Summary
Phase contrast and electron microscopical investigations on the gill ofSepia officinalis L., from the stage shortly before hatching to the adult stage, demonstrate (1) peripheral respiratory epithelial areas and (2) highly folded epithelia rich in mitochondria in the concave recesses of the gill lamellae. Enzyme-histochemical and cytochemical findings, and in particular the GOT proof, suggest by analogy with the chloride cells of the teleosts that the cells of this second type of epithelium are capable of active transport, and that they probably secrete ammonia. Apparently the gill of cephalopods, too, serves both respiratory and excretory functions.
References
Boletzky, S. von: Effets de la sous-nutrition prolongée sur le développement de la coquille deSepia officinalis L. (Mollusca, Cephalopoda). Bull. Soc. Zool. France99, 667–673 (1974)
Burck, H.C.: Histologische Technik. Stuttgart: Thieme-Verlag, 1973
Burstone, M.S.: New histochemical technique for the demonstration of tissue oxidase (cytochrome oxidase). J. Histochem. Cytochem.7, 112–122 (1959)
Cuvier, G.: Le règne animal. Les céphalopodes: Paris 1817
Deimling, O.H. von: Die Darstellung phosphatfreisetzender Enzyme mittels Schwermetall-Simultanmethode. Histochemie4, 48–55 (1964)
Donaubauer, H.H., Schipp, R.: Localization of enzyme activities in the pancreatic appendages ofSepia officinalis L. (Cephalopoda). Histochemistry59, 29–44 (1978)
Glenner, G.G., Burtner, H.J., Brown, G.W.: The histochemical demonstration of monoaminoxidase activity by tetrazolium salts. J. Histochem. Cytochem.5, 591–600 (1957)
Goebel, A., Puchtler, H.: Zur Darstellung der Carboanhydrase im histologischen Schnitt. Naturwissenschaften41, 531–532 (1954b)
Gomori, G.: An improved histochemical technique for acid phosphatase. Stain Technol.25, 81–85 (1950)
Gomori, G.: The complex nature of alkaline phosphatase. Biochem. biophys. Acta (Amst.)8, 162–172 (1952)
Joubin, L.: Structure et développement de la branchie de quelques Céphalopodes des côtes de France. Arch. Zool. exp. gén. (2e série),3, 75–150 (1885)
Karnovsky, M.J., Himmelhoch, S.R.: Histochemical localization of glutaminase I activity in kidney. Am. J. Physiol.201, 786–790 (1961)
Lee, Sin Hang: The possible role of the vesicles in renal ammonia excretion. J. of Cell Biol.45, 644–649 (1970)
Lolova, I., Dikov, A.: Histochemical evidence of aminotransferase. IV. Histochemical and electrophoretical investigation of aminotransferase in rat organs. Acta Histochem.53, 12–27 (1975)
Magnun, C.P., Dykens, J.A., Henry, R.P., Polites, G.E.: The excretion of NH +4 and its ouabain sensitivity in aquatic annelids and molluscs (1). J. Exp. Zool.203, 151–159 (1978)
Newstead, J.D.: Fine structure of the respiratory lamellae of teleostean gills. Z. Zellforschung79, 396–428 (1967)
Pearse, A.G.E.: Histochemistry. Theoretical and applied. 2nd and 3rd Ed. London: Churchill 1961/1968
Philpott, C.W. and Copeland, D.E.: Fine structure of chloride cells from three species ofFundulus. J. Cell Biol.18, 389 (1963)
Potts, W.T.W.: Ammonia excretion inOctopus dofleini. Comp. Biochem. Physiol.16, 479–489 (1965)
Robertson, J.D.: Further studies on ionic regulation in marine invertebrates. J. Exp. Biol.30, 277–296 (1953)
Sasse, D.: Glykogen in der Ontogenese des Verdauungstraktes — Chemomorphologische und stoffwechselhistochemische Analyse. Ergebn. Anat. Entwick.-Gesch.40/2, 1–68 (1968)
Schäfer, P.: Über die Atmungsorgane der tetra- und dibranchiaten Cephalopoden. Inaug. Diss. Leipzig (1904)
Schäfer, A., Schipp, R.: Vergleichende elektronenmikroskopische Untersuchungen an den Kiemen verschiedener Teleostier zur osmoregulatorischen Funktion der Chloridzellen. Verh. der Anat. Ges. Leipzig 1968, Anat. Anz.125, 277–289 (1969)
Schipp, R., Schäfer, A.: Die Besonderheiten der Feinstruktur von Kiemenherz und Kiemengefäßen von Cephalopoden. Verh. der Deutsch. Zool. Ges. in Innsbruck (1968)
Schipp, R., Höhn, P., Schäfer, A.: Elektronenmikroskopische und histochemische Untersuchungen zur Funktion des Kiemenherzanhangs (Pericardialdrüse) von Sepia officinalis. Z. Zellforschung117, 252–274 (1971)
Schipp, R., Boletzky, S. von: Morphology and function of the excretory organs in dibranchiate Cephalopods. Fortschritte der Zoologie23, 2/3 (1975)
Schipp, R., Boletzky, S. von, Doell, G.: Ultrastructural and cytochemical investigations on the renal appendages and their concrements in dibranchiate cephalopods (Mollusca, Cephalopoda). Z. Morph. Tiere81, 279–304 (1975)
Schoffeniels, E.: Isolation of a sodium-dependent ATPase from the gills ofOctopus vulgaris L. Life Sciences9, 437–440 (1962)
Seligman, A.M., Kwang-Chung Tson, Rutenberg, S.H., Cohen, R.B.: Histochemical demonstration ofβ-D-glucuronidase with a synthetic substrate. J. Histochem. Cytochem.2, 209–229 (1954)
Tilesius von Tillenau, W.G.: De respirationeSepia officinalis. Diss. Univ. Lipsiae, 88 pp (1861)
Tompsett, D.H.: Sepia. Univ. Press of Liverpool 1939
Towle, D.W., Gilman, M.E., Hempel, J.D.: Rapid modulation of gill Na++K+-dependent ATPase activity during acclimation of the killifishFundulus heteroclitus to salinity change. J. Exp. Zool.202, 2, 179–213 (1977)
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This study was supported by the Deutsche Forschungsgemeinschaft
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Schipp, R., Mollenhauer, S. & von Boletzky, S. Electron microscopical and histochemical studies of differentiation and function of the cephalopod gill (Sepia officinalis L.). Zoomorphologie 93, 193–207 (1979). https://doi.org/10.1007/BF00993999
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DOI: https://doi.org/10.1007/BF00993999