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Prothymosin α gene in humans: organization of its promoter region and localization to chromosome 2

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Abstract

A genomic clone encoding prothymosin α (gene symbol: PTMA), a nuclear-targeted protein associated with cell proliferation, was isolated and the 5′-regulatory region subcloned and sequenced. Because of previously reported discrepancies between several cDNA clones and a genomic clone for prothymosin α, we determined the sequence of the first exon and of a 1.7-kb region 5′ to the first exon. The sequence of the genomic clone reported here corresponds to the published cDNA sequences, suggesting that the previously noted discrepancies may be due to genetic polymorphism in this region. In addition, our sequence data extend the known 5′-upstream sequence by an additional 1.5 kb allowing the identification of numerous, potential cis-acting regulatory sites. This 5′-flanking cloned probe permitted us to localize the protothymosin gene to chromosome 2 in humans.

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Author notes

  1. C. Panneerselvam

    Present address: Department of Biochemistry, Madras University, Taramani Campus, Madras, India

  2. M. Clinton

    Present address: Department of Reproduction and Developmental Biology, Institute of Animal Physiology and Genetic Research, Edinburgh, UK

  3. M. Frangou-Lazaridis

    Present address: Laboratory of Biological Chemistry, University of Ioannina Medical School, Ioannina, Greece

Authors and Affiliations

  1. Division of Geriatrics and Gerontology, Department of Medicine, Cornell University Medical College, 10021, New York, NY, USA

    P. Szabo, D. Weksler & E. Whittington

  2. Department of Biochemistry, Cornell University Medical College, 10021, New York, NY, USA

    C. Panneerselvam, M. Clinton, M. Frangou-Lazaridis & B. L. Horecker

  3. Immunogenetics Laboratory, Memorial Sloan-Kettering Cancer Center, 10021, New York, NY, USA

    A. Selvakumar

  4. Department of Genetics, Long Island College Hospital, 11201, Brooklyn, NY, USA

    M. J. Macera

  5. Institut für Humangenetik, Klinikum der Philipps-Universität Marburg, Marburg, Germany

    K.-H. Grzeschik

Authors
  1. P. Szabo
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  2. C. Panneerselvam
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  3. M. Clinton
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  4. M. Frangou-Lazaridis
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  5. D. Weksler
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  6. E. Whittington
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  7. M. J. Macera
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  8. K.-H. Grzeschik
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  9. A. Selvakumar
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  10. B. L. Horecker
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Szabo, P., Panneerselvam, C., Clinton, M. et al. Prothymosin α gene in humans: organization of its promoter region and localization to chromosome 2. Hum Genet 90, 629–634 (1993). https://doi.org/10.1007/BF00202480

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  • DOI: https://doi.org/10.1007/BF00202480

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