Summary
L-alanine was produced continuously from fumaric acid by means of soluble aspartase and L-aspartate-β-decarboxylase. The two reaction steps were carried out in two membrane reactors in series at different pH and temperature. The retention of the soluble enzymes within the reactor vessels was achieved by means of ultrafiltration membranes.
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Jandel, AS., Hustedt, H. & Wandrey, C. Continuous production of L-alanine from fumarate in a two-stage membrane reactor. European J. Appl. Microbiol. Biotechnol. 15, 59–63 (1982). https://doi.org/10.1007/BF00499507
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DOI: https://doi.org/10.1007/BF00499507