Skip to main content
SpringerLink
Log in

Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer

  • Regular Paper
  • Published:
Photosynthesis Research Aims and scope Submit manuscript

Abstract

A newly developed fluorescence measuring system is employed for the recording of chlorophyll fluorescence induction kinetics (Kautsky-effect) and for the continuous determination of the photochemical and non-photochemical components of fluorescence quenching. The measuring system, which is based on a pulse modulation principle, selectively monitors the fluorescence yield of a weak measuring beam and is not affected even by extremely high intensities of actinic light. By repetitive application of short light pulses of saturating intensity, the fluorescence yield at complete suppression of photochemical quenching is repetitively recorded, allowing the determination of continuous plots of photochemical quenching and non-photochemical quenching. Such plots are compared with the time courses of variable fluorescence at different intensities of actinic illumination. The differences between the observed kinetics are discussed. It is shown that the modulation fluorometer, in combination with the application of saturating light pulses, provides essential information beyond that obtained with conventional chlorophyll fluorometers.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Krause GH and Weis E (1984) Chlorophyll fluorescence as a tool in plant physiology. II. Interpretation of fluorescence signals. Photosynthe Res 5: 139–157

    Google Scholar 

  2. Briantais JM, Vernotte C, Krause GH and Weis E (1985) Chlorophyll fluorescence of higher plants: Chloroplasts and leaves. In: Govindjee Amesz J. and Fork DC (eds) Light Emission by Plants and Bacteria. New York: Academic Press.

    Google Scholar 

  3. Renger G and Schreiber U (1985) Practical applications of fluorometric methods to algae and higher plant research. In: Govindjee Amesz J. and Fork DC (eds) Light Emission by Plants and Bacteria. New York: Academic Press.

    Google Scholar 

  4. Briantais JM, Vernotte C, Picaud M and Krause GH (1980) Chlorophyll fluorescence as a probe for the determination of the photoinduced proton gradient in isolated chloroplasts. Biochim Biophys Acta 591: 198–202

    Google Scholar 

  5. Krause GH, Briantais JM and Vernotte C (1982) Photoinduced quenching of chlorophyll fluorescence in intact chloroplasts and algae. Resolution into two components. Biochim Biophys Acta 679: 116–124

    Google Scholar 

  6. Bradbury M and Baker NR (1981) Analysis of the slow phases of the in vivo chlorophyll fluorescence induction curve. Changes in the redox state of photosystem II electron acceptors and fluorescence emission from photosystems I and II. Biochim Biophys Acta 63: 542–551

    Google Scholar 

  7. Quick WP and Horton P (1984) Studies on the induction of chlorophyll fluorescence quenching by redox state and transthylakoid pH gradient. Proc R Soc Lond B 220: 371–382

    Google Scholar 

  8. Horton P (1983) Relations between electron transport and carbon assimilation: simultaneous measurement of chlorophyll fluorescence, transthylakoid pH gradient and O2 evolution in isolated chloroplasts. Proc R Soc Lond B 217: 405–416

    Google Scholar 

  9. Dietz K-J, Schreiber U and Heber U (1985) The relationship between the redox state of QA and photosynthesis in leaves at various carbon dioxide, oxygen and light regimes. Planta 166: 219–226

    Google Scholar 

  10. Schreiber U (1983) Technical review. Chlorophyll fluorescence yield changes as a tool in plant physiology. I. The measuring system. Photosynth Res 4: 361–373

    Google Scholar 

  11. Bradbury M and Baker NR (1984) A quantitative analysis of the chlorophyll fluorescence induction curve from pea leaves. In: Sybesma C (ed) Advances in Photosynthesis Research, Vol. III, 4, pp 327–330. The Hague: Martinus Nijhoff/Dr W. Junk Publishers

    Google Scholar 

  12. Carillo N, Lucero HA and Vallejos RH (1981) Light modulation of chloroplast membrane bound ferredoxin-NADP+ oxidoreductase. J Bio Chem 256: 1058–1059

    Google Scholar 

  13. Horton P (1983) Effects of changes in the capacity for photosynthetic electron transfer and photophosphorylation on the kinetics of fluorescence induction in isolated chloroplasts. Biochim Biophys Acta 724: 404–410

    Google Scholar 

  14. Briantais JM, Vernotte C, Picaud M and Krause GH (1979) A quantitative study of the slow decline of chlorophyll fluorescence in isolated chloroplasts. Biochim Biophys Acta: 128–138

Download references

Author information

Authors and Affiliations

  1. Institut für Botanik, Mittlerer Dallenbergweg 64, D-8700, Würzburg, FRG

    U. Schreiber, U. Schliwa & W. Bilger

Authors
  1. U. Schreiber
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. U. Schliwa
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. W. Bilger
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Schreiber, U., Schliwa, U. & Bilger, W. Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer. Photosynth Res 10, 51–62 (1986). https://doi.org/10.1007/BF00024185

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00024185

Key words

Search

Navigation