Abstract
Cell-free extracts from Rhus typhina L. (staghorn sumach) leaves were found to catalyze the transfer of the galloyl moiety of β-glucogallin (1-O-galloyl-β-D-glucose) to 1,6-di-O-galloyl-β-D-glucose, resulting in the specific formation of 1,2,6-tri-O-galloyl-β-D-glucose, an intermediate of gallotannin biosynthesis. The reaction product was unequivocally identified by co-chromatography with authentic references using reversed-phase high-performance liquid chromatography and by 1H-nuclear-magnetic-resonance spectroscopy.
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Abbreviations
- HPLC:
-
high-performance liquid chromatography
- NMR:
-
nuclear magnetic resonance
References
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Denzel, K., Schilling, G. & Gross, G.G. Biosynthesis of gallotannins. Enzymatic conversion of 1,6-digalloylglucose to 1,2,6-trigalloylglucose. Planta 176, 135–137 (1988). https://doi.org/10.1007/BF00392489
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DOI: https://doi.org/10.1007/BF00392489