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West Nile Virus pp 151–163Cite as

Techniques for Experimental Infection of Mosquitoes with West Nile Virus

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1435))

Abstract

West Nile virus is a typical mosquito-borne flavivirus, and it is transmitted between mosquitoes and birds in nature. As an incidental host, humans are susceptible to WNV infection. WNV infection in humans can result in fever, meningitis, and encephalitis. Approved human vaccines or therapies are not available for WNV infection. In this chapter, we focus on the techniques for WNV infection and detection in mosquitoes. The technical details include: (1) WNV infection in cell culture; (2) Mosquito rearing; (3) WNV infection in mosquitoes via thoracic microinjection; (4) Detection of WNV infection in mosquitoes; (5) Determination of WNV M.I.D50 in mosquitoes; (6) WNV infection in mosquitoes via membrane blood feeding; (7) WNV infection via blood feeding on mice; (8) Immunofluorescence staining of WNV infected mosquito tissues.

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Acknowledgement

This work was funded by grants from National Natural Science Foundation of China (81301412 and 81422028), the National Key Technologies Research and Development Program of China (2013ZX10004-610), National Key Basic Research Program of MOST (2013CB911500). G.C. is a Newton Advanced Fellow awarded by the Academy of Medical Sciences and the Newton Fund.

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Authors and Affiliations

  1. Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing, 100084, People’s Republic of China

    Yang Liu & Gong Cheng Ph.D.

  2. Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, 310003, People’s Republic of China

    Yang Liu & Gong Cheng Ph.D.

  3. School of Life Science, Tsinghua University, Beijing, 100084, People’s Republic of China

    Yang Liu

Authors
  1. Yang Liu
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  2. Gong Cheng Ph.D.
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Corresponding author

Correspondence to Gong Cheng Ph.D. .

Editor information

Editors and Affiliations

  1. Dept. of Path., MicroBio., and Immun., Univ. of South Carolina School of Med., Columbia, South Carolina, USA

    Tonya M. Colpitts

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Liu, Y., Cheng, G. (2016). Techniques for Experimental Infection of Mosquitoes with West Nile Virus. In: Colpitts, T. (eds) West Nile Virus. Methods in Molecular Biology, vol 1435. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3670-0_13

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  • DOI: https://doi.org/10.1007/978-1-4939-3670-0_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3668-7

  • Online ISBN: 978-1-4939-3670-0

  • eBook Packages: Springer Protocols

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