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Drug transport across the blood — brain barrier

II. Experimental techniques to study drug transport

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Abstract

This is part II of a review on the transport of drugs across the blood-brain barrier. In this part, the emphasis is on the various experimental techniques that can be used to characterize the blood — brain barrier transport of drugs. Generally speaking, three approaches can be distinguished:in vitro techniques using isolated brain capillaries, cerebrovascular endothelial cells in primary culture or endothelium-derived cell lines;in vivo techniques (both single-passage and multi-passage techniques) andin situ perfusion techniques. Each of these techniques has specific advantages and disadvantages associated with it. Therefore, in many instances, a combination of different approaches is needed to study the fundamental aspects of drug transport across the blood-brain barrier.

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Authors and Affiliations

  1. Centre for Bio-Pharmaceutical Sciences, Division of Pharmacology, Leiden University, Sylvius Laboratories, P.O. Box 9503, 2300, RA Leiden, the Netherlands

    J. B. M. M. Van Bree, A. G. De Boer, M. Danhof & D. D. Breimer

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  1. J. B. M. M. Van Bree
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  2. A. G. De Boer
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  3. M. Danhof
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  4. D. D. Breimer
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Van Bree, J.B.M.M., De Boer, A.G., Danhof, M. et al. Drug transport across the blood — brain barrier. Pharmaceutisch Weekblad Scientific Edition 14, 338–348 (1992). https://doi.org/10.1007/BF01970169

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