Abstract
Leaf discs from etiolated bean plants were found to incorporate [3H]lysine into 80 S ribosomesynthesized proteins in the presence of chloramphenicol (100 mg l−1) when exposed to light. After a 7 min pulse of [3H]lysine, the discs were transferred to the same medium but with nonradioactive lysine, and postincubation was carried out for 24 h. The number of silver grains over the plastids, after the first period of a lag phase, indicates a large increase between 12 and 24 h of postincubation. Simultaneously, the labeling of the cytoplasm becomes reduced during that period. The results show that during inhibition of the protein formation within plastids, the synthesis of plastid-destined proteins in cytoplasm, as well as their transport into plastids, can still proceed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Blobel, G., Dobberstein, B.: Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains of membrane bound ribosomes of murine myeloma. J. Cell. Biol. 67, 835–851 (1975)
Campbell, P.N., Blobel, G.: The role of organelles in the chemical modification of the primary translation products of secretory proteins. FEBS Lett. 72, 215–226 (1976)
Cobb, A.H., Wellburn, A.R.: Developmental changes in the levels of SDS-extractable polypeptides during plastid morphogenesis. Planta 114, 131–142 (1973)
Cobb, A.H., Wellburn, A.R.: Changes in plastid envelope polypeptides during chloroplast development. Planta 121, 273–282 (1974)
Cobb, A.H., Wellburn, A.R.: Polypeptide binding to plastid envelopes during chloroplast development. Planta 129, 127–132 (1976)
Cockburn, B.J., Wellburn, A.R.: Changes in the envelope permeability of developing chloroplasts. J. Exp. Bot. 25, 36–49 (1974)
Ellis, R.J.: Protein and nucleic acid synthesis by chloroplasts. In: Topics in photosynthesis, vol. I: The intact chloroplast, pp. 335–364, Barber, J., ed. Amsterdam: Elsevier 1976
Feierabend, J., Mikus, M.: Occurence of a high temperature sensitivity of chloroplast ribosome formation in several higher plants. Plant Physiol. 59, 863–867 (1977)
Feierabend, J., Schrader-Reichhardt, U.: Biochemical differnetiation of plastids and other organelles in rye leaves with a high-temperature-induced deficiency of plastid ribosomes. Planta 129, 133–145 (1976)
Feierabend J., Wildner, G.: Formation of the small subunit in the absence of the large subunit of ribulose-1,5-bisphosphate carboxylase in 70 S ribosome deficient rye leaves. Arch. Biochem. Biophys. 186, 283–291 (1978)
Gooding, L.R., Roy, H., Jagendorf, A.T.: Immunological identification of nascent subunits of wheat ribulose diphosphate carboxylase on ribosomes of both chloroplast and cytoplasmic origin. Arch. Biochem. Biophys. 159, 324–335 (1973)
Hampp, R., Schmidt, H.W.: Changes in envelope permeability during chloroplast development. Planta 129, 69–74 (1976)
Hampp, R., Wellburn, A.R.: Early changes in envelope permeability of developing chloroplasts J. Exp. Bot. 27, 778–784 (1976)
Highfield, P.E., Ellis, R.J.: Synthesis and transport of the small subunit of chloroplast ribulose bisphosphate carboxylase. Nature 271, 420–424 (1978)
Hoober, K.J., Stegeman, W.J.: Control of the synthesis of a major polypeptide of chloroplast membranes in Chlamydomonas reinhardii. J Cell. Biol. 56, 1–12 (1973)
Kirk, J.T.O., Tilney-Bassett, R.A.E.: The plastids. London, San Francisco: Freeman 1967
Laulhere, J.P., Dorne, A.M.: Are cytoplasmic ribosomes in chloroplast preparations functionally attached to the chloroplast membrane? Plant Sci. Lett 8, 251–256 (1977)
Schäfers, H.A., Feierabend, J.: Ultrastructural differentiation of plastids and other organelles in rye leaves with high-temperature induced deficiency of plastid ribosomes. Cytobiologie 14, 75–90 (1976)
Strzałka, K., Majewska, G., Mędrela, E.: Effects of chloamphenicol and cycloheximide on the relative contents of chlorophyll and protein in various subchloroplast fractions. Physiol. Plant. (in press)
Walles, B.: Plastid inheritance and mutations. In: Structure and function of chloroplasts, pp. 51–88. Gibbs, M., ed. Berlin, Heidelberg, New York: Springer 1971
Woodcock, C.L.F., Bogorad, L.: Nucleic acids and information processing in chloroplasts. In: Structure and function of chloroplasts, pp. 89–128. Gibbs, M., ed. Berlin, Heidelberg, New York: Springer 1971
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Strzałka, K., Kwiatkowska, M. Transport of proteins from cytoplasm into plastids in chloramphenicol-treated bean leaf discs. Planta 146, 393–398 (1979). https://doi.org/10.1007/BF00380850
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00380850