Summary
This report describes a comparison of the efficiency of transduction of genes in E. coli by the generalized transducing bacteriophages T4GT7 and P1CM. Both phages are capable of transducing many genetic markers in E. coli although the frequency of transduction for particular genes varies over a wide range. The frequency of transduction for most genes depends on which transducing phage is used as well as on the donor and recipient bacterial strains. Analysis of T4GT7 phage lysates by cesium chloride density gradient centrifugation shows that transducing phage particles contain primarily bacterial DNA and carry little, if any, phage DNA. In this regard transducing phages P1CM and T4GT7 are similar; both phages package either bacterial or phage DNA but not both DNAs into the same particle.
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References
Chelala CA, Margolin P (1974) Effects of deletions on co-transduction linkage in Salmonella typhimurium. Mol Gen Genet 131:97–112
Drexler H (1970) Transduction by bactriophage T1. Proc Natl Acad Sci USA 66:1083–1088
Ikeda H, Tomizawa JI (1965) Transducing fragments in generalized transduction by phage P1. J Mol Biol 14:85–109
Jackson EN, Laski F, Andres C (1982) Bacteriophage P22 mutants that alter the specificity of DNA packaging. J Mol Biol 154:551–564
Koerner JF, Snustad DP (1979) Shut-off of host macromolecular synthesis after T-even bacteriophage infection. Microbiol Rev 43:199–223
Kondo E, Mitsuhashi S (1964) Drug resistance of enteric bacteria. IV. Active transducing bacteriophage P1CM produced by the combination of R-factor with bacteriophages P1. J Bacteriol 88:1266–1276
Laski F, Jackson EN (1982) Maturation cleavage of bacteriophage P22 DNA in the absence of DNA packaging. J Mol Biol 154:565–579
Lennox ES (1955) Transduction of linked genetic characters of the host by bacteriophage P1. Virology 1:190–206
Luria SE, Adams JN, Ting RC (1960) Tranduction of lactose utilizing ability among strains of E. coli and S. dysenteriae and the properties of the transducing phage particles. Virology 12:348–390
Masters M (1977a) The frequency of P1 transduction of the genes of Escherichia coli as a function of chromosomal position: Preferential transduction of the origin of replication. Mol Gen Genet 155:197–202
Masters M (1977b) P1 transduction frequencies: A clue to chromosome structure? In: Molineux I, Kohijama M (eds) DNA synthesis. Plenum, New York, p 431
Miller J (1972) In: Experiments in Molecular Genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor NY
Morton D, Kutter EM, Guttman BS (1978) Synthesis of T4 DNA and bacteriophage in the absence of dCMP hydroxymethylase. J Virol 28:262–269
Newman BJ, Masters M (1980) The variation in frequency with which markers are transduced by phage P1 is primarily a result of discrimination during recombination. Mol Gen Genet 180:585–589
Ramsey N, Ritchie DA (1983) Uncoupling of initiation site cleavage from subsequent headful cleavages in bacteriophage T1 DNA packaging. Nature (London) 301:264–266
Schmieger H (1970) The molecular structure of the transducing particles of Salmonella phage P22. Mol Gen Genet 109:323–337
Schmieger H (1972) Phage P22 mutants with increased or decreased transduction abilities. Mol Gen Genet 119:75–88
Schmieger H, Buck U (1975) Apperance of transducing particles and the fate of host DNA after infection of Salmonella typhimurium with P22-mutants with increased transducing ability (HT-mutants)
Snustad DP, Bursch CJH, Hefeneider SH (1976) Mutants of bacteriophage T4 deficient in the ability to induce nuclear disruption. J Virol 18:268–288
Streisinger G, Emrich J, Stahl MM (1967) Chromosome structure in phage T4, terminal redundancy and length determination. Proc Natl Acad Sci USA 57:292–295
Susskind MM, Botstein D (1978) Molecular genetics of bacteriophage P22. Mirobiol Rev 42:385–413
Tye BK, Huberman JA, Botstein DJ (1974) Non-random permutation of phage P22 DNA. J Mol Biol 85:501–532
Weaver HR, Snustad DP, Jorgenson SE, Koerner JG (1970) Isolation of T4 mutants defective in the ability to degrade host deoxyribonucleic acid. J Virol 5:700–705
Wilson GG, Young KKY, Edlin GJ, Konigsberg W (1979) High frequency generalized transduction by bacteriophage T4. Nature (London) 280:80–82
Young KKY, Edlin GJ, Wilson GG (1982) Genetic analysis of bacteriophage T4 transducing bacteriophages. J Virol 41:345–347
Zinder ND, Lederberg J (1952) Genetic exchange in Salmonella. J Bacteriol 64:679–699
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Communicated by G. O'Donovan
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Young, K.K.Y., Edlin, G. Physical and genetical analysis of bacteriophage T4 generalized transduction. Molec Gen Genet 192, 241–246 (1983). https://doi.org/10.1007/BF00327673
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DOI: https://doi.org/10.1007/BF00327673