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Immunochemical studies on barley seed storage proteins

The specificity of an antibody to ‘C’ hordein and its reaction with prolamins from other cereals

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Abstract

The antigenic relationships between the prolamins of barley, rye and wheat have been studied by examining the specificity of an antibody to ‘C’ hordein in a quantitative study using a laser nephelometer. The antibody reacts weakly with ‘B’ hordein and strongly with 75-kdalton and 40-kdalton γ-secalins from rye and γ3 some ω-gliadins from wheat. Absorption experiments and immunodiffusion tests indicate that there are shared antigenic determinants for most of the prolamins. All the species with reacting prolamins belong to the sub-family Festucoideae of the Gramineae. The prolamins of maize, pearl millet and sorghum, species of the sub-family Panicoideae, do not react. The results confirm the known lack of homology between the prolamins of the two sub-families and also indicate the presence of relationships not yet established between ‘C’ hordein, the 75-kdalton and 40-kdalton γ-secalins and also γ3 gliadin.

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Abbreviations

HMW:

high molecular weight

PAGE:

polyacnylamide-gel electrophoresis

PBS:

phosphate-buffered saline

RLS:

relative light scattering

SDS:

sodlum dodecyl sulphate

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Authors and Affiliations

  1. Biochemistry Department, Rothamsted Experimental Station, AL5 2JQ, Harpenden, Herts, UK

    Gerald N. Festenstein, Benjamin J. Miflin & Peter R. Shewry

  2. Immunology Department, Middlesex Hospital Medical School, London, UK

    Frank C. Hay

Authors
  1. Gerald N. Festenstein
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  2. Frank C. Hay
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  3. Benjamin J. Miflin
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  4. Peter R. Shewry
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Festenstein, G.N., Hay, F.C., Miflin, B.J. et al. Immunochemical studies on barley seed storage proteins. Planta 162, 524–531 (1984). https://doi.org/10.1007/BF00399918

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  • DOI: https://doi.org/10.1007/BF00399918

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