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Evaluating the Metabolic Impact of Hypoxia on Pancreatic Cancer Cells

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Book cover Hypoxia

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1742))

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Abstract

Hypoxia is frequently observed in human cancers and induces global metabolic reprogramming that includes an increase in glucose uptake and glycolysis, alterations in NAD(P)H/NAD(P)+ and intracellular ATP levels, and increased utilization of glutamine as the major precursor for fatty acid synthesis. In this chapter, we describe in detail various physiological assays that have been adopted to study the metabolic shift propagated by exposure to hypoxic conditions in pancreatic cell culture model that includes glucose uptake, glutamine uptake, and lactate release by pancreatic cancer cell lines. We have also elaborated the assays to evaluate the ratio of NAD(P)H/NAD(P)+ and intracellular ATP estimation using the commercially available kit to assess the metabolic state of cancer cells.

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Authors and Affiliations

  1. The Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, NE, USA

    Divya Murthy, Enza Vernucci, Gennifer Goode, Jaime Abrego & Pankaj K. Singh

  2. Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE, USA

    Pankaj K. Singh

  3. Department of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, NE, USA

    Pankaj K. Singh

  4. Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE, USA

    Pankaj K. Singh

Authors
  1. Divya Murthy
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  2. Enza Vernucci
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  3. Gennifer Goode
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  4. Jaime Abrego
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  5. Pankaj K. Singh
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Corresponding author

Correspondence to Pankaj K. Singh .

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Editors and Affiliations

  1. Department of Neurosurgery, Clinical Neurosciences Center, University of Utah, Salt Lake City, Utah, USA

    L. Eric Huang

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Murthy, D., Vernucci, E., Goode, G., Abrego, J., Singh, P.K. (2018). Evaluating the Metabolic Impact of Hypoxia on Pancreatic Cancer Cells. In: Huang, L. (eds) Hypoxia. Methods in Molecular Biology, vol 1742. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7665-2_8

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  • DOI: https://doi.org/10.1007/978-1-4939-7665-2_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7664-5

  • Online ISBN: 978-1-4939-7665-2

  • eBook Packages: Springer Protocols

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