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Isolation and partial characterization of nigrin b, a non-toxic novel type 2 ribosome-inactivating protein from the bark ofSambucus nigra L.

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Abstract

The bark ofSambucus nigra L. contains a non-toxic novel type 2 ribosome-inactivating protein that we named nigrin b.In vitro, nigrin b strongly inhibited mammalian protein synthesis but did not affect plant nor bacterial protein synthesis. The protein (M r 58 000) contains two subunits, A (M r 26 000) and B (M r 32 000); linked by disulphide bridge(s). Nigrin b was found to be an rRNA N-glycosidase of the rRNA of intact mammalian ribosomes and shares a very good N-terminal amino-acid sequence homology with the anti-HIV-1 proteins TAP 29 and trichosanthin.

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Authors and Affiliations

  1. Departamento de Bioquimíca y Biología Molecular, Facultad de Ciencias, Universidad de Valladolid, E-47005, Valladolid, Spain

    Tomás Girbés, Lucía Citores, J. Miguel Ferreras, M. Angeles Rojo, Rosario Iglesias, Raquel Muñoz & F. Javier Arias

  2. Insituto de Oftalmobiología Aplicada, Universidad de Valladolid, E-47005, Valladolid, Spain

    Margarita Calonge

  3. Servicio de Endocrinología, Centro Ramón y Cajal, Carretera de Colmenar Viejo km 9.1, E-28034, Madrid, Spain

    J. Ramón García & Enrique Méndez

Authors
  1. Tomás Girbés
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  2. Lucía Citores
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  3. J. Miguel Ferreras
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  4. M. Angeles Rojo
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  5. Rosario Iglesias
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  6. Raquel Muñoz
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  7. F. Javier Arias
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  8. Margarita Calonge
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  9. J. Ramón García
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  10. Enrique Méndez
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Girbés, T., Citores, L., Miguel Ferreras, J. et al. Isolation and partial characterization of nigrin b, a non-toxic novel type 2 ribosome-inactivating protein from the bark ofSambucus nigra L.. Plant Mol Biol 22, 1181–1186 (1993). https://doi.org/10.1007/BF00028990

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  • DOI: https://doi.org/10.1007/BF00028990

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