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A simple fluorescence technique to stain the plasma membrane of human neutrophils

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Summary

Three different fluorochromes were tested for their ability to label the plasma membrane proteins of neutrophils without labelling intracellular structures. A fluorescence quenching technique was used to differentiate between extra- and intracellularly localized fluorescence. Fluorescamin and fluoresceinisothiocyanate were shown to stain intracellular structures as well as the plasma membranes of the cells. Another fluorochrome, Evans Blue, is proposed since this dye was shown, by using the fluorescence quenching technique, to selectively stain the plasma membrane of viable neutrophils.

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References

  • Butcher EC, Weissman IL (1980) Direct fluorescent labelling of cells with fluorescein or rhodamin isothiocyanate. I. Technical aspects. J Immunol Methods 37:97–198

    Google Scholar 

  • Dahlgren C, Magnusson K-E, Rundquist I, Stendahl O (1980) Modulation of polymorphonuclear leukocyte locomotion by synthetic amphiphils. Cell Biophys 2:253–267

    Google Scholar 

  • Enerbäck L, Rundquist I (1981) DNA distribution of mast cell populations in growing rats. Histochemistry 71:521–531

    Google Scholar 

  • Hed J (1977) The extinction of fluorescence by crystal violet and its use to differentiate between attached and ingested microorganisms in phagocytosis. FEBS Lett 1:357–361

    Google Scholar 

  • Hed J, Stendahl O (1982) Differences in the ingestion mechanisms of IgG and C3b particles in phagocytosis by neutrophils. Immunology 45:727–736

    Google Scholar 

  • Ryan GB, Borysenko JZ, Karnovsky MJ (1974) Factors affecting the redistribution of surfacebound concanavalin A on human polymorphonuclear leukocytes. J Cell Biol 62:351–365

    Google Scholar 

  • Sahlin S, Hed J, Rundquist I (1983) Differentiation between attached and ingested immune complexes by a fluorescence quenching cytofluorometric assay. J Immunol Methods 60:115–124

    Google Scholar 

  • Segal AM, Peters TS (1977) Analytical subcellular fractionation of human granulocytes with special reference to the localization of enzymes involved in microbicidal mechanisms. Clin Sci Mol Med 52:429–442

    Google Scholar 

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Authors and Affiliations

  1. Department of Medical Microbiology, University of Linköping, S-581 85, Linköping, Sweden

    J. Hed & C. Dahlgren

  2. Department of Pathology II, University of Linköping, S-581 85, Linköping, Sweden

    I. Rundquist

Authors
  1. J. Hed
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  2. C. Dahlgren
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  3. I. Rundquist
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Hed, J., Dahlgren, C. & Rundquist, I. A simple fluorescence technique to stain the plasma membrane of human neutrophils. Histochemistry 79, 105–110 (1983). https://doi.org/10.1007/BF00494347

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  • DOI: https://doi.org/10.1007/BF00494347

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