Summary
A strain of Pestalotia rhododendri Guba produced good growth in a simple glucose-mineral-salt medium with the addition of thiamine.
The fungus was grown on agar medium in Ryan-tubes and through some of the tubes an airstream was passed. For optimal growth an airflow rate of at least 60 ml/min was necessary.
The growth-promoting effect of aeration was studied in relation to pH of the medium, relative humidity, oxygen supply, carbon dioxide accumulation and various nitrogen sources.
No volatile, organic metabolite stimulating the growth was found.
On a medium with a low carbon and a high nitrogen content, aeration made growth of Pestalotia possible when nonaerated mycelia were nearly completely inhibited. In an unbuffered medium accumulation of ammonia could explain the inhibition of growth.
The agar or impurities in the agar contributed to the nitrogen nutrition of the fungus.
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Norrman, J., Fries, N. The growth of pestalotia rhododendri Guba in relation to volatile metabolites. Archiv. Mikrobiol. 56, 330–343 (1967). https://doi.org/10.1007/BF00425208
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DOI: https://doi.org/10.1007/BF00425208