Abstract
Cuprizone affects the liver of treated mice in a random manner, causing no appreciable change in some cases and inducing the formation of megamito-chondria with altered properties in others. Lack of a full appreciation of this variability may be at the origin of some discrepancies in published work dealing with the properties of cuprizone mouse liver mitochondria (CMLM). CMLM from fully affected livers were remarkably labile and difficult to isolate in a coupled state by homogenization and centrifugation techniques. The integral respiratory chain proteins of CMLM were functionally normal, with the exception of succinic dehydrogenase which showed considerable inhibition. Coupled morphological and functional analysis provided evidence that these properties were independent of CMLM size, a matter which had remained doubtful thus far and bears on the validity of literature reports.
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Abbreviations
- MLM:
-
mouse liver mitochondria
- CMLM:
-
cuprizone mouse liver mitochondria
- PMS:
-
phenazine methosulfate
- Mops:
-
4-morphoinopropanesulfonic acid
- TMPD:
-
tetramethyl-p-phenylenediamine
- AA:
-
Antimycin A
- RCR:
-
respiratory control ratio
- BSA:
-
bovine serum albumin
References
Amchenkova, A. A., Bakeeva, L. E., Chentsov, Y. S., Skulachev, V. P., and Zorov, D. B. (1988).J. Cell Biol. 107, 481–495.
Campo, M. L., and Tedeschi, H. (1984).Eur. J. Biochem. 141, 5–7.
Campo, M. L., Bowman, C. L., and Tedeschi, H. (1984).Eur. J. Biochem. 141, 1–4.
Flatmark, T., Kryvi, H., and Tangeras, A. (1980).Eur. J. Cell. Biol. 23, 141–148.
Folch, J., Lees, M., and Sloane Stanley, G. H. (1957).J. Biol. Chem. 226, 497–509.
Groen, A. K., Wanders, R. J. A., Westerhoff, H. V., Van der Meer, R., and Tager, J. (1982).J. Biol. Chem. 257, 2754–2757.
Gupte, S., Wu, E.-S., Hoechli, L., Hoechli, M., Jacobson, K., Sowers, A. E., and Hackenbrock, C. R. (1984).Proc. Natl. Acad. Sci. USA 81, 2606–2610.
Hochman, J. H., Schindler, M., Lee, J. G., and Ferguson-Miller, S. (1982).Proc. Natl. Acad. Sci. USA 79, 6866–6870.
Hochman, J., Ferguson-Miller, S., and Schindler, M. (1985).Biochemistry 24, 2509–2516.
Hoppel, C. L., and Tandler, B. (1973).Biochem. Pharmacol. 22, 2311–2318.
Hoppel, C. L., and Tandler, B. (1975).J. Nutrition 105, 562–570.
Kimura, T., Hauber, J., and Singer, T. P. (1967).J. Biol. Chem. 242, 4987–4993.
Kinnally, K. W., Campo, M. L., and Tedeschi, H. (1989a).J. Bioenerg. Biomembr. 21, 497–506.
Kinally, K., Tedeschi, H., Mannella, C. A., and Frisch, H. L. (1989b).Biophys. J. 55, 1205–1213.
Kroeger, A., and Klingenberg, M. (1973).Eur. J. Biochem. 39, 313–323.
Loud, A. V. (1968).J. Cell. Biol. 37, 27–46.
Loud, A. V., Barany, W. C., and Pack, B. A. (1965).Lab. Invest. 14, 996–1008.
Maloff, B. L., Scordilis, S. P., Reynolds, C., and Tedeschi, H. (1978a).J. Cell. Biol. 78, 199–213.
Maloff, B. L., Scordilis, S. P., and Tedeschi, H. (1978b).J. Cell. Biol. 78, 214–226.
Munn, E. A. (1974). ‘The Structure of Mitochondria’, Academic Press, London, New York.
Pellegrini, M. (1980).J. Cell Sci. 46, 137–166.
Sorgato, M. C., Keller, B. U., and Stuehmer, W. (1987).Nature (London)330, 498–500.
Sorgato, M. C., Moran, O., De Pinto, V., Keller, B. U., and Stuehmer, W. (1989).J. Bioenerg. Biomembr. 21, 485–496.
Suchy, J., and Cooper, C. (1974).Exp. Cell Res. 88, 198–202.
Suzuki, K. (1969).Science 163, 81–82.
Tandler, B., and Hoppel, C. L. (1973).J. Cell Biol. 56, 266–272.
Tandler, B., and Hoppel, C. L. (1975).Beitr. Pathol. 156, 56–64.
Tandler, B., and Hoppel, C. L. (1986).Ann. N.Y. Acad. Sci. 488, 65–81.
Tedeschi, H., and Kinnally, K. W. (1987).J. Bioenerg. Biomembr. 19, 321–327.
Tedeschi, H., Mannella, C. A., and Bowman, C. L. (1987).J. Membr. Biol. 97, 21–29.
Terada, H., and Van Dam, K. (1975).Biochim. Biophys. Acta 387, 507–518.
Tupper, J., and Tedeschi, H. (1969a).Proc. Natl. Acad. Sci. USA 63, 370–377.
Tupper, J. and Tedeschi, H. (1969b).Procl. Natl. Acad. Sci. USA 63, 713–717.
Veeger, C., Der Vartanian, D. V., and Zeylemaker, W. P. (1969).Methods Enzymol. 13, 81–90.
Wagner, T., and Rafael, J. (1975).Biochim. Biophys. Acta 408, 284–296.
Wagner, T., and Rafael, J. (1977).Exp. Cell Res. 107, 1–13.
Wakabayashi, T., Asano, M., and Kurono, C. (1974).J. Electron Microsc. 23, 247–254.
Wakabayashi, T., Asano, M., and Kurono, C. (1975a).Acta Pathol. Jpn. 25, 15–37.
Wakabayashi, T., Asano, M., Kurono, C., and Ozawa, T. (1975b).Acta Pathol. Jpn. 25, 39–49.
Wakabayashi, T., Asano, M., Ishikawa, K., and Kishimoto, H. (1977).J. Electron Microsc. 26, 137–140.
Wakabayashi, T., Asano, M., Ishikawa, K., and Kishimoto, H. (1978).Acta Pathol. Jpn. 28, 215–223.
Wakabayashi, T., Horiuchi, M., Kawamoto, S. and Onda, H. (1984).Acta Pathol. Jpn. 34, 481–488.
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Petronilli, V., Zoratti, M. A characterization of cuprizone-induced giant mouse liver mitochondria. J Bioenerg Biomembr 22, 663–677 (1990). https://doi.org/10.1007/BF00809070
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DOI: https://doi.org/10.1007/BF00809070