Abstract
We have used the fluorescence recovery after photobleaching technique to study the translational diffusion, in Lα phase multibilayers of 1-palmitoyl-2-oleoylphosphatidylcholine (POPC), of fluorescent derivatives of 1-palmitoyl-2-oleoylphosphatidylethanolamine (NBD-POPE) and a membrane-spanning phosphatidylethanolamine (NBD-MSPE). The latter derivative was prepared from a membrane-spanning glycerol-dialkyl-glycerol tetraether lipid isolated from the thermophilic and acidophilic archaebacterium Sulfolobus solfataricus. The translational diffusion was examined between about 15° and 45°C. It is shown that over this temperature range the translational diffusion coefficient for NBD-MSPE is ∼2/3 that for NBD-POPE which spans only one monolayer of the bilayer. The result is interpreted in terms of existing models for translational diffusion in lipid membranes.
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Abbreviations
- D t :
-
translational diffusion coefficient
- FRAP:
-
fluorescence recovery after photobleaching
- MSPE:
-
a membrane-spanning phosphatidylethanolamine derived from a glycerol-dialkyl-glycerol tetraether lipid isolated from Sulfolobus solfataricus
- NBD:
-
4-nitrobenz-2-oxa-1,3-diazolyl
- PE:
-
phosphatidylethanolamine
- POPC:
-
1-palmitoyl-2-oleoylphosphatidylcholine
- POPE:
-
1-palmitoyl-2-oleoylphosphatidylethanolamine
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Vaz, W.L.C., Hallmann, D., Clegg, R.M. et al. A comparison of the translational diffusion of a normal and a membrane-spanning lipid in Lα phase 1-palmitoyl-2-oleoylphosphatidylcholine bilayers. Eur Biophys J 12, 19–24 (1985). https://doi.org/10.1007/BF00254091
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DOI: https://doi.org/10.1007/BF00254091