspecies differences in metabolism and hepatotoxic response and their significance to man. Food Cosmet. Toxicol. 17:277–289.

    Article  PubMed  CAS  Google Scholar 

  • Courcelles, C, S. Rey, F. Carbonel, A. Dupont, M. Vandoorn, and P. Zelbstein. 1978. Vanille et produits “a goût de vanille”: un nouveau système de développement pour les chromatogrammes en couches minces. Ann. Falsif. Expert. Chim. 71:121–128.

    CAS  Google Scholar 

  • Feeny, F. J. 1964. Determination of vanillin by ultraviolet absorption. J. Assoc. Off. Agric. Chem. 47:555–557.

    CAS  Google Scholar 

  • Galetto, W. G., and P. G. Hoffman. 1978. Some benzyl ethers present in the extract of Vanilla (Vanilla planifolia). J. Agric. Food Chem. 26:195–197.

    Article  CAS  Google Scholar 

  • Hernández, F. 1651. Rerum medicarum Novae Hispaniae thesaurus seu plantarum, animalium, mineralium, Mexicanorum historia, p. 38. Typographeio Vitalis Mascardi, Rome.

    Google Scholar 

  • Herrmann, A., and M. Stöckli. 1982. Rapid control of vanilla-containing products using highperformance liquid chromatography. J. Chromatogr. 246:313–316.

    Article  CAS  Google Scholar 

  • Hopkins, H. 1983. Mexican vanilla no bargain. FDA Consumer, October 1983:11.

  • Jürgens, U. 1981. Zur hochdruckflüssigchromatographischen Analyse von Aromen: Untersuchung von Grundstoffen und Getränken sowie von Vanille- und Vanillin-Zuckern in Kleinpackungen. Deutsche Lebensmut.-Rundschau 77:211–213.

    Google Scholar 

  • Kahan, S., and J. Fitelson. 1964. Chromatographie detection of flavor additives in vanilla extract. J. Assoc. Off. Agric. Chem. 47:551–555.

    CAS  Google Scholar 

  • Lake, B. G. 1984. Investigations into the mechanism of coumarin-induced hepatotoxicity in the rat. Arch. Toxicol., Suppl. 7:16–29.

    CAS  Google Scholar 

  • Magida, P. 1984. Mexican vanilla poses unsafe risk of poison. Chicago Tribune, 21 March, sect. 7, p. 4B.

  • Martin, G. E., J. F. Feeny, and F. P. Scaringelli. 1964. Determination of vanillin and ethyl vanillin by gas-liquid chromatography. J. Assoc. Off. Agric. Chem. 47:561–562.

    CAS  Google Scholar 

  • Merat, E., and J. Vogel. 1976. Quelques considérations sur les méthodes spectrometriques de dosage des constituants d’arômes de vanille. Mitt. Geb. Lebensmitteluntersuch. Hyg. 67:438–447.

    CAS  Google Scholar 

  • O’Reilly, R. 1980. Anticoagulant, antithrombotic, and thrombolytic drugs.In A. G. Gilman, L. Goodman, and A. Gilman, eds., The pharmacological basis of therapeutics, 6th ed, p. 1353. Macmillan, New York.

    Google Scholar 

  • Rother, R. 1885. Tincture of vanilla. Amer. J. Pharm. October 1885:500-502.

    Google Scholar 

  • Schlack, J., and J. Dicecco. 1974. Gas-liquid Chromatographie quantitative determination of vanillin and ethyl vanillin in foods. J. Assoc. Off. Agric. Chem. 57:329–331.

    CAS  Google Scholar 

  • Sengupta, P., A. R. Sen, A. Bose, and T. V. Mathew. 1973. Detection of coumarin in vanilla flavoured food by TLC. Res. & Industr. (New Delhi) 18:96–97.

    CAS  Google Scholar 

  • Sullivan, G. 1981a. Examination of Mexican vanilla extracts for coumarin adulteration: Part I. Veterin. Human Toxicol. 23:89–91.

    CAS  Google Scholar 

  • — 1981b. Examination of Mexican vanilla extracts for coumarin adulteration: Part II. Veterin. Human Toxicol. 23:161–163.

    CAS  Google Scholar 

  • — 1981c. Examination of Mexican vanilla extracts for coumarin adulteration: Part III-Quantitative determination of coumarin. Veterin. Human Toxicol. 23:249–251.

    CAS  Google Scholar 

  • — 1982. Occurrence of umbelliferone in the seeds ofDipteryx odorata (Aubl.) Willd. J. Agric. Food Chem. 30:609–610.

    Article  CAS  Google Scholar 

  • — 1984. Major aromatic constituents in Mexican vanilla extracts. Texas J. Sei. 36:17-23. Vande Casteele, K., H. Geiger, and C. Van Sumere. 1983. Separation of phenolics (benzoic acids, cinnamic acids, phenylacetic acids, quinic acid esters, benzaldehydes and acetophenones, miscellaneous phenolics) and coumarins by reversed-phase high-performance liquid chromatography. J. Chromatogr. 258:111–124.

    Google Scholar 

  • Vanhaelen, M., and R. Vanhaelen-Fastré. 1980. High-performance liquid, gas-liquid and thin-layer chromatography of naturally occurring flavonoids, phenolics, and related compounds. J. Chromatogr. 187:255–260.

    Article  CAS  Google Scholar 

  • Ximenez, F. 1615. Cuatro libros de la naturaleza, y virtudes de las plantas, p. 33–35. Casa de la Viuda de Diego López Daualos, Mexico.

    Google Scholar 

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Marles, R.J., Compadre, C.M. & Farnsworth, N.R. Coumarin in vanilla extracts: Its detection and significance. Econ Bot 41, 41–47 (1987). https://doi.org/10.1007/BF02859345

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  • DOI: https://doi.org/10.1007/BF02859345

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